Cra regulates the cross-talk between the two branches of the phosphoenolpyruvate: Phosphotransferase system of Pseudomonas putida

Environmental Microbiology

Volumn 15, Issue 1, 2013, Pages 121-132

  Chavarría, Max ^a,b   Fuhrer, Tobias ^c   Sauer, Uwe ^c   Pflüger Grau, Katharina ^a,d   De Lorenzo, Víctor ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

^b UNIVERSIDAD DE COSTA RICA   (Costa Rica)

^c ETH ZURICH   (Switzerland)

^d TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; FRUCTOSE; FRUR PROTEIN, BACTERIA; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE; REPRESSOR PROTEIN;

ARTICLE; ENZYMOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; INTRACELLULAR SPACE; METABOLISM; OPERON; PHOSPHORYLATION; PSEUDOMONAS PUTIDA;

BACTERIAL PROTEINS; FRUCTOSE; INTRACELLULAR SPACE; OPERON; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE SYSTEM; PHOSPHORYLATION; PSEUDOMONAS PUTIDA; REPRESSOR PROTEINS;

PSEUDOMONAS PUTIDA;

EID: 84871936294     PISSN: 14622912     EISSN: 14622920     Source Type: Journal    
DOI: 10.1111/j.1462-2920.2012.02808.x     Document Type: Article

References (58)

