Recombinant haemagglutinin protein of highly pathogenic avian influenza A (H5N1) virus expressed in Pichia pastoris elicits a neutralizing antibody response in mice

Journal of Virological Methods

Volumn 187, Issue 1, 2013, Pages 20-25

  Murugan, Subathra ^a   Ponsekaran, Santhakumar ^b   Kannivel, Lingaraja ^b   Mangamoori, Lakshmi Narasu ^a   Chandran, Dev ^b   Villuppanoor Alwar, Srinivasan ^b   Chakravarty, Chandrani ^c   Lal, Sunil K ^c  

^a JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY   (India)

^b Indian Immunologicals Limited   (India)

^c INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Italy)

Author keywords

Enzyme linked immunosorbent assay; Haemaglutination; Haemaglutination inhibition; Highly pathogenic avian influenza; Immunogenicity

Indexed keywords

AVIAN INFLUENZA VACCINE; INFLUENZA VIRUS HEMAGGLUTININ; NEUTRALIZING ANTIBODY; RECOMBINANT INFLUENZA VIRUS HEMAGGLUTININ; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VIRUS ANTIBODY;

AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ANTIBODY RESPONSE; ANTIBODY TITER; ANTIGEN SPECIFICITY; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENE EXPRESSION; HEMAGGLUTINATION INHIBITION TEST; HEMAGGLUTININ GENE; IMMUNE RESPONSE; IMMUNOGENICITY; INFLUENZA VIRUS A H5N1; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN REFOLDING; VIRUS GENE; VIRUS VIRULENCE; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, NEUTRALIZING; ANTIBODIES, VIRAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; GENE EXPRESSION; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; INFLUENZA A VIRUS, H5N1 SUBTYPE; INFLUENZA IN BIRDS; INFLUENZA VACCINES; MICE; PICHIA; POULTRY; RECOMBINANT PROTEINS; VACCINATION; VACCINES, SYNTHETIC;

AVES; INFLUENZA A VIRUS; MUS; PICHIA PASTORIS;

EID: 84871797768     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.07.026     Document Type: Article

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