Expression, purification and characterization of low-glycosylation influenza neuraminidase in α-1,6-mannosyltransferase defective Pichia pastoris

Molecular Biology Reports

Volumn 39, Issue 2, 2012, Pages 857-864

  Yang, Yi Li ^a   Chang, Shao Hong ^a   Gong, Xin ^a   Wu, Jun ^a   Liu, Bo ^a  

^a BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

Author keywords

1,6 Mannosyltransferase; Glycosylation; Immunogenicity; Neuraminidase; Pichia pastoris

Indexed keywords

ALPHA 1,6 MANNOSYLTRANSFERASE; HN PROTEIN; MANNOSYLTRANSFERASE; PROTEIN OCH1; UNCLASSIFIED DRUG; VIRUS SIALIDASE; INFLUENZA VACCINE; MEMBRANE PROTEIN; PRIMER DNA; RECOMBINANT PROTEIN; SIALIDASE; VIRUS ANTIBODY;

ANIMAL EXPERIMENT; ANTIBODY TITER; ARTICLE; CONTROLLED STUDY; DEGLYCOSYLATION; DOSE RESPONSE; ESCHERICHIA COLI; EXPRESSION VECTOR; FEMALE; FUNGAL STRAIN; GLYCOSYLATION; IMMUNIZATION; IMMUNOGENICITY; INFLUENZA VIRUS A; MOLECULAR WEIGHT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; WESTERN BLOTTING; ANIMAL; BAGG ALBINO MOUSE; BIOREACTOR; BLOOD; ENZYMOLOGY; GENETICS; IMMUNOLOGY; INFLUENZA VIRUS A H1N1; METABOLISM; PICHIA;

ESCHERICHIA COLI; INFLUENZA A VIRUS; MUS; ORTHOMYXOVIRIDAE; PICHIA PASTORIS;

ANIMALS; ANTIBODIES, VIRAL; BIOREACTORS; BLOTTING, WESTERN; DNA PRIMERS; ESCHERICHIA COLI; GLYCOSYLATION; INFLUENZA A VIRUS, H1N1 SUBTYPE; INFLUENZA VACCINES; MANNOSYLTRANSFERASES; MEMBRANE GLYCOPROTEINS; MICE; MICE, INBRED BALB C; NEURAMINIDASE; PICHIA; RECOMBINANT PROTEINS;

EID: 84860343045     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-011-0809-z     Document Type: Article

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