Cell-cell electrofusion: Optimization of electric field amplitude and hypotonic treatment for mouse melanoma (B16-F1) and Chinese Hamster Ovary (CHO) cells

Journal of Membrane Biology

Volumn 236, Issue 1, 2010, Pages 107-116

  Ušaj, Marko ^a   Trontelj, Katja ^a   Miklavčič, Damijan ^a   Kandušer, Maša ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

B16 F1 cell; Cell survival; CHO cell; Electrofusion; Electroporation; Fluorescence microscopy; Hypotonic buffer; Spectrofluorometer

Indexed keywords

ANIMAL CELL; ARTICLE; CELL FUSION; CELL SURVIVAL; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; ELECTRIC FIELD; ELECTROPORATION; FLUORESCENCE MICROSCOPY; GENE TRANSFER; HUMAN; HUMAN CELL; MELANOMA CELL; MOUSE; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; SPECTROFLUOROMETRY;

ANIMALS; CELL FUSION; CELL LINE, TUMOR; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; ELECTROCHEMICAL TECHNIQUES; MICE; MICROSCOPY, FLUORESCENCE;

CRICETULUS GRISEUS;

EID: 77955308510     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00232-010-9272-3     Document Type: Article

References (80)

1. 3+ on surface charges, dielectrophoresis, and electrofusion of barley protoplasts Plant Physiol 87 389 394 10.1104/pp.87.2.389 1:CAS:528:DyaL1cXkvV2ru7c%3D 16666152
2. IG Abidor AI Barbul DV Zhelev P Doinov IN Bandrina EM Osipova SI Sukharev 1993 Electrical properties of cell pellets and cell electrofusion in a centrifuge Biochim Biophys Acta 1152 207 218 10.1016/0005-2736(93)90251-T 1:CAS:528:DyaK2cXjvV2h 8218321
3. QF Ahkong JA Lucy 1986 Osmotic forces in artificially induced cell fusion Biochim Biophys Acta 858 206 216 10.1016/0005-2736(86)90308-1 1:CAS:528:DyaL28XksVSjtLs%3D 3707962
4. C Barrau J Teissie B Gabriel 2004 Osmotically induced membrane tension facilitates the triggering of living cell electropermeabilization Bioelectrochemistry 63 327 332 10.1016/j.bioelechem.2003.11.009 1:CAS:528:DC%2BD2cXjsVWit7g%3D 15110297
5. C Blangero MP Rols J Teissié 1989 Cytoskeletal reorganization during electric-field-induced fusion of Chinese hamster ovary cells grown in monolayers Biochim Biophys Acta 981 295 302 10.1016/0005-2736(89)90040-0 1:STN:280:DyaL1M3nsValtA%3D%3D 2567186
6. J Castro-Garza HB Barrios-Garcia DE Cruz-Vega S Said-Fernandez P Carranza-Rosales CA Molina-Torres L Vera-Cabrera 2007 Use of a colorimetric assay to measure differences in cytotoxicity of Mycobacterium tuberculosis strains J Med Microbiol 56 733 737 10.1099/jmm.0.46915-0 1:CAS:528: DC%2BD2sXnsVKqu7s%3D 17510256
7. U Cegovnik S Novakovic 2004 Setting optimal parameters for in vitro electrotransfection of B16F1, SA1, LPB, SCK, L929 and CHO cells using predefined exponentially decaying electric pulses Bioelectrochemistry 62 73 82 10.1016/j.bioelechem.2003.10.009 1:CAS:528:DC%2BD2cXhsFWgs7c%3D 14990328
8. M Cemazar T Jarm D Miklavcic A Macek-Lebar A Ihan NA Kopitar G Sersa 1998 Effect of electric-field intensity on electropermeabilization and electrosensitivity of various tumor-cell lines in vitro Electromagn Biol Med 17 263 272 10.3109/15368379809022571
