Mechanical disruption of Pichia pastoris yeast to recover the recombinant glycoprotein Bm86

Enzyme and Microbial Technology

Volumn 23, Issue 1-2, 1998, Pages 58-63

  Canales, Mario ^a   Buxadó, José A ^a   Heynngnezz, Lázaro ^a   Enríquez, Antonio ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Bead mill; Cell disintegration; Pichia pastoris; rBm86 protein

Indexed keywords

CELL MEMBRANES; DISINTEGRATION; FERMENTATION; METHANOL; YEAST;

BEAD MILLS; CELL DISINTEGRATION; WET MILLING;

PROTEINS;

GLYCOPROTEIN; METHANOL; RECOMBINANT PROTEIN;

ARTICLE; CELL MEMBRANE; ENDOMYCETALES; MECHANICAL STRESS; NONHUMAN; TEMPERATURE; YEAST;

PICHIA PASTORIS;

EID: 0032125853     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(98)00012-X     Document Type: Article

References (26)

1. 1. Schütte, H. and Kula, M. R. Pilot and process scale techniques for cell disruption. Biotechnol. Appl. Biochem. 1990, 12, 599-620
2. 2. White, D. M. and Marcus, D. Disintegration of microorganisms. In: Downstream Processes: Equipment and Techniques (Mizrahi, A., Ed.). Alan R. Liss, New York, 1988, 51-96
3. 3. Schütte, H. and Kula, M. R. Cell disruption and isolation of non-secreted products. In: Bioprocessing (Stephanopoulos, G., Ed.). VCH Verlagsgesellschaft mbH Weinheim (FRG) and VCH Publishers, New York, 1993, 506-526
4. 4. Wheelwright, S. M. Protein Purification: Design and Scale up of Downstream Processing. Carl Hanser Verlag, Munich, 1991, 60-72
5. 5. Engler, C. R. Cell breakage. In: Protein Purification Process Engineering (Harrison, R. G., Ed.). Marcel Dekker, New York, 1994, 37-55
6. 6. Marffy, F. and Kula, M. R. Enzymes yields from cells of Brewer's yeast disrupted by treatment in a horizontal disintegrator. Biotechnol. Bioeng. 1974, 16, 623-634
7. 7. Mogren, H., Lindblom, M., and Hedenskog, G. Mechanical disintegration of microorganisms in an industrial homogenizer. Biotechnol. Bioeng. 1974, 16, 261-274
8. 8. Limon-Lason, J., Hoare, M., Orsborn, C. B., Doyle, D. J., and Dunnill, P. Reactor properties of a high-speed bead mill for microbial cell rupture. Biotechnol. Bioeng. 1979, 21, 745-774
9. 9. Rehacek, J. and Schaffer, J. Disintegration of microorganisms in an industrial horizontal mill of novel design. Biotechnol. Bioeng. 1977, 19, 1523-1534
10. 10. Currie, J. A., Dunnill, P., and Lilly, D. Release of protein from Bakers' Yeast (S. cerevisiae) by disruption in an industrial agitator mill. Biotechnol. Bioeng. 1972, 14, 725-736
11. 11. van Gaver, D. and Huyghebaert, A. Optimization of yeast cell disruption with a newly designed bead mill. Enzyme Microb. Technol. 1990, 13, 665-670
12. 12. Rehacek, J., Beran, K., and Bicik, V. Disintegration of microorganisms and preparation of yeast cell walls in a new type of disintegrator. Appl. Microbiol. 1969, 17, 462-466
13. 13. Paez, R., Agraz, A., and Herrera, L. The recovery of the hepatitis B virus surface antigen (HBsAg) from a recombinant P. pastoris strain. Disruption and precipitation studies. Acta Biotechnol. 1993, 13, 117-122
14. 14. Kula, M. R. and Shütte, H. Purification of proteins and the disruption of microbial cells. Biotechnol. Prog. 1987, 3, 31-42
15. 15. Shütte, H., Kroner, K. H., Hustedt, H., and Kula, M. R. Experiences with a 20 litre industrial bead mill for the disruption of microorganisms. Enzyme Microb. Technol. 1983, 5, 143-148
16. 16. Weit, H. and Schwedes, J. Scale-up of power consumption in agitated ball mills. Chem. Eng. Technol. 1987, 10, 398-404
17. 17. Melendres, V., Unno, H., Shiragami, N., and Honda, H. A concept of critical velocity for cell disruption by bead mill. J. Chem. Eng. Jpn. 1992, 25, 354-356
18. 18. Mólls, H. and Hörnle, R. Wirkungsmechanismus der Nasszerkleinerung in der Rührwerkskugelmühle. Dechema Monographie 1972, 69, 631-661
19. 19. Canales, M., Enríquez, A., Ramos, E., Cabrera, D., Dandie, H., Soto, A., Falcón, V., Rodríguez, M., and de la Fuente, J. Large-scale production in Pichia pastoris of the recombinant vaccine Gavac™ against cattle tick. Vaccine 1997, 15, 414-432
20. 20. Lebuza, T. P., Santos, D. B., and Roop, R. N. Engineering factors in single-cell protein production. I. Fluid properties and concentration of yeast by evaporation. Biotechnol. Bioeng. 1970, 12, 123-134
21. 21. Perry, R. H., Chilton, C. H., and Kirkpatrick, S. D. Chemical Engineers' Handbook. 5th Ed., McGraw-Hill Book Company, New York, 1984, 3-88; 3-127.
22. 22. Bradford, M. M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254
23. 23. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685
24. 24. Towbin, H., Staehelin, T., and Gordon, J. Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications. Proc. Natl. Acad. Sci. 1979, 76, 4350-4354
25. 25. Spurr, A. R. A low viscosity epoxi resin embedding medium for electron microscopy. J. Ultrastructure Res. 1969, 26, 31-43
26. 26. Enríquez, A., Canales, M., Ramos, E., Dandie, H., Boué, O., Soto, A., and Cabrera, D. Production of a recombinant vaccine against Boophilus microplus. In: Recombinant Vaccines for the Control of Cattle Tick (de la Fuente, J., Ed.). Elfos Scientiae, La Habana, 1995, 79-95