5′ untranslated region of the Hsp12 gene contributes to efficient translation in Aspergillus oryzae

Applied Microbiology and Biotechnology

Volumn 70, Issue 3, 2006, Pages 333-336

  Koda, Akio ^a   Bogaki, Takayuki ^a   Minetoki, Toshitaka ^a   Hirotsune, Masato ^a  

^a Ozeki Corporation   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGY; MICROORGANISMS; PROTEINS; RNA; THERMAL EFFECTS;

ASPERGILLUS ORYZAE; EXOGENEOUS GENES; TRANSLATION EFFICIENCY;

GENES;

HEAT SHOCK PROTEIN 12 PROTEIN; PROTEIN; UNCLASSIFIED DRUG; BETA GLUCURONIDASE; HEAT SHOCK PROTEIN; HYBRID PROTEIN;

GENE;

5' UNTRANSLATED REGION; ANALYTIC METHOD; ARTICLE; ASPERGILLUS ORYZAE; COMPARATIVE STUDY; CONTROLLED STUDY; GENE; HEAT SHOCK PROTEIN 12 GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; RNA TRANSLATION; TEMPERATURE; CHEMISTRY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN SYNTHESIS;

ASPERGILLUS ORYZAE;

5' UNTRANSLATED REGIONS; ASPERGILLUS ORYZAE; BASE SEQUENCE; GENE EXPRESSION REGULATION, FUNGAL; GLUCURONIDASE; HEAT-SHOCK PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS;

EID: 33644778960     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0083-x     Document Type: Article

References (15)

1. Dansako T, Kato K, Satoh J, Sekine M, Yoshida K, Shinmyo A (2003) 5′ Untranslated region of the HSP18.2 gene contributes to efficient translation in plant cells. J Biosci Bioeng 95:52-58
2. Ishida H, Hata Y, Kawato A, Abe Y, Kashiwagi Y (2004) Isolation of a novel promoter for efficient protein production in Aspergillus oryzae. Biosci Biotechnol Biochem 68:1849-1857
3. Jefferson RA, Burgess SM, Hirsh D (1986) β-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci U S A 83:8447-8451
4. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901-3907
5. Johansson J, Mandin P, Renzoni A, Chiaruttini C, Springer M, Cossart P (2002) An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes. Cell 110:551-561
6. Koda A, Minetoki T, Ozeki K, Hirotsune M (2004) Translation efficiency mediated by the 5′ untranslated region greatly affects protein production in Aspergillus oryzae. Appl Microbiol Biotechnol 66:291-296
7. Machida M (2002) Progress of Aspergillus oryzae genomics. Adv Appl Microbiol 51:81-106
8. Machida M, Chang YC, Manabe M, Yasukawa M, Kunihiro S, Jigami Y (1996) Molecular cloning of a cDNA encoding enolase from the filamentous fungus, Aspergillus oryzae. Curr Genet 30:423-431
9. Minetoki T (2000) Development of the heterologous gene expression system for Aspergillus species (in Japanese). Kagaku to Seibutsu 38:831-838
10. Minetoki T, Kumagai C, Gomi K, Kitamoto K, Takahashi K (1998) Improvement of promoter activity by the introduction of multiple copies of the conserved region III sequence, involved in the efficient expression of Aspergillus oryzae amylase-encoding genes. Appl Microbiol Biotechnol 50:459-467
11. 32: evidence for a built-in RNA thermosensor. Genes Dev 13:655-665
12. Ozeki K, Kanda A, Hamachi M, Nunokawa Y (1996) Construction of a promoter probe vector autonomously maintained in Aspergillus and characterization of promoter regions derived from A. niger and A. oryzae genomes. Biosci Biotechnol Biochem 60:383-389
13. Tada S, Gomi K, Kitamoto K, Takahashi K, Tamura G, Kara S (1991) Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus oryzae. Mol Gen Genet 229:301-306
14. Tsuboi H, Koda A, Toda T, Minetoki T, Hirotsune M, Machida M (2005) Improvement of the Aspergillus oryzae enolase promoter (P-enoA) by the introduction of cis-element repeats. Biosci Biotechnol Biochem 69:206-208
15. Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406-3415