9. EH Chen E Grote W Mohler A Vignery 2007 Cell-cell fusion FEBS Lett 581 2181 2193 10.1016/j.febslet.2007.03.033 1:CAS:528:DC%2BD2sXlt1ChsL8%3D 17395182
10. J-M Escoffre T Portet L Wasungu J Teissie D Dean M-P Rols 2009 What is (still not) known of the mechanism by which electroporation mediates gene transfer and expression in cells and tissues Mol Biotechnol 41 286 295 10.1007/s12033-008-9121-0 1:CAS:528:DC%2BD1MXotVylsw%3D%3D 19016008
11. EA Evans VA Parsegian 1986 Thermal-mechanical fluctuations enhance repulsion between bimolecular layers Proc Natl Acad Sci USA 83 7132 7136 10.1073/pnas.83.19.7132 1:CAS:528:DyaL28Xlsl2jurc%3D 3463955
12. C Finaz A Lefevre J Teissie 1984 Electrofusion. A new, highly efficient technique for generating somatic cell hybrids Exp Cell Res 150 477 482 10.1016/0014-4827(84)90592-5 1:CAS:528:DyaL2cXpvFelsw%3D%3D 6692859
13. S Foung S Perkins K Kafadar P Gessner U Zimmermann 1990 Development of microfusion techniques to generate human hybridomas J Immunol Methods 134 35 42 10.1016/0022-1759(90)90109-9 1:STN:280:DyaK3M%2FjslSgsA%3D%3D 2172386
14. M Gabrijel U Repnik M Kreft S Grilc M Jeras R Zorec 2004 Quantification of cell hybridoma yields with confocal microscopy and flow cytometry Biochem Biophys Res Commun 314 717 723 10.1016/j.bbrc.2003.12.154 1:CAS:528: DC%2BD2cXltF2jsQ%3D%3D 14741694
15. M Gabrijel M Kreft R Zorec 2008 Monitoring lysosomal fusion in electrofused hybridoma cells Biochim Biophys Acta 1778 483 490 10.1016/j.bbamem.2007.10.013 1:CAS:528:DC%2BD1cXpt1Omtg%3D%3D 17996722
16. M Gabrijel M Bergant M Kreft M Jeras R Zorec 2009 Fused late endocytic compartments and immunostimulatory capacity of dendritic-tumor cell hybridomas J Membr Biol 229 11 18 10.1007/s00232-009-9171-7 1:CAS:528:DC%2BD1MXmsVCrtbY%3D 19418087
17. RJ Gillies N Didier M Denton 1986 Determination of cell number in monolayer cultures Anal Biochem 159 109 113 10.1016/0003-2697(86)90314-3 1:CAS:528:DyaL2sXmtlyitg%3D%3D 3812988
18. M Golzio M-P Mora C Raynaud C Delteil J Teissié M-P Rols 1998 Control by osmotic pressure of voltage-induced permeabilization and gene transfer in mammalian cells Biophys J 74 3015 3022 10.1016/S0006-3495(98)78009-9 1:CAS:528:DyaK1cXjs12lu78%3D 9635756
19. J Gong D Avigan D Chen Z Wu S Koido M Kashiwaba D Kufe 2000 Activation of antitumor cytotoxic T lymphocytes by fusions of human dendritic cells and breast carcinoma cells Proc Natl Acad Sci USA 97 2715 2718 10.1073/pnas. 050587197 1:CAS:528:DC%2BD3cXitVahs7o%3D 10688917
20. E Gottfried R Krieg C Eichelberg R Andreesen A Mackensen SW Krause 2002 Characterization of cells prepared by dendritic cell-tumor cell fusion Cancer Immun 2 15 12747760
21. T Hayashi H Tanaka J Tanaka R Wang BJ Averbook PA Cohen S Shu 2002 Immunogenicity and therapeutic efficacy of dendritic-tumor hybrid cells generated by electrofusion Clin Immunol 104 14 20 10.1006/clim.2002.5224 1:CAS:528:DC%2BD38Xmsl2qu7w%3D 12139943
22. K James GT Bell 1987 Human monoclonal antibody production J Immunol Methods 100 5 40 10.1016/0022-1759(87)90170-0 1:CAS:528:DyaL2sXkvFCku7c%3D 3298441
