Inhibition of Ephrin B3-mediated survival signaling contributes to increased cell death response of non-small cell lung carcinoma cells after combined treatment with ionizing radiation and PKC 412

Cell Death and Disease

Volumn 4, Issue 1, 2013, Pages

  Stahl S ^a   Kaminskyy, V O ^a   Efazat, G ^a   Hyrslova Vaculova, A ^a,b   Rodriguez Nieto, S ^a,c   Moshfegh, A ^a   Lewensohn, R ^a   Viktorsson, K ^a   Zhivotovsky, B ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b INSTITUTE OF BIOPHYSICS   (Czech Republic)

^c BELLVITGE BIOMEDICAL RESEARCH INSTITUTE IDIBELL   (Spain)

Author keywords

cell death and cell cycle arrest; Ephrin B3; gene array; NSCLC; radiation; senescence

Indexed keywords

EPHRIN B3; MIDOSTAURIN; MITOGEN ACTIVATED PROTEIN KINASE; SMALL INTERFERING RNA; SYNAPTOPHYSIN; CYCLIN DEPENDENT KINASE INHIBITOR 1B; DRUG DERIVATIVE; MITOGEN ACTIVATED PROTEIN KINASE P38; STAUROSPORINE;

ARTICLE; CANCER INHIBITION; CANCER RADIOTHERAPY; CELL PROLIFERATION; CELL STRUCTURE; CONTROLLED STUDY; DOWN REGULATION; ENZYME PHOSPHORYLATION; G2 PHASE CELL CYCLE CHECKPOINT; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENE SILENCING; HUMAN; HUMAN CELL; IONIZING RADIATION; LUNG NON SMALL CELL CANCER; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; TUMOR CELL DESTRUCTION; UPREGULATION; APOPTOSIS; DRUG ANTAGONISM; DRUG EFFECT; GENETICS; METABOLISM; MULTIMODALITY CANCER THERAPY; PATHOLOGY; PHOSPHORYLATION; RADIATION EXPOSURE; SIGNAL TRANSDUCTION; TUMOR CELL LINE;

APOPTOSIS; CARCINOMA, NON-SMALL-CELL LUNG; CELL LINE, TUMOR; COMBINED MODALITY THERAPY; CYCLIN-DEPENDENT KINASE INHIBITOR P27; EPHRIN-B3; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; G2 PHASE CELL CYCLE CHECKPOINTS; HUMANS; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHORYLATION; RADIATION, IONIZING; RNA INTERFERENCE; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; STAUROSPORINE; UP-REGULATION;

MLCS; MLOWN;

EID: 84872176286     PISSN: None     EISSN: 20414889     Source Type: Journal    
DOI: 10.1038/cddis.2012.188     Document Type: Article

References (32)

