Transformation of Dunaliella salina by using glass beads - A novel transformation method

Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

Volumn 23, Issue 2, 2007, Pages 358-362

  Feng, Shu Ying ^a   Jia, Yan Long ^a   Liu, Hong Tao ^a   Li, Jie ^a   Xue, Le Xun ^a  

^a THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIVERSITY   (China)

Author keywords

Dunaliella salina; Glass beads; Novel transformation method

Indexed keywords

DUNALIELLA SALINA;

BETA GLUCURONIDASE; DNA; GLASS; MACROGOL DERIVATIVE; MICROSPHERE;

ARTICLE; CHEMISTRY; CYTOCHEMISTRY; GENETIC ENGINEERING; GENETIC TRANSFORMATION; GENETICS; GREEN ALGA; METABOLISM; METHODOLOGY; PLASMID; TIME;

ALGAE, GREEN; DNA; GENETIC ENGINEERING; GLASS; GLUCURONIDASE; HISTOCYTOCHEMISTRY; MICROSPHERES; PLASMIDS; POLYETHYLENE GLYCOLS; TIME FACTORS; TRANSFORMATION, GENETIC;

EID: 66849128633     PISSN: 10003061     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Ben-Amotz A, Shaish A, Avron M. The biotechnology of cultivating Dunaliella for production of β-Carotene rich algae. Bioresource Technol, 1991, 38: 233-235.
2. Walker TL, Purton S, Becker DK, et al. Mocroalgae as bioreactors. Plant Cell Rep, 2005, 24(11): 629-641.
3. Nikookar K, Moradshahi A, Hosseini L. Physiological responses of Dunaliella salina and Dunaliella tertiolecta to copper toxicity. Biomol Eng, 2005, 22(4): 141-146.
4. Xue LX, Pan WD, Jiang GZ, et al. Dunaliella salina as a bioreactor, China patent, CN00131217.0.
5. Tan C, Qin S, Zhang Q, et al. Establishment of a micro-particle bombardment transformation system for Dunaliella salina. Microbiol, 2005, 43(4): 361-365.
6. Sun Y, Yang Z, Gao X, et al. Expression of foreign genes in dunaliella by electroporation. Mol Biotechnol, 2005, 30(3): 185-192.
7. Kindle KL, Schnell RA, Fernandez E, et al. Stable nuclear transformation of chlamydomonas using the chlamydomonas gene for nitrate reductase. The Journal of Cell Biology, 1989, 109: 2589-2601.
8. Brown LE, Sprecher SL, Keller LR. Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation. Mol Cell Biol, 1991, 11: 2328-2332.
9. Kumar SV, Misquitta RW, Reddy VS, et al. Genetic transformation of the green alga - Chlamydomonas reinhardtii by Agrobacterium tumefaciens. Plant Science, 2004, 166: 731-738.
10. Dunahay TG. Transformation of Chlamydomonas reinhardtii with silicon carbide whiskers. Biotechniques, 1993, 15: 452-460.
11. Kindle KL. High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA, 1990, 87: 1228-1232.
12. Fisher M, Pick U, Zamir A. A salt-induced 60-kilodalton plasma membrane protein plays a potential role in the extreme halotolerance of the alga Dunaliella. Plant Physiol, 1994, 106: 1359-1365
13. Jefferson RA, Knvanagh TA, Bevan MW. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J, 1987, 6: 3901-3907.
14. Geng DG, Wang YQ, Li WB, et al. Transient expression of GUS gene in Dunaliella salina. High Technology Letters, 2002, 12(2): 35-39.