23. Jantscheff P, Spagnoli G, Zajac P, Rochlitz C (2002) Cell fusion: an approach to generating constitutively proliferating human tumor antigen-presenting cells. Cancer Immunol Immunother 51:367-375
24. MJ Jaroszeski R Gilbert PG Fallon R Heller 1994 Mechanically facilitated cell-cell electrofusion Biophys J 67 1574 1581 10.1016/S0006-3495(94)80630-7 1:STN:280:DyaK2M7hvFejug%3D%3D 7819489
25. Jaroszeski MJ, Gilbert R, Heller R (1995) Cytometric detection and quantitation of cell-cell electrofusion products. In: Nickoloff JA (ed) Animal cell electroporation and electrofusion protocols. Humana Press, Totowa, NJ, pp 355-363
26. M Kanduser M Sentjurc D Miklavcic 2006 Cell membrane fluidity related to electroporation and resealing Eur Biophys J 35 196 204 10.1007/s00249-005-0021-y 16215750
27. S Knutton D Jackson JM Graham KJ Micklem CA Pasternak 1976 Microvilli and cell swelling Nature 262 52 54 10.1038/262052a0 1:STN:280:DyaE283itVCrsA%3D%3D 180426
28. T Kotnik F Bobanovic D Miklavcic 1997 Sensitivity of transmembrane voltage induced by applied electric fields-a theoretical analysis Bioelectrochem Bioenerg 43 6 10.1016/S0302-4598(97)00023-8
29. IH Lambert 2007 Activation and inactivation of the volume-sensitive taurine leak pathway in NIH3T3 fibroblasts and Ehrlich Lettre ascites cells Am J Physiol Cell Physiol 293 C390 C400 10.1152/ajpcell.00104.2007 1:CAS:528:DC%2BD2sXosV2msr4%3D 17537804
30. WT Lee K Shimizu H Kuriyama H Tanaka J Kjaergaard S Shu 2005 Tumor-dendritic cell fusion as a basis for cancer immunotherapy Otolaryngol Head Neck Surg 132 755 764 10.1016/j.otohns.2005.01.018 15886631
31. A Macek-Lebar D Miklavcic 2001 Cell electropermeabilization to small molecules in vitro: control by pulse parameters Radiol Oncol 35 10
32. Mally MI, McKnight ME, Glassy MC (1992) Protocols of electroporation and electrofusion for producing human hybridomas. In: Chang D, Chassy B, Saunders J, Sowers A (eds) Guide to electroporation and electrofusion. Academic Press, San Diego, pp 507-522
33. S Martens HT McMahon 2008 Mechanisms of membrane fusion: disparate players and common principles Nat Rev Mol Cell Biol 9 543 556 10.1038/nrm2417 1:CAS:528:DC%2BD1cXnsFGgur0%3D 18496517
34. M Marty G Sersa JR Garbay J Gehl CG Collins M Snoj V Billard PF Geertsen JO Larkin D Miklavcic I Pavlovic SM Paulin-Kosir M Cemazar N Morsli Z Rudolf C Robert GC O'Sullivan LM Mir 2006 Electrochemotherapy-an easy, highly effective and safe treatment of cutaneous and subcutaneous metastases: results of ESOPE (European Standard Operating Procedures of Electrochemotherapy) study EJC Suppl 4 3 13 1:CAS:528:DC%2BD2sXktlCrsA%3D%3D
35. EA Matibiri SH Mantell 1995 Comparative effects of fusion facilitators on electrofusion attributes of N. tabacum mesophyll protoplasts Plant Cell Tissue Organ Culture 40 125 131 10.1007/BF00037665 1:CAS:528:DyaK2MXkvVGntL0%3D
36. TJ McIntosh S Advani RE Burton DV Zhelev D Needham SA Simon 1995 Experimental tests for protrusion and undulation pressures in phospholipid bilayers Biochemistry 34 8520 8532 10.1021/bi00027a002 1:CAS:528: DyaK2MXmsVeiu7g%3D 7612594