1. Viktorsson K, Lewensohn R. Apoptotic signaling pathways in lung cancer. J Thorac Oncol 2007; 2: 175-179. (Pubitemid 47181321)
2. Viktorsson K, Ekedahl J, Lindebro MC, Lewensohn R, Zhivotovsky B, Linder S et al. Defective stress kinase and Bak activation in response to ionizing radiation but not cisplatin in a non-small cell lung carcinoma cell line. Exp Cell Res 2003; 289: 256-264. (Pubitemid 37117411)
3. Joseph B, Marchetti P, Formstecher P, Kroemer G, Lewensohn R, Zhivotovsky B. Mitochondrial dysfunction is an essential step for killing of non-small cell lung carcinomas resistant to conventional treatment. Oncogene 2002; 21: 65-77. (Pubitemid 34076379)
4. Stahl S, Fung E, Adams C, Lengqvist J, Mork B, Stenerlow B et al. Proteomics and pathway analysis identifies JNK signaling as critical for high linear energy transfer radiation-induced apoptosis in non-small lung cancer cells. Mol Cell Proteomics 2009; 8: 1117-1129.
5. Hemstrom TH, Joseph B, Schulte G, Lewensohn R, Zhivotovsky B. PKC 412 sensitizes U1810 non-small cell lung cancer cells to DNA damage. Exp Cell Res 2005; 305: 200-213. (Pubitemid 40380666)
6. Hemstrom TH, Sandstrom M, Zhivotovsky B. Inhibitors of the PI3-kinase/Akt pathway induce mitotic catastrophe in non-small cell lung cancer cells. Int J Cancer 2006; 119: 1028-1038. (Pubitemid 44116909)
7. Rupa JD, de Bruine AP, Gerbers AJ, Leers MP, Nap M, Kessels AG et al. Simultaneous detection of apoptosis and proliferation in colorectal carcinoma by multiparameter flow cytometry allows separation of high and low-turnover tumors with distinct clinical outcome. Cancer 2003; 97: 2404-2411. (Pubitemid 36542478)
8. Hagg M, Biven K, Ueno T, Rydlander L, Bjorklund P, Wiman KG et al. A novel highthrough- put assay for screening of pro-Apoptotic drugs. Invest New Drugs 2002; 20: 253-259. (Pubitemid 34823753)
9. McCarron JK, Stringer BW, Day BW, Boyd AW. Ephrin expression and function in cancer. Future Oncol 2010; 6: 165-176.
10. Pasquale EB. Eph receptors and ephrins in cancer: bidirectional signalling and beyond. Nat Rev Cancer 2010; 10: 165-180.
11. Ding L, Getz G, Wheeler DA, Mardis ER, McLellan MD, Cibulskis K et al. Somatic mutations affect key pathways in lung adenocarcinoma. Nature 2008; 455: 1069-1075.
12. Alam SM, Fujimoto J, Jahan I, Sato E, Tamaya T. Coexpression of EphB4 and ephrinB2 in tumour advancement of ovarian cancers. Br J Cancer 2008; 98: 845-851. (Pubitemid 351272606)
13. Sos ML, Michel K, Zander T, Weiss J, Frommolt P, Peifer M et al. Predicting drug susceptibility of non-small cell lung cancers based on genetic lesions. J Clin Invest 2009; 119: 1727-1740.
14. Furne C, Ricard J, Cabrera JR, Pays L, Bethea JR, Mehlen P et al. EphrinB3 is an antiapoptotic ligand that inhibits the dependence receptor functions of EphA4 receptors during adult neurogenesis. Biochim Biophys Acta 2009; 1793: 231-238.
15. Quah BJ, Warren HS, Parish CR. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester. Nat Protoc 2007; 2: 2049-2056. (Pubitemid 351565842)
16. Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C et al. A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci USA 1995; 92: 9363-9367.
17. Stahl S, Branca RM, Efazat G, Ruzzene M, Zhivotovsky B, Lewensohn R et al. Phosphoproteomic profiling of NSCLC cells reveals that ephrin B3 regulates pro-survival signaling through Akt1-mediated phosphorylation of the EphA2 receptor. J Proteome Res 2011; 10: 2566-2578.
18. Brannan JM, Dong W, Prudkin L, Behrens C, Lotan R, Bekele BN et al. Expression of the receptor tyrosine kinase EphA2 is increased in smokers and predicts poor survival in nonsmall cell lung cancer. Clin Cancer Res 2009; 15: 4423-4430.
19. Brannan JM, Sen B, Saigal B, Prudkin L, Behrens C, Solis L et al. EphA2 in the early pathogenesis and progression of non-small cell lung cancer. Cancer Prev Res 2009; 2: 1039-1049.
20. Germain S, Eichmann A. VEGF and ephrin-B2: a bloody duo. Nat Med 2010; 16: 752-754.
21. Faoro L, Singleton PA, Cervantes GM, Lennon FE, Choong NW, Kanteti R et al. EphA2 mutation in lung squamous cell carcinoma promotes increased cell survival, cell invasion, focal adhesions, and mammalian target of rapamycin activation. J Biol Chem 2010; 285: 18575-18585.
22. Jonathan EC, Bernhard EJ, McKenna WG. How does radiation kill cells? Curr Opin Chem Biol 1999; 3: 77-83. (Pubitemid 29118984)
23. Vakifahmetoglu H, Olsson M, Tamm C, Heidari N, Orrenius S, Zhivotovsky B. DNA damage induces two distinct modes of cell death in ovarian carcinomas. Cell Death Differ 2008; 15: 555-566. (Pubitemid 351267306)
24. Kuilman T, Peeper DS. Senescence-messaging secretome: SMS-ing cellular stress. Nat Rev Cancer 2009; 9: 81-94.
25. Lin HK, Chen Z, Wang G, Nardella C, Lee SW, Chan CH et al. Skp2 targeting suppresses tumorigenesis by Arf-p53-independent cellular senescence. Nature 2010; 464: 374-379.
26. Sirzen F, Heiden T, Nilsson A, Bergh J, Skog S, Lewensohn R. Characterisation of the G1/S cell cycle checkpoint defect in lung carcinoma cells with different intrinsic radiosensitivities. Anticancer Res 1997; 17: 3381-3386. (Pubitemid 27522298)
27. Larrea MD, Hong F, Wander SA, da Silva TG, Helfman D, Lannigan D et al. RSK1 drives p27Kip1 phosphorylation at T198 to promote RhoA inhibition and increase cell motility. Proc Natl Acad Sci USA 2009; 106: 9268-9273.
28. Larrea MD, Wander SA, Slingerland JM. p27 as Jekyll and Hyde: regulation of cell cycle and cell motility. Cell Cycle 2009; 8: 3455-3461.
29. Bergh J, Nilsson K, Ekman R, Giovanella B. Establishment and characterization of cell lines from human small cell and large cell carcinomas of the lung. Acta Pathol Microbiol Immunol Scand A 1985; 93: 133-147. (Pubitemid 15061149)
30. Brodin O, Lennartsson L, Nilsson S. Single-dose and fractionated irradiation of four human lung cancer cell lines in vitro. Acta Oncol 1991; 30: 967-974.
31. Caulin C, Salvesen GS, Oshima RG. Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis. J Cell Biol 1997; 138: 1379-1394. (Pubitemid 27415063)
32. Nakada M, Drake KL, Nakada S, Niska JA, Berens ME. Ephrin-B3 ligand promotes glioma invasion through activation of Rac1. Cancer Res 2006; 66: 8492-8500. (Pubitemid 44449162)