Predictive models for the accumulation of a fluorescent marker protein in tobacco leaves according to the promoter/5′UTR combination

Biotechnology and Bioengineering

Volumn 110, Issue 2, 2013, Pages 471-482

  Buyel, J F ^a   Kaever, T ^b   Buyel, J J ^b   Fischer, R ^a,b  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

Author keywords

5 UTR; Balanced expression; Design of experiments; Predictive model; Promoter strength; Recombinant protein; Transient expression

Indexed keywords

35S PROMOTER; AGROBACTERIUM TUMEFACIENS; BALANCED EXPRESSION; CAULIFLOWER MOSAIC VIRUS; COMPARATIVE EXPERIMENTS; EXPRESSION LEVELS; FLUORESCENT MARKERS; INCUBATION PERIODS; INCUBATION TIME; LEAF POSITION; MARKER PROTEINS; MODEL PREDICTION; PREDICTIVE MODELS; PROMOTER STRENGTH; PROTEIN ACCUMULATION; PROTEIN TARGETING; PROTEIN YIELD; RAPID IDENTIFICATION; RECOMBINANT PROTEIN EXPRESSION; SPATIO-TEMPORAL VARIATION; TOBACCO LEAVE; TOBACCO PLANTS; TRANSGENIC PLANTS; TRANSIENT EXPRESSION; UNTRANSLATED REGIONS; WHOLE PLANTS;

DESIGN OF EXPERIMENTS; FLUORESCENCE; RECOMBINANT PROTEINS; TOBACCO; VIRUSES;

PLANTS (BOTANY);

CYAN FLUORESCENT PROTEIN; DSRED PROTEIN; UNCLASSIFIED DRUG;

5' UNTRANSLATED REGION; ARTICLE; CAULIFLOWER MOSAIC VIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT LEAF; PREDICTION; PROTEIN EXPRESSION; RHIZOBIUM RADIOBACTER; TOBACCO;

5' UNTRANSLATED REGIONS; AGROBACTERIUM TUMEFACIENS; CAULIMOVIRUS; ENDOPLASMIC RETICULUM; FLUORESCENT DYES; GENE EXPRESSION REGULATION, PLANT; LUMINESCENT PROTEINS; MODELS, STATISTICAL; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; PROMOTER REGIONS, GENETIC; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; THERMODYNAMICS; TIME FACTORS; TOBACCO;

AGROBACTERIUM TUMEFACIENS; CAULIFLOWER MOSAIC VIRUS; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 84871833437     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24715     Document Type: Article

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