Structure-based function discovery of an enzyme for the hydrolysis of phosphorylated sugar lactones

Biochemistry

Volumn 51, Issue 8, 2012, Pages 1762-1773

  Xiang, Dao Feng ^a   Kolb, Peter ^c   Fedorov, Alexander A ^b   Xu, Chengfu ^a   Fedorov, Elena V ^b   Narindoshivili, Tamari ^a   Williams, Howard J ^a   Shoichet, Brian K ^c   Almo, Steven C ^b   Raushel, Frank M ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ACTIVE SITE STRUCTURE; AMIDOHYDROLASE SUPERFAMILY; BACILLUS HALODURANS; BINUCLEAR METAL CENTER; CATALYTIC FUNCTIONS; COMPUTATIONAL DOCKINGS; FUNCTION DISCOVERY; HIGH ENERGY; INTEGRATED STRATEGY; LIBRARY SCREENING; LISTERIA MONOCYTOGENES; STRUCTURE-BASED; SUGAR LACTONES; THREE-DIMENSIONAL CRYSTALS; THREE-DIMENSIONAL STRUCTURE;

BACTERIOLOGY; BIOINFORMATICS; CATALYSIS; CLONING; COBALT COMPOUNDS; DOCKING; ENZYMES; HYDROGEN BONDS; HYDROLYSIS; PHOSPHORYLATION; SUBSTRATES; THREE DIMENSIONAL;

SUGARS;

AMIDASE; AMIDASE BH0225; AMIDASE LMOF2365 2620; DEXTRO ARABINARIC ACID 1,4 LACTONE; DEXTRO ARABINARIC ACID 2,5 LACTONE; DEXTRO LYXONO 1,4 LACTONE 5 PHOSPHATE; GLUCONOLACTONASE; LACTONE; LEVO ARABINARIC ACID 1,4 LACTONE; LEVO ARABINARIC ACID 2,5 LACTONE; LEVO RIBONO 1,4 LACTONE 5 PHOSPHATE; PHOSPHATE; RIBARIC ACID 1,4 LACTONE; RIBARIC ACID 2,5 LACTONE; UNCLASSIFIED DRUG; XYLARIC 2,5 LACTONE; XYLARIC ACID 1,4 LACTONE; ZINC;

ARTICLE; BACILLUS; BACILLUS HALODURANS; BIOINFORMATICS; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HYDROGEN BOND; HYDROLYSIS; LISTERIA MONOCYTOGENES; MOLECULAR CLONING; MOLECULAR DOCKING; NONHUMAN; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; STRUCTURE ACTIVITY RELATION; SYNTHESIS;

AMIDOHYDROLASES; BACILLUS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; HYDROGEN BONDING; HYDROLYSIS; KINETICS; LACTONES; LISTERIA MONOCYTOGENES; MODELS, MOLECULAR; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; SUGAR PHOSPHATES;

BACILLUS HALODURANS C-125; LISTERIA MONOCYTOGENES;

EID: 84857608331     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi201838b     Document Type: Article

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