P53 and cell cycle effects after DNA damage

Methods in Molecular Biology

Volumn 962, Issue , 2013, Pages 49-61

  Senturk, Emir ^a   Manfredi, James J ^b  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^b Ichan Medical Institute   (United States)

Author keywords

Apoptosis; BrdU; DNA damage; DNA damage checkpoints; Dual parameter; Flow cytometry; Fluorescence; G1 arrest; G2 M arrest; p53; Phosho histone H3; Propidium iodide

Indexed keywords

EID: 84871863296     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-236-0_4     Document Type: Article

References (19)

1. Hartwell LH, Weinert TA (1989) Checkpoints: controls that ensure the order of cell cycle events. Science 246:629-634
2. Paulovich AG, Toczyski DP, Hartwell LH (1997) When checkpoints fail. Cell 88: 315-321
3. Kern SE, Kinzler KW, Bruskin A, Jarosz D, Friedman P, Prives C, Vogelstein B (1991) Identification of p53 as a sequence-specific DNA-binding protein. Science 252: 1708-1711
4. Pietenpol JA, Tokino T, Thiagalingam S, el-Deiry WS, Kinzler KW, Vogelstein B (1994) Sequence-specific transcriptional activation is essential for growth suppression by p53. Proc Natl Acad Sci USA 91:1998-2002
5. Finlay CA, Hinds PW, Levine AJ (1989) The p53 proto-oncogene can act as a suppressor of transformation. Cell 57:1083-1093
6. Burns TF, El-Deiry WS (1999) The p53 pathway and apoptosis. J Cell Physiol 181: 231-239
7. Bargonetti J, Manfredi JJ (2002) Multiple roles of the tumor suppressor p53. Curr Opin Oncol 14:86-91
8. el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein B (1993) WAF1, a potential mediator of p53 tumor suppression. Cell 75:817-825
9. Harper JW, Adami GR, Wei N, Keyomarsi K, Elledge SJ (1993) The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75:805-816
10. Waldman T, Kinzler KW, Vogelstein B (1995) p21 is necessary for the p53-mediated G1 arrest in human cancer cells. Cancer Res 55:5187-5190
11. Waga S, Hannon GJ, Beach D, Stillman B (1994) The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interac tion with PCNA. Nature 369:574-578
12. Agarwal ML, Agarwal A, Taylor WR, Stark GR (1995) p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates revers ible growth arrest in humanfibroblasts. Proc Natl Acad Sci USA 92:8493-8497
13. Flatt PM, Tang LJ, Scatena CD, Szak ST, Pietenpol JA (2000) p53 regulation of G(2) checkpoint is retinoblastoma protein dependent. Mol Cell Biol 20:4210-4223
14. Matsuoka S, Huang M, Elledge SJ (1998) Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 282:1893-1897
15. Taylor WR, DePrimo SE, Agarwal A, Agarwal ML, Schonthal AH, Katula KS, Stark GR (1999) Mechanisms of G2 arrest in response to overexpression of p53. Mol Biol Cell 10: 3607-3622
16. Bunz F, Dutriaux A, Lengauer C, Waldman T, Zhou S, Brown JP, Sedivy JM, Kinzler KW, Vogelstein B (1998) Requirement for p53 and p21 to sustain G2 arrest after DNA damage. Science 282:1497-1501
17. Chan TA, Hermeking H, Lengauer C, Kinzler KW, Vogelstein B (1999) 14-3-3Sigma is required to prevent mitotic catastrophe after DNA damage. Nature 401:616-620
18. Kastan MB, Zhan Q, el-Deiry WS, Carrier F, Jacks T, Walsh WV, Plunkett BS, Vogelstein B, Fornace AJ Jr (1992) A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia. Cell 71: 587-597
19. Malkin D (1993) p53 and the Li-Fraumeni syndrome. Cancer Genet Cytogenet 66:83-92