메뉴 건너뛰기




Volumn 97, Issue 1, 2013, Pages 153-158

Comparative characterization of recombinant ZZ protein-alkaline phosphatase and its application in enzyme immunoassays

Author keywords

Alkaline phosphatase; Chemical conjugate; Enzyme immunoassay; Fusion protein; IgG; ZZ protein

Indexed keywords

ALKALINE PHOSPHATASE; BACTERIAL SYSTEMS; ENZYMATIC ACTIVITIES; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; EQUILIBRIUM DISSOCIATION CONSTANT; FUSION COMPLEX; FUSION PROTEINS; IGG; MICROBIAL SYSTEM; QUANTITATIVE COMPARISON; REPORTER ENZYMES;

EID: 84872353302     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4303-x     Document Type: Article
Times cited : (7)

References (25)
  • 1
    • 33745726784 scopus 로고    scopus 로고
    • Extracellular secretion of Escherichia coli alkaline phosphatase with a C-terminal tag by type i secretion system: Purification and biochemical characterization
    • 10.1093/protein/gzl017 10.1093/protein/gzl017 1:CAS:528: DC%2BD28Xmt1Wktbg%3D
    • Angkawidjaja C, Kuwahara K, Omori K, Koga Y, Takano K, Kanaya S (2006) Extracellular secretion of Escherichia coli alkaline phosphatase with a C-terminal tag by type I secretion system: purification and biochemical characterization. Protein Eng Des Sel 19:337-343. doi: 10.1093/protein/gzl017
    • (2006) Protein Eng des Sel , vol.19 , pp. 337-343
    • Angkawidjaja, C.1    Kuwahara, K.2    Omori, K.3    Koga, Y.4    Takano, K.5    Kanaya, S.6
  • 2
    • 72449163625 scopus 로고    scopus 로고
    • Expanding the chemical biologist's tool kit: Chemical labelling strategies and its applications
    • 10.2174/092986709789760706 10.2174/092986709789760706 1:CAS:528:DC%2BC3cXnt1Wk
    • Chattopadhaya S, Abu Bakar FB, Yao SQ (2009) Expanding the chemical biologist's tool kit: chemical labelling strategies and its applications. Curr Med Chem 16:4527-4543. doi: 10.2174/092986709789760706
    • (2009) Curr Med Chem , vol.16 , pp. 4527-4543
    • Chattopadhaya, S.1    Abu Bakar, F.B.2    Yao, S.Q.3
  • 3
    • 0028371166 scopus 로고
    • An expression system for secretion and purification of a genetically engineered thermostable chimera of protein A and alkaline phosphatase
    • 10.1006/prep.1994.1013 1:CAS:528:DyaK2cXhvVChsrk%3D
    • Chowdhury PS, Kushwaha A, Abrol S, Chaudhary VK (1994) An expression system for secretion and purification of a genetically engineered thermostable chimera of protein A and alkaline phosphatase. Protein Expr Purif 5:89-95
    • (1994) Protein Expr Purif , vol.5 , pp. 89-95
    • Chowdhury, P.S.1    Kushwaha, A.2    Abrol, S.3    Chaudhary, V.K.4
  • 4
    • 0035866630 scopus 로고    scopus 로고
    • A genetically engineered fusion protein with horseradish peroxidase as a marker enzyme for use in competitive immunoassays
    • 10.1021/ac000684t 10.1021/ac000684t 1:CAS:528:DC%2BD3MXhtVymsrY%3D
    • Grigorenko V, Andreeva I, Börchers T, Spener F, Egorov A (2001) A genetically engineered fusion protein with horseradish peroxidase as a marker enzyme for use in competitive immunoassays. Anal Chem 73:1134-1139. doi: 10.1021/ac000684t
    • (2001) Anal Chem , vol.73 , pp. 1134-1139
    • Grigorenko, V.1    Andreeva, I.2    Börchers, T.3    Spener, F.4    Egorov, A.5
  • 5
    • 84862909230 scopus 로고    scopus 로고
    • Molecular mechanism of the effects of salt and pH on the affinity between protein A and human immunoglobulin G1 revealed by molecular simulations