37. TJ McIntosh KG Kulkarni SA Simon 1999 Membrane fusion promoters and inhibitors have contrasting effects on lipid bilayer structure and undulations Biophys J 76 2090 2098 10.1016/S0006-3495(99)77365-0 1:CAS:528: DyaK1MXit1Ojs7g%3D 10096904
38. H Mekid LM Mir 2000 In vivo cell electrofusion Biochim Biophys Acta 1524 118 130 1:CAS:528:DC%2BD3cXoslGisbY%3D 11113558
39. D Miklavcic L Towhidi 2010 Numerical study of the electroporation pulse shape effect on molecular uptake of biological cells Radiol Oncol 44 8
40. LM Mir 2009 Nucleic acids electrotransfer-based gene therapy (electrogenetherapy): past, current, and future Mol Biotechnol 43 167 176 10.1007/s12033-009-9192-6 1:CAS:528:DC%2BD1MXhtFCitrrM 19562526
41. Y Mizukami H Kito M Okauchi 1993 Factors affecting the electrofusion efficiency of Porphyra protoplasts J Appl Psychol 5 29 36
42. GA Neil U Zimmermann 1993 Electrofusion Methods Enzymol 220 174 196 10.1016/0076-6879(93)20082-E 1:CAS:528:DyaK3sXmt1Sks7k%3D 8350753
43. Neumann E, Sowers AE, Jordan CA (1989) Electroporation and electrofusion in cell biology. Springer-Verlag, New York
44. O'Hare MJ, Ormerod MG, Imrie PR, Peacock JH, Asche W (1989) Electropermeabilization and electrosensitivity of different types of mammalian cells. In: Neumann E, Sowers AE, Jordan CA (eds) Electroporation and electrofusion in cell biology. Plenum Press, New York, pp 319-330
45. T Ohno-Shosaku Y Okada 1985 Electric pulse-induced fusion of mouse lymphoma cells: roles of divalent cations and membrane lipid domains J Membr Biol 85 269 280 10.1007/BF01871522 1:CAS:528:DyaL2MXltValu70%3D 3897548
46. G Pucihar D Miklavcic T Kotnik 2009 A time-dependent numerical model of transmembrane voltage inducement and electroporation of irregularly shaped cells IEEE Trans Biomed Eng 56 1491 1501 10.1109/TBME.2009.2014244 19203876
47. C Ramos D Bonenfant J Teissie 2002 Cell hybridization by electrofusion on filters Anal Biochem 302 213 219 10.1006/abio.2001.5553 1:CAS:528: DC%2BD38Xhs1ClurY%3D 11878799
48. MP Rols J Teissie 1989 Ionic-strength modulation of electrically induced permeabilization and associated fusion of mammalian cells Eur J Biochem 179 109 115 10.1111/j.1432-1033.1989.tb14527.x 1:CAS:528:DyaL1MXntlWjug%3D%3D 2645133
49. MP Rols J Teissie 1990 Modulation of electrically induced permeabilization and fusion of Chinese hamster ovary cells by osmotic pressure Biochemistry 29 4561 4567 10.1021/bi00471a009 1:CAS:528:DyaK3cXitFCntb0%3D 2372540
50. B Rubinsky G Onik P Mikus 2007 Irreversible electroporation: a new ablation modality-clinical implications Technol Cancer Res Treat 6 37 48 17241099
51. S Salomskaite-Davalgiene K Cepurniene S Satkauskas MS Venslauskas LM Mir 2009 Extent of cell electrofusion in vitro and in vivo is cell line dependent Anticancer Res 29 3125 3130 19661325
52. A Salvi J Quillan W Sadée 2002 Monitoring intracellular pH changes in response to osmotic stress and membrane transport activity using 5-chloromethylfluorescein AAPS J 4 21 28
