Wnt signaling orchestration with a small molecule DYRK inhibitor provides long-term xeno-free human pluripotent cell expansion

Stem Cells Translational Medicine

Volumn 1, Issue 1, 2012, Pages 18-28

  Hasegawa, Kouichi ^a,b,c   Yasuda, Shin Ya ^a   Teo, Jia Ling ^a   Nguyen, Cu ^a   McMillan, Michael ^a   Hsieh, Chih Lin ^d   Suemori, Hirofumi ^e   Nakatsuji, Norio ^c,e   Yamamoto, Masashi ^f   Miyabayashi, Tomoyuki ^g   Lutzko, Carolyn ^b   Pera, Martin F ^a,b   Kahn, Michael ^a,b  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^c KYOTO UNIVERSITY   (Japan)

^d UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^e KYOTO UNIVERSITY   (Japan)

^f ASAHI KASEI CORPORATION   (Japan)

^g Asahi Kasei Medical Co   (United States)

Author keywords

DYRK; Human embryonic stem cells; Human induced pluripotent stem cells; Wnt

Indexed keywords

BETA CATENIN; DUAL SPECIFICITY TYROSINE PHOSPHORYLATION REGULATED KINASE; DUAL SPECIFICITY TYROSINE PHOSPHORYLATION REGULATED KINASE INHIBITOR; FIBROBLAST GROWTH FACTOR; ID 8; LENTIVIRUS VECTOR; PROTEIN TYROSINE KINASE; PROTEIN TYROSINE KINASE INHIBITOR; TRANSFORMING GROWTH FACTOR BETA; UNCLASSIFIED DRUG; DYRK KINASE; PROTEIN KINASE INHIBITOR; PROTEIN SERINE THREONINE KINASE; WNT3A PROTEIN;

ARTICLE; CELL ADHESION; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOPRECIPITATION; KARYOTYPE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PLURIPOTENT STEM CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTON NUCLEAR MAGNETIC RESONANCE; STEM CELL EXPANSION; WESTERN BLOTTING; WNT SIGNALING PATHWAY; ANIMAL; CELL LINE; CULTURE TECHNIQUE; DOSE RESPONSE; DRUG ANTAGONISM; DRUG EFFECT; EMBRYONIC STEM CELL; ENZYMOLOGY; GENE SILENCING; KARYOTYPING; METABOLISM; MOUSE; MOUSE MUTANT; RNA INTERFERENCE; TIME;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; DOSE-RESPONSE RELATIONSHIP, DRUG; EMBRYONIC STEM CELLS; GENE KNOCKDOWN TECHNIQUES; HUMANS; INDUCED PLURIPOTENT STEM CELLS; KARYOTYPING; MICE; MICE, SCID; PROTEIN KINASE INHIBITORS; PROTEIN-SERINE-THREONINE KINASES; PROTEIN-TYROSINE KINASES; RNA INTERFERENCE; TIME FACTORS; WNT SIGNALING PATHWAY; WNT3A PROTEIN;

EID: 84872006171     PISSN: 21576564     EISSN: 21576580     Source Type: Journal    
DOI: 10.5966/sctm.2011-0033     Document Type: Article

References (41)

1. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al. Embryonic stem cell lines derived from human blastocysts. Science 1998;282:1145-1147.
2. Reubinoff BE, Pera MF, Fong CY et al. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol 2000;18:399-404.
3. Yu J, Vodyanik MA, Smuga-Otto K et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007;318:1917-1920.
4. Takahashi K, Tanabe K, Ohnuki M et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007;131:861-872.
5. Ludwig TE, Levenstein ME, Jones JM et al. Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol 2006;24:185-187.
6. Lu J, Hou R, Booth CJ et al. Defined culture conditions of human embryonic stem cells. Proc Natl Acad Sci U S A 2006;103: 5688-5693.
7. Yao S, Chen S, Clark J et al. Long-term selfrenewal and directed differentiation of humanembryonic stem cells in chemically defined conditions. Proc Natl Acad Sc i U S A 2006;103: 6907-6912.
8. Pera MF, Tam PP. Extrinsic regulation of pluripotent stem cells. Nature 2010;465:713-720.
9. Hasegawa K, Pomeroy JE, Pera MF. Current technology for the derivation of pluripotent stem cell lines from human embryos. Cell Stem Cell 2010;6:521-531.
10. Chen G, Gulbranson DR, Hou Z et al. Chemically defined conditions for human iPSC derivation and culture. Nat Methods 2011;8: 424-429.
11. Komiya Y, Habas R. Wnt signal transduction pathways. Organogenesis 2008;4:68-75.
12. Sato N, Meijer L, Skaltsounis L et al. Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med 2004;10: 55-63.
13. Dravid G, Ye Z, Hammond H et al. Defining the role of Wnt/-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells. STEM CELLS 2005;23: 1489-1501.
14. Lyashenko N, Winter M, Migliorini D et al. Differential requirement for the dual functions of beta-catenin in embryonic stem cell self-renewal and germ layer formation. Nat Cell Biol 2011;13:753-761.
15. Wray J, Kalkan T, Gomez-Lopez S et al. Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation. Nat Cell Biol 2011;13: 838-845.
16. Yi F, Pereira L, Hoffman JA et al. Opposing effects of Tcf3 and Tcf1 control Wnt stimulation of embryonic stem cell self-renewal. Nat Cell Biol 2011;13:762-770.
17. Cai L, Ye Z, Zhou BY et al. Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions. Cell Res 2007;17:62-72.
18. Melchior K, Weiß J, Zaehres H et al. The WNT receptor FZD7 contributes to self-renewal signaling of human embryonic stem cells. Biol Chem 2008;389:897-903.
19. Sumi T, Tsuneyoshi N, Nakatsuji N et al. Defining early lineage specification of human embryonic stem cells by the orchestrated balance of canonical Wnt/-catenin, Activin/ Nodal and BMP signaling. Development 2008; 135:2969-2979.
20. Otero JJ, Fu W, Kan L et al. Catenin signaling is required for neural differentiation of embryonic stem cells. Development 2004; 131:3545-3557.
21. Emami KH, Nguyen C, Ma H et al. A small molecule inhibitor of beta-catenin/ CREB-binding protein transcription [corrected]. Proc Natl Acad Sc i U S A 2004;101: 12682-12687.
22. Miyabayashi T, Teo J-L, Yamamoto M et al. Wnt/-catenin/CBP signaling maintains long-term murine embryonic stem cell pluripotency. Proc Natl Acad Sc i U S A 2007;104:5668-5673.
23. Lukaszewicz AI, McMillan MK, Kahn M. Small molecules and stem cells. Potency and lineage commitment: The new quest for the fountain of youth. J Med Chem 2010;53:3439-3453.
24. Miyabayashi T, Yamamoto M, Sato A et al. Indole derivatives sustain embryonic stem cell self-renewal in long-term culture. Biosci Biotechnol Biochem 2008;72:1242-1248.
25. Hasegawa K, Zhang P, Wei Z et al. Comparison of reprogramming efficiency between transduction of reprogramming factors, cell-cell fusion, and cytoplast fusion. STEM CELLS 2010;28:1338-1348.
26. Hasegawa K, Fujioka T, Nakamura Y et al. A method for the selection of human embryonic stem cell sublines with high replating efficiency after single-cell dissociation. STEM CELLS 2006;24:2649-2660.
27. Willert K, Brown JD, Danenberg E et al. Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature 2003;423:448-452.
28. Veeman MT, Slusarski DC, Kaykas A et al. Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Curr Biol 2003;13:680-685.
29. Xu C, Inokuma MS, Denham J et al. Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 2001;19: 971-974.
30. Watanabe K, Ueno M, Kamiya D et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 2007;25:681-686.
31. Chung TL, Turner JP, Thaker NY et al. Ascorbate promotes epigenetic activation of CD30 in human embryonic stem cells. STEM CELLS 2010;28:1782-1793.
32. Ellerström C, Strehl R, Moya K et al. Derivation of a xeno-free human embryonic stem cell line. STEM CELLS 2006;24:2170-2176.
33. Crook JM, Peura TT, Kravets L et al. The generation of six clinical-grade human embryonic stem cell lines. Cell Stem Cell 2007;1:490-494.
34. Desbordes SC, Placantonakis DG, Ciro A et al. High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Cell Stem Cell 2008;2: 602-612.
35. Damoiseaux R, Sherman SP, Alva JA et al. Integrated chemical genomics reveals modifiers of survival in human embryonic stem cells. STEM CELLS 2009;27:533-542.
36. Kelly KF, Ng DY, Jayakumaran G et al. Catenin enhances Oct-4 activity and reinforces pluripotency through a TCF-independent mechanism. Cell Stem Cell 2011;8:214-227.
37. Berge DT, Kurek D, Blauwkamp T et al. Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells. Nat Cell Biol 2011;13:1070-1075.
38. Mercer SE, Friedman E. Mirk/Dyrk1B: a multifunctional dual-specificity kinase involved in growth arrest, differentiation, and cell survival. Cell Biochem Biophys 2006;45: 303-315.
39. Takahashi-Yanaga F, Sasaguri T. GSK3beta regulates cyclin D1 expression: a new target for chemotherapy. Cell Signal 2008;20: 581-589.
40. Park J, Song W-J, Chung KC. Function and regulation of Dyrk1A: towards understanding Down syndrome. Cell Mol Life Sci 2009;66: 3235-3240.
41. Marson A, Foreman R, Chevalier B et al. Wnt signaling promotes reprogramming of somatic cells to pluripotency. Cell Stem Cell 2008; 3:132-135.