Recombinant expression and purification of a tumor-targeted toxin in Bacillus anthracis

Biochemical and Biophysical Research Communications

Volumn 430, Issue 1, 2013, Pages 150-155

  Bachran, Christopher ^a   Abdelazim, Suzanne ^a   Fattah, Rasem J ^a   Liu, Shihui ^a   Leppla, Stephen H ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Diphtheria toxin; Endotoxin; Immunotoxin; Lipopolysaccharide; Tobacco etch virus protease; Tumor therapy

Indexed keywords

DIPHTHERIA TOXIN; EPIDERMAL GROWTH FACTOR RECEPTOR; LIPOPOLYSACCHARIDE; TRANSFORMING GROWTH FACTOR ALPHA;

AMINO TERMINAL SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BACILLUS ANTHRACIS; BACTERIAL STRAIN; CANCER CELL; CYTOTOXICITY; ESCHERICHIA COLI; HEAD AND NECK CANCER; LETHAL GENE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SECRETION;

ANTIGENS, BACTERIAL; BACILLUS ANTHRACIS; BACTERIAL TOXINS; CELL LINE, TUMOR; CHROMATOGRAPHY, ION EXCHANGE; DIPHTHERIA TOXIN; HUMANS; IMMUNOTOXINS; RECOMBINANT FUSION PROTEINS; TRANSFORMING GROWTH FACTOR ALPHA;

BACILLUS ANTHRACIS; ESCHERICHIA COLI; POSIBACTERIA; TOBACCO ETCH VIRUS;

EID: 84872401196     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.11.055     Document Type: Article

References (21)

1. Pomerantsev A.P., Pomerantseva O.M., Moayeri M., Fattah R., Tallant C., Leppla S.H. A Bacillus anthracis strain deleted for six proteases serves as an effective host for production of recombinant proteins. Protein Expr. Purif. 2011, 80:80-90.
2. Arora N., Leppla S.H. Fusions of anthrax toxin lethal factor with shiga toxin and diphtheria toxin enzymatic domains are toxic to mammalian cells. Infect. Immun. 1994, 62:4955-4961.
3. Hobson J.P., Liu S., Rono B., Leppla S.H., Bugge T.H. Imaging specific cell-surface proteolytic activity in single living cells. Nat. Methods 2006, 3:259-261.
4. Abi-Habib R.J., Singh R., Liu S., Bugge T.H., Leppla S.H., Frankel A.E. A urokinase-activated recombinant anthrax toxin is selectively cytotoxic to many human tumor cell types. Mol. Cancer Ther. 2006, 5:2556-2562.
5. Liu S., Redeye V., Kuremsky J.G., Kuhnen M., Molinolo A., Bugge T.H., Leppla S.H. Intermolecular complementation achieves high-specificity tumor targeting by anthrax toxin. Nat. Biotechnol. 2005, 23:725-730.
6. Fuchs H., Bachran C. Targeted tumor therapies at a glance. Curr. Drug Targets 2009, 10:89-93.
7. Hetzel C., Bachran C., Tur M.K., Fuchs H., Stocker M. Improved immunotoxins with novel functional elements. Curr. Pharm. Des. 2009, 15:2700-2711.
8. Rosovitz M.J., Schuck P., Varughese M., Chopra A.P., Mehra V., Singh Y., McGinnis L.M., Leppla S.H. Alanine scanning mutations in domain 4 of anthrax toxin protective antigen reveal residues important for binding to the cellular receptor and to a neutralizing monoclonal antibody. J. Biol. Chem. 2003, 278:30936-30944.
9. Tropea J.E., Cherry S., Waugh D.S. Expression and purification of soluble His(6)-tagged TEV protease. Methods Mol. Biol. 2009, 498:297-307.
10. Yeudall W.A., Crawford R.Y., Ensley J.F., Robbins K.C. MTS1/CDK4I is altered in cell lines derived from primary and metastatic oral squamous cell carcinoma. Carcinogenesis 1994, 15:2683-2686.
11. Park S., Leppla S.H. Optimized production and purification of Bacillus anthracis lethal factor. Protein Expr. Purif. 2000, 18:293-302.
12. Gao Z., Li Z., Zhang Y., Huang H., Li M., Zhou L., Tang Y., Yao B., Zhang W. High-level expression of the Penicillium notatum glucose oxidase gene in Pichia pastoris using codon optimization. Biotechnol. Lett. 2012, 34:507-514.
13. Wu X.C., Lee W., Tran L., Wong S.L. Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases. J. Bacteriol. 1991, 173:4952-4958.
14. Jiang X., Oohira K., Iwasaki Y., Nakano H., Ichihara S., Yamane T. Reduction of protein degradation by use of protease-deficient mutants in cell-free protein synthesis system of Escherichia coli. J. Biosci. Bioeng. 2002, 93:151-156.
15. Biedendieck R., Borgmeier C., Bunk B., Stammen S., Scherling C., Meinhardt F., Wittmann C., Jahn D. Systems biology of recombinant protein production using Bacillus megaterium. Methods Enzymol. 2011, 500:165-195.
16. Sun C., Liang J., Shi R., Gao X., Zhang R., Hong F., Yuan Q., Wang S. Tobacco etch virus protease retains its activity in various buffers and in the presence of diverse additives. Protein Expr. Purif. 2012, 82:226-231.
17. Kapust R.B., Tozser J., Copeland T.D., Waugh D.S. The P1' specificity of tobacco etch virus protease. Biochem. Biophys. Res. Commun. 2002, 294:949-955.
18. Ilk N., Schumi C.T., Bohle B., Egelseer E.M., Sleytr U.B. Expression of an endotoxin-free S-layer/allergen fusion protein in gram-positive Bacillus subtilis 1012 for the potential application as vaccines for immunotherapy of atopic allergy. Microb. Cell Fact. 2011, 10:6.
19. Lichtman S.N., Wang J., Lemasters J.J. LPS receptor CD14 participates in release of TNF-alpha in RAW 264.7 and peritoneal cells but not in kupffer cells. Am. J. Physiol. 1998, 275:G39-46.
20. Yoo G.H., Subramanian G., Piechocki M.P., Ensley J.F., Kucuk O., Tulunay O.E., Lonardo F., Kim H., Won J., Stevens T., Lin H.S. Effect of docetaxel on the surgical tumor microenvironment of head and neck cancer in murine models. Arch. Otolaryngol. Head Neck Surg. 2008, 134:735-742.
21. Bachran C., Schneider S., Riese S.B., Bachran D., Urban R., Schellmann N., Zahn C., Sutherland M., Fuchs H. A lysine-free mutant of epidermal growth factor as targeting moiety of a targeted toxin. Life Sci. 2011, 88:226-232.