53. JJ Schmitt U Zimmermann 1989 Enhanced hybridoma production by electrofusion in strongly hypo-osmolar solutions Biochim Biophys Acta 983 42 50 10.1016/0005-2736(89)90378-7 1:CAS:528:DyaL1MXkvFGjt7o%3D 2758049
54. R Schnettlera U Zimmermann 1992 Zinc ions stimulate electrofusion of Hansenula polymorpha protoplasts FEMS Microbiol Lett 106 47 51 10.1111/j.1574-6968.1993.tb05933.x
55. TH Scott-Taylor R Pettengell I Clarke G Stuhler MC La Barthe P Walden AG Dalgleish 2000 Human tumour and dendritic cell hybrids generated by electrofusion: potential for cancer vaccines Biochim Biophys Acta 1500 265 279 1:CAS:528:DC%2BD3cXhsVahsLs%3D 10699368
56. AE Sowers 1986 A long-lived fusogenic state is induced in erythrocyte ghosts by electric pulses J Cell Biol 102 1358 1362 10.1083/jcb.102.4.1358 1:CAS:528:DyaL28XhvVajsbo%3D 3958050
57. AE Sowers 1989 Evidence that electrofusion yield is controlled by biologically relevant membrane factors Biochim Biophys Acta 985 334 338 10.1016/0005-2736(89)90422-7 1:CAS:528:DyaK3cXisleq 2804114
58. DA Stenger RT Kubiniec WJ Purucker H Liang SW Hui 1988 Optimization of electrofusion parameters for efficient production of murine hybridomas Hybridoma 7 505 518 10.1089/hyb.1988.7.505 1:STN:280:DyaL1M%2FmsFOntQ%3D%3D 3198135
59. DA Stenger KV Kaler SW Hui 1991 Dipole interactions in electrofusion. Contributions of membrane potential and effective dipole interaction pressures Biophys J 59 1074 1084 10.1016/S0006-3495(91)82322-0 1:STN:280: DyaK3MzivFGnsg%3D%3D 1868154
60. RH Stevens E Macy C Morrow A Saxon 1979 Characterization of a circulating subpopulation of spontaneous antitetanus toxoid antibody producing B cells following in vivo booster immunization J Immunol 122 2498 2504 1:STN:280:DyaE1M7psFersA%3D%3D 312871
61. G Stuhler P Walden 1994 Recruitment of helper T cells for induction of tumour rejection by cytolytic T lymphocytes Cancer Immunol Immunother 39 342 345 10.1007/BF01519989 1:STN:280:DyaK2M%2FnvFCmsQ%3D%3D 7987867
62. SI Sukharev IN Bandrina AI Barbul LI Fedorova IG Abidor AV Zelenin 1990 Electrofusion of fibroblasts on the porous membrane Biochim Biophys Acta 1034 125 131 1:CAS:528:DyaK3cXksFKmt70%3D 2354188
63. VL Sukhorukov WM Arnold U Zimmermann 1993 Hypotonically induced changes in the plasma membrane of cultured mammalian cells J Membr Biol 132 27 40 1:CAS:528:DyaK3sXhs1KjsLc%3D 8459447
64. VL Sukhorukov R Reuss D Zimmermann C Held KJ Müller M Kiesel P Gener A Steinbach WA Schenk E Bamberg U Zimmermann 2005 Surviving high-intensity field pulses: strategies for improving robustness and performance of electrotransfection and electrofusion J Membr Biol 206 187 201 10.1007/s00232-005-0791-2 1:CAS:528:DC%2BD28XhtFWmsbo%3D 16456714
65. VL Sukhorukov R Reuss JM Endter S Fehrmann A Katsen-Globa P Gessner A Steinbach KJ Muller A Karpas U Zimmermann H Zimmermann 2006 A biophysical approach to the optimisation of dendritic-tumour cell electrofusion Biochem Biophys Res Commun 346 829 839 10.1016/j.bbrc.2006.05.193 1:CAS:528: DC%2BD28XmtlWrsbg%3D 16780801