    • 10.1021/jp205770p 10.1021/jp205770p 1:CAS:528:DC%2BC3MXhsFKhu73J
    • Huang B, Liu FF, Dong XY, Sun Y (2012) Molecular mechanism of the effects of salt and pH on the affinity between protein A and human immunoglobulin G1 revealed by molecular simulations. J Phys Chem B 116:424-433. doi: 10.1021/jp205770p
    • (2012) J Phys Chem B , vol.116 , pp. 424-433
    • Huang, B.1    Liu, F.F.2    Dong, X.Y.3    Sun, Y.4
  • 7
    • 0031884383 scopus 로고    scopus 로고
    • All individual domains of staphylococcal protein A show Fab binding
    • 10.1016/S0928-824 10.1016/S0928-8244(97)00108-9 1:CAS:528: DyaK1cXht1Okur0%3D
    • Jansson B, Uhlen M, Nygren PA (1998) All individual domains of staphylococcal protein A show Fab binding. FEMS Immunol Med Microbiol 20:69-78. doi: 10.1016/S0928-824
    • (1998) FEMS Immunol Med Microbiol , vol.20 , pp. 69-78
    • Jansson, B.1    Uhlen, M.2    Nygren, P.A.3
  • 8
    • 0030043942 scopus 로고    scopus 로고
    • The mechanism of binding staphylococcal protein A to immunoglobin G does not involve helix unwinding
    • 10.1021/bi9512814 10.1021/bi9512814 1:CAS:528:DyaK2MXpvFWhsrk%3D
    • Jendeberg L, Tashiro M, Tejero R, Lyons BA, Uhlén M, Montelione GT, Nilsson B (1996) The mechanism of binding staphylococcal protein A to immunoglobin G does not involve helix unwinding. Biochemistry 35:22-31. doi: 10.1021/bi9512814
    • (1996) Biochemistry , vol.35 , pp. 22-31
    • Jendeberg, L.1    Tashiro, M.2    Tejero, R.3    Lyons, B.A.4    Uhlén, M.5    Montelione, G.T.6    Nilsson, B.7
  • 9
    • 0029929019 scopus 로고    scopus 로고
    • PDAP2: A vector for construction of alkaline phosphatase fusion-proteins
    • 10.1016/1380-2933(96)00040-1 1:CAS:528:DyaK28XkslGrs7c%3D
    • Kerschbaumer RJ, Hirschl S, Schwager C, Ibl M, Himmler G (1996) pDAP2: a vector for construction of alkaline phosphatase fusion-proteins. Immunotechnology 2:145-150
    • (1996) Immunotechnology , vol.2 , pp. 145-150
    • Kerschbaumer, R.J.1    Hirschl, S.2    Schwager, C.3    Ibl, M.4    Himmler, G.5
  • 10
    • 0031194670 scopus 로고    scopus 로고
    • Single-chain Fv fusion proteins suitable as coating and detecting reagents in a double antibody sandwich enzyme-linked immunosorbent assay
    • 10.1006/abio.1997.2171 10.1006/abio.1997.2171 1:CAS:528: DyaK2sXktF2rsb0%3D
    • Kerschbaumer RJ, Hirschl S, Kaufmann A, Ibl M, Koenig R, Himmler G (1997) Single-chain Fv fusion proteins suitable as coating and detecting reagents in a double antibody sandwich enzyme-linked immunosorbent assay. Anal Biochem 249:219-227. doi: 10.1006/abio.1997.2171
    • (1997) Anal Biochem , vol.249 , pp. 219-227
    • Kerschbaumer, R.J.1    Hirschl, S.2    Kaufmann, A.3    Ibl, M.4    Koenig, R.5    Himmler, G.6
  • 11
    • 28044462393 scopus 로고    scopus 로고
    • Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA)
    • 10.1373/clinchem.2005.051532 10.1373/clinchem.2005.051532 1:CAS:528:DC%2BD2MXht1yksL3N
    • Lequin RM (2005) Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA). Clin Chem 51:2415-2418. doi: 10.1373/clinchem.2005.051532
    • (2005) Clin Chem , vol.51 , pp. 2415-2418
    • Lequin, R.M.1
  • 12
    • 77954818994 scopus 로고    scopus 로고
    • Production and characterization of a single-chain Fv antibody-alkaline phosphatase fusion protein specific for clenbuterol
    • 10.1007/s12033-010-9240-2 10.1007/s12033-010-9240-2 1:CAS:528: DC%2BC3cXktVWqtLY%3D
