A zinc finger nuclease assay to rapidly quantitate homologous recombination proficiency in human cell lines

Analytical Biochemistry

Volumn 434, Issue 1, 2013, Pages 96-98

  Yuan, Jing ^a   Strack, Peter R ^a   Toniatti, Carlo ^a   Pelletier, Marc ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Colony formation assay; Homologous recombination; PCR; Zinc finger nuclease

Indexed keywords

CELL CULTURE; POLYMERASE CHAIN REACTION; ZINC;

CANCER THERAPEUTICS; CELLULAR MECHANISMS; COLONY FORMATION; DOUBLE-STRAND DNAS; HOMOLOGOUS RECOMBINATION; HUMAN CELL LINES; INDUCED ACTIVITY; ZINC FINGER NUCLEASE;

DISEASES;

BRCA1 PROTEIN; BRCA2 PROTEIN; GENOMIC DNA; SHORT HAIRPIN RNA; ZINC FINGER NUCLEASE;

AMPLICON; ARTICLE; BINDING SITE; CANCER CELL CULTURE; COLONY FORMATION; CONTROLLED STUDY; COPY NUMBER VARIATION; DELETION MUTANT; DOUBLE STRANDED DNA BREAK; ENZYME ASSAY; GENE LOCUS; GENE SILENCING; GENETIC TRANSFECTION; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; LOSS OF FUNCTION MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 84871998482     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.11.002     Document Type: Article

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