66. J Teissie C Blangero 1984 Direct experimental evidence of the vectorial character of the interaction between electric pulses and cells in cell electrofusion Biochim Biophys Acta 775 446 448 10.1016/0005-2736(84)90203-7 1:STN:280:DyaL2c3otFSjtA%3D%3D 6466684
67. J Teissie C Ramos 1998 Correlation between electric field pulse induced long-lived permeabilization and fusogenicity in cell membranes Biophys J 74 1889 1898 10.1016/S0006-3495(98)77898-1 1:CAS:528:DyaK1cXislSrsLw%3D 9545050
68. J Teissie MP Rols 1986 Fusion of mammalian cells in culture is obtained by creating the contact between cells after their electropermeabilization Biochem Biophys Res Commun 140 258 266 10.1016/0006-291X(86)91084-3 1:STN:280:DyaL2s%2FkvVOmtg%3D%3D 3778446
69. J Teissie VP Knutson TY Tsong MD Lane 1982 Electric pulse-induced fusion of 3T3 cells in monolayer culture Science 216 537 538 10.1126/science.7071601 1:STN:280:DyaL387ns1agsw%3D%3D 7071601
70. K Trontelj M Rebersek M Kanduser VC Serbec M Sprohar D Miklavcic 2008 Optimization of bulk cell electrofusion in vitro for production of human-mouse heterohybridoma cells Bioelectrochemistry 74 124 129 10.1016/j.bioelechem.2008. 06.003 1:CAS:528:DC%2BD1cXhtlaqsL%2FP 18667367
71. N Urano R Higashikawa H Hirai 1998 Effects of mitochondria on electrofusion of yeast protoplasts Enzyme Microbial Technol 23 107 112 10.1016/S0141-0229(98)00021-0 1:CAS:528:DyaK1cXks1Kls7o%3D
72. M Usaj K Trontelj R Hudej M Kanducer D Miklavcic 2009 Cell size dynamics and viability of cells exposed to hypotonic treatment and electroporation for electrofusion optimization Radiol Oncol 43 108 119 10.2478/v10019-009-0017-9
73. J Vienken U Zimmermann 1985 An improved electrofusion technique for production of mouse hybridoma cells FEBS Lett 182 278 280 10.1016/0014-5793(85) 80315-X 1:CAS:528:DyaL2MXitVahsrY%3D 3979550
74. J Wang C Lu 2006 Microfluidic cell fusion under continuous direct current voltage Appl Physics Lett 89 234102 234103 10.1063/1.2402122
75. Y Wu JG Montes RA Sjodin 1992 Determination of electric field threshold for electrofusion of erythrocyte ghosts. Comparison of pulse-first and contact-first protocols Biophys J 61 810 815 10.1016/S0006-3495(92)81885-4 1:STN:280:DyaK38zmvFehug%3D%3D 1504251
76. X Yu PA McGraw FS House JE Crowe Jr 2008 An optimized electrofusion-based protocol for generating virus-specific human monoclonal antibodies J Immunol Methods 336 142 151 10.1016/j.jim.2008.04.008 1:CAS:528:DC%2BD1cXotlKqtrg%3D 18514220
77. Q Zheng DC Chang 1991 Reorganization of cytoplasmic structures during cell-cell fusion J Cell Sci 100 431 442 1808197
78. U Zimmermann 1982 Electric field-mediated fusion and related electrical phenomena Biochim Biophys Acta 694 227 277 1:CAS:528:DyaL3sXhtlSisQ%3D%3D 6758848
79. Zimmermann U, Neil GA (1996) Electromanipulation of cells. CRC, Boca Raton, FL
80. U Zimmermann U Friedrich H Mussauer P Gessner K Hamel V Sukhoruhov 2000 Electromanipulation of mammalian cells: fundamentals and application IEEE Trans Plasma Sci 28 72 82 10.1109/27.842868 1:CAS:528:DC%2BD3cXktVOms78%3D