    • Liu X, Wang H, Liang Y, Yang J, Zhang H, Lei H, Shen Y, Sun Y (2010) Production and characterization of a single-chain Fv antibody-alkaline phosphatase fusion protein specific for clenbuterol. Mol Biotechnol 45:56-64. doi: 10.1007/s12033-010-9240-2
    • (2010) Mol Biotechnol , vol.45 , pp. 56-64
    • Liu, X.1    Wang, H.2    Liang, Y.3    Yang, J.4    Zhang, H.5    Lei, H.6    Shen, Y.7    Sun, Y.8
  • 14
    • 0026694313 scopus 로고
    • Enzyme-immunoassay techniques: An overview
    • 10.1016/0022-1759(92)90061-W 1:CAS:528:DyaK38XltVOksrk%3D
    • Porstmann T, Kiessig ST (1992) Enzyme-immunoassay techniques: an overview. J Immunol Methods 150:5-21
    • (1992) J Immunol Methods , vol.150 , pp. 5-21
    • Porstmann, T.1    Kiessig, S.T.2
  • 15
    • 0036000069 scopus 로고    scopus 로고
    • Single-chain Fv antibody-alkaline phosphatase fusion proteins produced by one-step cloning as rapid detection tools for ELISA
    • 10.1081/IAS-120003657 10.1081/IAS-120003657 1:CAS:528: DC%2BD38XktFSmsbo%3D
    • Rau D, Kramer K, Hock B (2002) Single-chain Fv antibody-alkaline phosphatase fusion proteins produced by one-step cloning as rapid detection tools for ELISA. J Immunoassay Immunochem 23:129-143. doi: 10.1081/IAS-120003657
    • (2002) J Immunoassay Immunochem , vol.23 , pp. 129-143
    • Rau, D.1    Kramer, K.2    Hock, B.3
  • 16
    • 0242289399 scopus 로고    scopus 로고
    • Affibody-β-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol
    • 10.1016/j.jim.2003.06.001 10.1016/j.jim.2003.06.001
    • Rönnmark J, Kampf C, Asplund A, Höidén-Guthenberg I, Wester K, Pontén F, Uhlén M, Nygren PA (2003) Affibody-β- galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol. J Immunol Methods 281:149-160. doi: 10.1016/j.jim.2003.06.001
    • (2003) J Immunol Methods , vol.281 , pp. 149-160
    • Rönnmark, J.1    Kampf, C.2    Asplund, A.3    Höidén-Guthenberg, I.4    Wester, K.5    Pontén, F.6    Uhlén, M.7    Nygren, P.A.8
  • 17
    • 77956880053 scopus 로고    scopus 로고
    • Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells
    • 10.1016/j.jim.2010.07.012 10.1016/j.jim.2010.07.012 1:CAS:528: DC%2BC3cXhtFygu7zK
    • Sasajima Y, Iwasaki R, Tsumoto K, Kumagai I, Ihara M, Ueda H (2010) Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells. J Immunol Methods 361:57-63. doi: 10.1016/j.jim.2010.07.012
    • (2010) J Immunol Methods , vol.361 , pp. 57-63
    • Sasajima, Y.1    Iwasaki, R.2    Tsumoto, K.3    Kumagai, I.4    Ihara, M.5    Ueda, H.6
  • 18
    • 0034705337 scopus 로고    scopus 로고
    • A revised mechanism for the alkaline phosphatase reaction involving three metal ions
    • 10.1006/jmbi.2000.3799 10.1006/jmbi.2000.3799 1:CAS:528: DC%2BD3cXkt1Kgtb8%3D
    • Stec B, Holtz KM, Kantrowitz ER (2000) A revised mechanism for the alkaline phosphatase reaction involving three metal ions. J Mol Biol 299:1303-1311. doi: 10.1006/jmbi.2000.3799
    • (2000) J Mol Biol , vol.299 , pp. 1303-1311
    • Stec, B.1    Holtz, K.M.2    Kantrowitz, E.R.3
  • 19
    • 80051549218 scopus 로고    scopus 로고
    • Immunodiagnostic reagents using llama single domain antibody-alkaline phosphatase fusion proteins
    • 10.1016/j.ab.2011.06.012 10.1016/j.ab.2011.06.012 1:CAS:528: DC%2BC3MXpvFWgs78%3D
    • Swain MD, Anderson GP, Serrano-González J, Liu JL, Zabetakis D, Goldman ER (2011) Immunodiagnostic reagents using llama single domain antibody-alkaline phosphatase fusion proteins. Anal Biochem 417:188-194. doi: 10.1016/j.ab.2011.06.012