1. Bledig, S.A., Ramseier, T.M., and Saier, M.H., Jr (1996) FruR mediates catabolite activation of pyruvate kinase (pykF) gene expression in Escherichia coli. J Bacteriol 178: 280-283.
2. Boel, G., Mijakovic, I., Maze, A., Poncet, S., Taha, M.K., Larribe, M., etal. (2003) Transcription regulators potentially controlled by HPr kinase/phosphorylase in Gram-negative bacteria. J Mol Microbiol Biotechnol 5: 206-215.
3. Buescher, J.M., Moco, S., Sauer, U., and Zamboni, N. (2010) Ultrahigh performance liquid chromatography-tandem mass spectrometry method for fast and robust quantification of anionic and aromatic metabolites. Anal Chem 82: 4403-4412.
4. Ntr (PtsN) protein of Pseudomonas putida mediates the C source inhibition of the sigma 54-dependent Pu promoter of the TOL plasmid. J Biol Chem 274: 15562-15568.
5. Ntr) protein of Pseudomonas putida. J Bacteriol 183: 1032-1037.
6. Cases, I., Velazquez, F., and de Lorenzo, V. (2001b) Role of ptsO in carbon-mediated inhibition of the Pu promoter belonging to the pWW0 Pseudomonas putida plasmid. J Bacteriol 183: 5128-5133.
7. Cases, I., Velazquez, F., and de Lorenzo, V. (2007) The ancestral role of the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) as exposed by comparative genomics. Res Microbiol 158: 666-670.
8. Chavarria, M., Santiago, C., Platero, R., Krell, T., Casasnovas, J.M., and de Lorenzo, V. (2011) Fructose 1-Phosphate is the preferred effector of the metabolic regulator Cra of Pseudomonas putida. J Biol Chem 286: 9351-9359.
9. Chavarria, M., Kleijn, R.J., Sauer, U., Pflüger-Grau, K., and de Lorenzo, V. (2012) Regulatory tasks of the phosphoenolpyruvate-phosphotransferase system of Pseudomonas putida in central carbon metabolism. mBio 3: e00028-e00012.
10. Ntr-SsrB interaction is required for Salmonella virulence. Proc Natl Acad Sci USA 107: 20506-20511.
11. Cortay, J.C., Negre, D., Scarabel, M., Ramseier, T.M., Vartak, N.B., Reizer, J., etal. (1994) In vitro asymmetric binding of the pleiotropic regulatory protein, FruR, to the ace operator controlling glyoxylate shunt enzyme synthesis. J Biol Chem 269: 14885-14891.
12. Deutscher, J., Francke, C., and Postma, P.W. (2006) How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria. Microbiol Mol Biol Rev 70: 939-1031.
13. Fuhrer, T., and Sauer, U. (2009) Different biochemical mechanisms ensure network-wide balancing of reducing equivalents in microbial metabolism. J Bacteriol 191: 2112-2121.
14. Horton, R.M., Hunt, H.D., Ho, S.N., Pullen, J.K., and Pease, L.R. (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77: 61-68.
15. Jurado, P., Ritz, D., Beckwith, J., de Lorenzo, V., and Fernandez, L.A. (2002) Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli. J Mol Biol 320: 1-10.
16. Kundig, W., Ghosh, S., and Roseman, S. (1964) Phosphate bound to histidine in a protein as an intermediate in a novel phospho-transferase system. Proc Natl Acad Sci USA 52: 1067-1074.
17. + transporter TrkA. Proc Natl Acad Sci USA 104: 4124-4129.
18. Ntr-dependent regulation of sigma factor selectivity. Mol Microbiol 78: 1468-1483.
19. Lengeler, J.W., and Jahreis, K. (2009) Bacterial PEP-dependent carbohydrate: phosphotransferase systems couple sensing and global control mechanisms. Contrib Microbiol 16: 65-87.
20. de Lorenzo, V., Herrero, M., Jakubzik, U., and Timmis, K.N. (1990) Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J Bacteriol 172: 6568-6572.
21. Ntr in Escherichia coli. Mol Microbiol 72: 978-994.
22. Martinez-Garcia, E., and de Lorenzo, V. (2011) Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440. Environ Microbiol 13: 2702-2716.
23. Miller, J.H. (1972) Experiments in Molecular Genetics. New York, NY, USA: Cold Spring Harbor Press.
24. Mitrophanov, A.Y., and Groisman, E.A. (2008) Signal integration in bacterial two-component regulatory systems. Genes Dev 22: 2601-2611.
25. Negre, D., Oudot, C., Prost, J.F., Murakami, K., Ishihama, A., Cozzone, A.J., and Cortay, J.C. (1998) FruR-mediated transcriptional activation at the ppsA promoter of Escherichia coli. J Mol Biol 276: 355-365.
26. Nelson, K.E., Weinel, C., Paulsen, I.T., Dodson, R.J., Hilbert, H., Martins dos Santos, V.A., etal. (2002) Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environ Microbiol 4: 799-808.
27. Ninfa, A.J. (2011) Unnecessary signaling: poorly named? J Bacteriol 193: 4571-4573.
28. Nogales, J., Palsson, B.O., and Thiele, I. (2008) A genome-scale metabolic reconstruction of Pseudomonas putida KT2440: iJN746 as a cell factory. BMC Syst Biol 2:79.
29. Ow, D.S., Lee, R.M., Nissom, P.M., Philp, R., Oh, S.K., and Yap, M.G. (2007) Inactivating FruR global regulator in plasmid-bearing Escherichia coli alters metabolic gene expression and improves growth rate. J Biotechnol 131: 261-269.
30. Ntr) protein of Pseudomonas putida. J Biol Chem 282: 18206-18211.
31. Pflüger, K., and de Lorenzo, V. (2008) Evidence of in vivo cross talk between the nitrogen-related and fructose-related branches of the carbohydrate phosphotransferase system of Pseudomonas putida. J Bacteriol 190: 3374-3380.
