Homologous Recombination Assay for Interstrand Cross-Link Repair

Methods in Molecular Biology

Volumn 745, Issue , 2011, Pages 283-291

  Nakanishi, Koji ^a   Cavallo, Francesca ^b   Brunet, Erika ^c   Jasin, Maria ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

^c MUSÉUM NATIONAL D'HISTOIRE NATURELLE   (France)

Author keywords

GFP reporters; Homologous recombination; interstrand cross link repair; triplex forming oligonucleotide

Indexed keywords

OLIGONUCLEOTIDE;

ARTICLE; DNA REPAIR; FLOW CYTOMETRY; GENETIC RECOMBINATION; GENETICS; HUMAN; PLASMID; TUMOR CELL LINE;

CELL LINE, TUMOR; DNA REPAIR; FLOW CYTOMETRY; HUMANS; OLIGONUCLEOTIDES; PLASMIDS; RECOMBINATION, GENETIC;

EID: 80053333958     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-129-1_16     Document Type: Chapter

References (23)

1. Guainazzi, A., and Schärer, O.D. (2010) Using synthetic DNA interstrand crosslinks to elucidate repair pathways and identify new therapeutic targets for cancer chemotherapy. Cell Mol Life Sci 67, 3683–3697.
2. Hinz, J.M. (2010) Role of homologous recombination in DNA interstrand crosslink repair. Environ Mol Mutagen 51, 582–603.
3. Moynahan, M.E., Chiu, J.W., Koller, B.H., and Jasin, M. (1999) Brca1 controls homology-directed DNA repair. Mol Cell 4, 511–518.
4. Moynahan, M.E., Cui, T.Y., and Jasin, M. (2001) Homology-directed DNA repair, mitomycin-c resistance, and chromosome stability is restored with correction of a Brca1 mutation. Cancer Res 61, 4842–4850.
5. Moynahan, M.E., Pierce, A.J., and Jasin, M. (2001) BRCA2 is required for homology-directed repair of chromosomal breaks. Mol Cell 7, 263–272.
6. Kraakman-van der Zwet, M., et al. (2002) Brca2 (XRCC11) deficiency results in radioresistant DNA synthesis and a higher frequency of spontaneous deletions. Mol Cell Biol 22, 669–679.
7. Wang, W. (2007) Emergence of a DNA-damage response network consisting of Fanconi anaemia and BRCA proteins. Nat Rev Genet 8, 735–748.
8. Auerbach, A.D. (2009) Fanconi anemia and its diagnosis. Mutat Res 668, 4–10.
9. Nakanishi, K., et al. (2005) Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair. Proc Natl Acad Sci USA 102, 1110–1115.
10. Nakanishi, K., Cavallo, F., Perrouault, L., Giovannangeli, C., Moynahan, M.E., Barchi, M., Brunet, E., and Jasin, M. (2011) Homology-directed Fanconi anemia pathway cross-link repair is dependent on DNA replication. Nat Struct Mol Biol doi:10.1038/nsmb.2029.
11. Pierce, A.J., Johnson, R.D., Thompson, L.H., and Jasin, M. (1999) XRCC3 promotes homology-directed repair of DNA damage in mammalian cells. Genes Dev 13, 2633–2638.
12. Pierce, A.J., and Jasin, M. (2005) Measuring recombination proficiency in mouse embryonic stem cells. Methods Mol Biol 291, 373–384.
13. Moynahan, M.E., and Jasin, M. (2010) Mitotic homologous recombination maintains genomic stability and suppresses tumorigenesis. Nat Rev Mol Cell Biol 11, 196–207.
14. Slabicki, M., et al. (2010) A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. PLoS Biol 8, e1000408.
15. Chin, J.Y., and Glazer, P.M. (2009) Repair of DNA lesions associated with triplex-forming oligonucleotides. Mol Carcinog 48, 389–399.
16. Raha, M., Wang, G., Seidman, M.M., and Glazer, P.M. (1996) Mutagenesis by third-strand-directed psoralen adducts in repair-deficient human cells: high frequency and altered spectrum in a xeroderma pigmentosum variant. Proc Natl Acad Sci USA 93, 2941–2946.
17. Reisman, D., Yates, J., and Sugden, B. (1985) A putative origin of replication of plasmids derived from Epstein-Barr virus is composed of two cis-acting components. Mol Cell Biol 5, 1822–1832.
18. Pierce, A.J., Hu, P., Han, M., Ellis, N., and Jasin, M. (2001) Ku DNA end-binding protein modulates homologous repair of double-strand breaks in mammalian cells. Genes Dev 15, 3237–3242.
19. Richardson, C., Moynahan, M.E., and Jasin, M. (1998) Double-strand break repair by interchromosomal recombination: suppression of chromosomal translocations. Genes Dev 12, 3831–3842.
20. Niwa, H., Yamamura, K., and Miyazaki, J. (1991) Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108, 193–199.
21. Brunet, E., et al. (2005) Exploring cellular activity of locked nucleic acid-modified triplex-forming oligonucleotides and defining its molecular basis. J Biol Chem 280, 20076–20085.
22. Brunet, E., Corgnali, M., Cannata, F., Perrouault, L., and Giovannangeli, C. (2006) Targeting chromosomal sites with locked nucleic acid-modified triplex-forming oligonucleotides: study of efficiency dependence on DNA nuclear environment. Nucleic Acids Res 34, 4546–4553.
23. Raschle, M., et al. (2008) Mechanism of replication-coupled DNA interstrand crosslink repair. Cell 134, 969–980.