Dielectric analysis and multi-cell electrofusion of the yeast pichia pastoris for electrophysiological studies

Journal of Membrane Biology

Volumn 245, Issue 12, 2012, Pages 815-826

  Terpitz, Ulrich ^a,b   Letschert, Sebastian ^b   Bonda, Ulrich ^b   Spahn, Christoph ^b   Guan, Chonglin ^b   Sauer, Markus ^b   Zimmermann, Ulrich ^c   Bamberg, Ernst ^a,d   Zimmermann, Dirk ^d   Sukhorukov, Vladimir L ^b  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c ZIM Plant Technology   (Germany)

^d GOETHE UNIVERSITY   (Germany)

Author keywords

Cell wall digestion; Channelrhodopsin 2; Dielectrophoresis; Electrorotation; Giant protoplast; Patch clamp

Indexed keywords

RHODOPSIN;

ARTICLE; CELL WALL; CONTROLLED STUDY; DIELECTRIC CONSTANT; ELECTRIC CURRENT; ELECTROFUSION (CELL); ELECTROPHORESIS; ELECTROPHYSIOLOGY; FUNGAL STRAIN; HETEROLOGOUS EXPRESSION; HUMAN; HUMAN CELL; LIGHT; NONHUMAN; PATCH CLAMP; PICHIA PASTORIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTOPLAST; PROTOPLAST ISOLATION; STRAIN DIFFERENCE; YEAST CELL;

CELL FUSION; CELL SIZE; CELL WALL; ELECTRIC CONDUCTIVITY; ELECTROCHEMICAL TECHNIQUES; FUNGAL PROTEINS; HYDROLYSIS; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; PATCH-CLAMP TECHNIQUES; PICHIA; PROTOPLASTS; RHODOPSIN;

EUKARYOTA; PICHIA PASTORIS;

EID: 84871299322     PISSN: 00222631     EISSN: 14321424     Source Type: Journal    
DOI: 10.1007/s00232-012-9484-9     Document Type: Article

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