32. Pflüger, K., di Bartolo, I., Velazquez, F., and de Lorenzo, V. (2007) Non-disruptive release of Pseudomonas putida proteins by in situ electric breakdown of intact cells. J Microbiol Methods 71: 179-185.
33. Pflüger-Grau, K., and Görke, B. (2010) Regulatory roles of the bacterial nitrogen-related phosphotransferase system. Trends Microbiol 18: 205-214.
34. Ntr) of Pseudomonas putida with pyruvate dehydrogenase. Biochim Biophys Acta 1810: 995-1005.
35. Postma, P.W., Lengeler, J.W., and Jacobson, G.R. (1993) Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol Rev 57: 543-594.
36. ts mutant. J Biol Chem 270: 4822-4839.
37. Prost, J.F., Negre, D., Oudot, C., Murakami, K., Ishihama, A., Cozzone, A.J., and Cortay, J.C. (1999) Cra-dependent transcriptional activation of the icd gene of Escherichia coli. J Bacteriol 181: 893-898.
38. Puchalka, J., Oberhardt, M.A., Godinho, M., Bielecka, A., Regenhardt, D., Timmis, K.N., etal. (2008) Genome-scale reconstruction and analysis of the Pseudomonas putida KT2440 metabolic network facilitates applications in biotechnology. PLoS Comput Biol 4: e1000210.
39. Ntr from Escherichia coli. A novel enzyme of the phosphoenolpyruvate-dependent phosphotransferase system exhibiting strict specificity for its phosphoryl acceptor, NPr. J Biol Chem 274: 26185-26191.
40. Ramseier, T.M. (1996) Cra and the control of carbon flux via metabolic pathways. Res Microbiol 147: 489-493.
41. Ramseier, T.M., Negre, D., Cortay, J.C., Scarabel, M., Cozzone, A.J., and Saier, M.H., Jr (1993) In vitro binding of the pleiotropic transcriptional regulatory protein, FruR, to the fru, pps, ace, pts and icd operons of Escherichia coli and Salmonella typhimurium. J Mol Biol 234: 28-44.
42. Ramseier, T.M., Bledig, S., Michotey, V., Feghali, R., and Saier, M.H., Jr (1995) The global regulatory protein FruR modulates the direction of carbon flow in Escherichia coli. Mol Microbiol 16: 1157-1169.
43. Raveh, H., Lopian, L., Nussbaum-Shochat, A., Wright, A., and Amster-Choder, O. (2009) Modulation of transcription antitermination in the bgl operon of Escherichia coli by the PTS. Proc Natl Acad Sci USA 106: 13523-13528.
44. Reaves, M.L., and Rabinowitz, J.D. (2011) Characteristic phenotypes associated with ptsN-null mutants in Escherichia coli K-12 are absent in strains with functional ilvG. J Bacteriol 193: 4576-4581.
45. Reizer, J., Reizer, A., Saier, M.H., Jr, and Jacobson, G.R. (1992a) A proposed link between nitrogen and carbon metabolism involving protein phosphorylation in bacteria. Protein Sci 1: 722-726.
46. Reizer, J., Sutrina, S.L., Wu, L.F., Deutscher, J., Reddy, P., and Saier, M.H. (1992b) Functional interactions between proteins of the phosphoenolpyruvate:sugar phosphotransferase systems of Bacillus subtilis and Escherichia coli. J Biol Chem 267: 9158-9169.
47. Saier, M.H., Jr, and Ramseier, T.M. (1996) The catabolite repressor/activator (Cra) protein of enteric bacteria. J Bacteriol 178: 3411-3417.
48. Sambrook, J., Maniatis, T., and Fritsch, T. (1989) Molecular Cloning: A Laboratory Manual. New York, NY, USA: Cold Spring Harbor Press.
49. Sarkar, D., Siddiquee, K.A., Arauzo-Bravo, M.J., Oba, T., and Shimizu, K. (2008) Effect of cra gene knockout together with edd and iclR genes knockout on the metabolism in Escherichia coli. Arch Microbiol 190: 559-571.
50. Schultz, J.E. (2009) Structural and biochemical aspects of tandem GAF domains. Handb Exp Pharmacol 191: 93-109.
51. Silva-Rocha, R., and de Lorenzo, V. (2010) Noise and robustness in prokaryotic regulatory networks. Ann Rev Microbiol 64: 257-275.
52. Siryaporn, A., and Goulian, M. (2008) Cross-talk suppression between the CpxA-CpxR and EnvZ-OmpR two-component systems in E. coli. Mol Microbiol 70: 494-506.
53. Stock, A.M., Robinson, V.L., and Goudreau, P.N. (2000) Two-component signal transduction. Annu Rev Biochem 69: 183-215.
54. Sutrina, S.L., Reddy, P., Saier, M.H., and Reizer, J.J. (1990) The glucose permease of Bacillus subtilis is a single polypeptide chain that functions to energize the sucrose permease. J Biol Chem 265: 18581-18589.
55. Velazquez, F., Pflüger, K., Cases, I., De Eugenio, L.I., and de Lorenzo, V. (2007) The phosphotransferase system formed by PtsP, PtsO, and PtsN proteins controls production of polyhydroxyalkanoates in Pseudomonas putida. J Bacteriol 189: 4529-4533.
56. Verhamme, D.T., Arents, J.C., Postma, P.W., Crielaard, W., and Hellingwerf, K.J. (2002) Investigation of in vivo cross-talk between key two-component systems of Escherichia coli. Microbiology 148: 69-78.
57. Yuste, L., Hervas, A.B., Canosa, I., Tobes, R., Jimenez, J.I., Nogales, J., etal. (2006) Growth phase-dependent expression of the Pseudomonas putida KT2440 transcriptional machinery analysed with a genome-wide DNA microarray. Environ Microbiol 8: 165-177.
58. Ntr protein in vivo. FEMS Microbiol Lett 286: 96-102.