    • (2011) Anal Biochem , vol.417 , pp. 188-194
    • Swain, M.D.1    Anderson, G.P.2    Serrano-González, J.3    Liu, J.L.4    Zabetakis, D.5    Goldman, E.R.6
  • 20
    • 63149156774 scopus 로고    scopus 로고
    • PGreen-S: A clone vector bearing absence of enhanced green fluorescent protein for screening recombinants
    • 10.1016/j.ab.2009.02.007 10.1016/j.ab.2009.02.007 1:CAS:528: DC%2BD1MXktFamtro%3D
    • Tang J, Liang S, Zhang J, Gao Z, Zhang S (2009) pGreen-S: a clone vector bearing absence of enhanced green fluorescent protein for screening recombinants. Anal Biochem 388:173-174. doi: 10.1016/j.ab.2009.02.007
    • (2009) Anal Biochem , vol.388 , pp. 173-174
    • Tang, J.1    Liang, S.2    Zhang, J.3    Gao, Z.4    Zhang, S.5
  • 21
    • 0028222199 scopus 로고
    • A single Fc binding domain-alkaline phosphatase gene fusion expresses a protein with both IgG binding ability and alkaline phosphatase enzymatic activity
    • 10.1093/protein/7.5.715 10.1093/protein/7.5.715 1:CAS:528: DyaK2cXksFGgtbo%3D
    • Wang CL, Huang M, Wesson CA, Birdsell DC, Trumble WR (1994) A single Fc binding domain-alkaline phosphatase gene fusion expresses a protein with both IgG binding ability and alkaline phosphatase enzymatic activity. Protein Eng 7:715-722. doi: 10.1093/protein/7.5.715
    • (1994) Protein Eng , vol.7 , pp. 715-722
    • Wang, C.L.1    Huang, M.2    Wesson, C.A.3    Birdsell, D.C.4    Trumble, W.R.5
  • 22
    • 33144455961 scopus 로고    scopus 로고
    • Construction of single chain variable fragment (ScFv) and BiscFv-alkaline phosphatase fusion protein for detection of Bacillus anthracis
    • 10.1021/ac0512352 10.1021/ac0512352 1:CAS:528:DC%2BD28XitVGjsQ%3D%3D
    • Wang SH, Zhang JB, Zhang ZP, Zhou YF, Yang RF, Chen J, Guo YC, You F, Zhang XE (2006) Construction of single chain variable fragment (ScFv) and BiscFv-alkaline phosphatase fusion protein for detection of Bacillus anthracis. Anal Chem 78:997-1004. doi: 10.1021/ac0512352
    • (2006) Anal Chem , vol.78 , pp. 997-1004
    • Wang, S.H.1    Zhang, J.B.2    Zhang, Z.P.3    Zhou, Y.F.4    Yang, R.F.5    Chen, J.6    Guo, Y.C.7    You, F.8    Zhang, X.E.9
  • 23
    • 84872296215 scopus 로고
    • Antibody-enzyme conjugate formation
    • 10.1385/0-89603-126-8:373 1:STN:280:DC%2BC3M3mtlWgsg%3D%3D
    • Wisdom GB (1988) Antibody-enzyme conjugate formation. Methods Mol Biol 3:373-382. doi: 10.1385/0-89603-126-8:373
    • (1988) Methods Mol Biol , vol.3 , pp. 373-382
    • Wisdom, G.B.1
  • 24
    • 21644448479 scopus 로고    scopus 로고
    • Conjugation of antibodies to alkaline phosphatase
    • 10.1385/1-59259-873-0:123 1:CAS:528:DC%2BD2MXkt12rtr8%3D
    • Wisdom GB (2005) Conjugation of antibodies to alkaline phosphatase. Methods Mol Biol 295:123-126. doi: 10.1385/1-59259-873-0:123
    • (2005) Methods Mol Biol , vol.295 , pp. 123-126
    • Wisdom, G.B.1
  • 25
    • 0034660520 scopus 로고    scopus 로고
    • Genetically fused protein A-luciferase for immunological blotting analyses
    • 10.1006/abio.2000.4584 10.1006/abio.2000.4584 1:CAS:528: DC%2BD3cXktVGrtr8%3D
    • Zhang XM, Kobatake E, Kobayashi K, Yanagida Y, Aizawa M (2000) Genetically fused protein A-luciferase for immunological blotting analyses. Anal Biochem 282:65-69. doi: 10.1006/abio.2000.4584
    • (2000) Anal Biochem , vol.282 , pp. 65-69
    • Zhang, X.M.1    Kobatake, E.2    Kobayashi, K.3    Yanagida, Y.4    Aizawa, M.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.