Enhanced production of Aspergillus niger laccase-like multicopper oxidases through mRNA optimization of the glucoamylase expression system

Biotechnology and Bioengineering

Volumn 110, Issue 2, 2013, Pages 543-551

  Tamayo Ramos, Juan Antonio ^a   Barends, Sharief ^b   de Lange, Dennis ^b,c   de Jel, Annemarie ^b   Verhaert, Raymond ^b   De Graaff, Leo ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b ProteoNic BV   (Netherlands)

^c Genencor International BV   (Netherlands)

Author keywords

Aspergillus niger; High throughput screening; MRNA 5 UTR; Multicopper oxidase

Indexed keywords

ASPERGILLUS NIGER; CODING GENES; EXPRESSION SYSTEM; FILAMENTOUS FUNGI; GENE PROMOTER; GLUCOAMYLASE; HIGH-THROUGHPUT METHOD; HIGH-THROUGHPUT SCREENING; LACCASE ACTIVITY; LACCASES; LIQUID CULTURE; MULTICOPPER OXIDASE; NIGER; PRODUCTION LEVEL; PROTEIN YIELD; SUPERNATANTS; TARGET GENES; TRANSCRIPT LEVEL; TRANSFORMANTS; TRANSLATION PROCESS;

GENES; RECOMBINANT PROTEINS;

ASPERGILLUS;

GLUCAN 1,4 ALPHA GLUCOSIDASE; LACCASE; MULTICOPPER OXIDASE A; MULTICOPPER OXIDASE B; MULTICOPPER OXIDASE C; MULTICOPPER OXIDASE D; MULTICOPPER OXIDASE J; OXIDOREDUCTASE; UNCLASSIFIED DRUG;

5' UNTRANSLATED REGION; ARTICLE; ASPERGILLUS NIGER; FUNGUS ISOLATION; HIGH THROUGHPUT SCREENING; NONHUMAN; PROTEIN EXPRESSION;

5' UNTRANSLATED REGIONS; ASPERGILLUS NIGER; CLONING, MOLECULAR; ESCHERICHIA COLI; FUNGAL PROTEINS; GLUCAN 1,4-ALPHA-GLUCOSIDASE; HIGH-THROUGHPUT SCREENING ASSAYS; OXIDOREDUCTASES; PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER;

ASPERGILLUS NIGER; FUNGI;

EID: 84871722011     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24723     Document Type: Article

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