Expression of soybean Kunitz trypsin inhibitor gene SKTI in Dunaliella salina

Journal of Applied Phycology

Volumn 25, Issue 1, 2013, Pages 139-144

  Chai, Xiao Jie ^a   Chen, Hui Xia ^a   Xu, Wen Qi ^a   Xu, Ya Wei ^b  

^a DALIAN OCEAN UNIVERSITY   (China)

^b Jilin College of Agricultural Science and Technology   (China)

Author keywords

Dunaliella salina; LiAc PEG mediated method; Soybean Kunitz trypsin inhibitor gene SKTI

Indexed keywords

CHLOROPHYTA; DUNALIELLA SALINA; GLYCINE MAX;

EID: 84872499682     PISSN: 09218971     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10811-012-9847-8     Document Type: Article

References (30)

1. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248-254.
2. Cabanes-Macheteau M, Fitchette-Laine AC, Loutelie-Bourhis C, Lange C, Vine N, Ma J, Lerouge P, Faye L (1999) N-Glycosylation of a mouse IgG expressed in transgenic tobacco plants. Glycobiology 9: 365-372.
3. Chen MY (1995) Biological food culture. China Agriculture, Beijing, pp 62-63, (In Chinese).
4. Cramer CL, Boothe JG, Oishi KK (1999) Transgenic plants for therapeutic proteins: linking upstream and downstream strategies. Curr Top Microbiol Immunol 240: 95-118.
5. El-Sheekh MM (1999) Stable transformation of the intact cells of Chlorella kessleri with high velocity microprojectiles. Biol Plantarum 42: 209-216.
6. Falco MC, Silva-Filho MC (2003) Expression of soybean proteinase inhibitors in transgenic sugarcane plants: effects on natural defense against Diatraea saccharalis. Plant Physiol Biochem 41: 761-766.
7. Feng SY, Jia YL, Liu HT et al (2007) Transformation of Dunaliella salina by using glass beads-a novel transformation method. Chin J Biotechnol 23(2): 358-362 (In Chinese).
8. Fitzpatrick KL, Tyerman SD, Kaiser BN (2008) Molybdate transport through the plant sulfate transporter SHST1. FEBS Lett 582: 1508-1513.
9. Franco OL, Dias SC, Magalhães CP, Monteiro ACS, Jr Bloch C, Melo FR, Oliveira-Neto OB, Monnerat RG, Grossi-de-Sá MF (2004) Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (Anthonomus grandis). Phytochemistry 65: 81-89.
10. Franklin SE, Mayfield SP (2004) Prospects for molecular farming in the green alga Chlamydomonas. Curr Opin Plant Biol 7: 159-165.
11. Geng DG, Wang YQ, Li WB et al (2002) Transient expression of GUS gene in Dunaliella salina. High Technol Lett 12: 35-39 (In Chinese).
12. Geng D, Wang Y, Wang P, Li W, Sun Y (2003) Stable expression of hepatitis B surface antigen in Dunaliella salina (Chlorophyta). J Appl Phycol 15: 451-456.
13. Gietz RD, Woods RA (2001) Genetic transformation of yeast. Biotechniques 30: 816-831.
14. Harlow E, Lane D (1999) Using antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press.
15. He Q (2003) Microalgae as platforms for recombinant proteins. In: Richmond A (ed) Handbook of microalgal culture: biotechnology and applied phycology. Blackwell, Oxford, pp 471-484.
16. Hsieh CC, Hernández-Ledema B, Jeong HJ, Park JH, de Lumen BO (2010) Complementary roles in cancer prevention: protease inhibitor makes the cancer preventive peptide lunasin bioavailable. PLoS One 5(1): e8890.
17. Kindle KL (1990) High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 87: 1228-1232.
18. Kobayashi H, Suzuki M, Kanayama N, Terao T (2004) A soybean Kunitz trypsin inhibitor suppresses ovarian cancer cell invasion by blocking urokinase upregulation. Clin Exp Metastasis 21: 159-166.
19. Koide T, Tsunasawa S, Ikenaka T (1993) Studies on soybean trypsin inhibitors and amino acid sequence around the reactive site of soybean trypsin inhibitor (Kunitz). Eur J Biochem 32: 408-416.
20. Li J, Yan HX, Qu DJ (2007) Construction of an EGFP eukaryotic expression vector by SOEing and its transformation to Dunaliella Salina. Acta Hydrobiol Sin 31(4): 546-551 (In Chinese).
21. Li J, Brader G, Palva ET (2008) Kunitz trypsin inhibitor: an antagonist of cell death triggered by phytopathogens and fumonisin B1 in arabidopsis. Mol Plant 1: 482-495.
22. Lippman SM, Matrisian LM (2000) Protease inhibitors in oral carcinogenesis and chemoprevention. Clin Cancer Res 6: 4599-4603.
23. Lv YM, Xie H, Niu XL et al (2002) Transfer of bar gene to Dunaliella salina by microprojectile delivery and detection of transgenic alga. J Zhengzhou Univ 12(2): 35-39 (In Chinese).
24. Paju A, Hotakainen K, Cao Y, Laurila T, Gadaleanu V, Hemminki A, Stenman U, Bjartell A (2007) Increased expression of tumor-associated trypsin inhibitor, TATI, in prostate cancer and in androgen-independent 22Rv1 cells. Eur Urol 52: 1670-1681.
25. Rashed NA, Macdonald MH, Matthews BF (2008) Protease inhibitor expression in soybean roots exhibiting susceptible and resistant interactions with soybean cyst nematode. J Nematol 40: 138-146.
26. Ribeiro JK, Cunha DD, Fook JM, Sales MP (2010) New properties of the soybean trypsin inhibitor: inhibition of human neutrophil elastase and its effect on acute pulmonary injury. Eur J Pharmacol 644: 238-244.
27. Sala F, Manuela Rigano M, Barbante A, Basso B, Walmsley AM, Castiglione S (2003) Vaccine antigen production in transgenic plants: strategies, gene constructs and perspectives. Vaccine 21: 803-808.
28. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press.
29. Song SI, Kim CH, Baek SJ, Choi YD (1993) Nucleotide sequences of cDNAs encoding the precursors for soybean (Glycine max). Plant Physiol 101: 1401-1402.
30. Walker TL, Purton S, Becker DK, Collet C (2005) Microalgae as bioreactors. Plant Cell Rep 24: 629-641.