Electrofused giant protoplasts of Saccharomyces cerevisiae as a novel system for electrophysiological studies on membrane proteins

Biochimica et Biophysica Acta - Biomembranes

Volumn 1778, Issue 6, 2008, Pages 1493-1500

  Terpitz, Ulrich ^a   Raimunda, Daniel ^b   Westhoff, Markus ^c   Sukhorukov, Vladimir L ^c   Beaugé, Luis ^b   Bamberg, Ernst ^a,d   Zimmermann, Dirk ^a,d  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b INSTITUTO DE INVESTIGACIÓN MÉDICA MERCEDES Y MARTÍN FERREYRA   (Argentina)

^c UNIVERSITY OF WÜRZBURG   (Germany)

^d GOETHE UNIVERSITY   (Germany)

Author keywords

Channelrhodopsin 2; Electrofusion; Giant protoplast; Patch clamp; Saccharomyces cerevisiae; Yeast

Indexed keywords

CHANNELRHODOPSIN 2; FLUORESCEIN; MEMBRANE PROTEIN; RHODOPSIN; YELLOW FLUORESCENT PROTEIN;

ARTICLE; BLUE LIGHT; CELL ADHESION; CELL MEMBRANE; CELL SIZE; CELL STRUCTURE; CONTROLLED STUDY; DIFFUSION; ELECTRIC FIELD; ELECTROFUSION (CELL); ELECTROPHYSIOLOGY; FLUORESCENCE; GENE EXPRESSION; MEMBRANE CURRENT; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; PROTOPLAST; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; STRUCTURE ANALYSIS;

CELL MEMBRANE; ELECTROPHYSIOLOGY; PATCH-CLAMP TECHNIQUES; PROTOPLASTS; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 43649091262     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbamem.2008.03.015     Document Type: Article

References (54)

1. Frommer W.B., and Ninnemann O. Heterologous expression of genes in bacterial, fungal, animal, and plant cells. Annu. Rev. Plant Physiol. Plant Mol. Biol. 46 (1995) 419-444
2. 2+-transporting ATPases. Am. J. Physiol. Cell Physiol. 287 (2004) C580-C589
3. Bertl A., Bihler H., Kettner C., and Slayman C.L. Electrophysiology in the eukaryotic model cell Saccharomyces cerevisiae. Eur. J. Physiol. 436 (1998) 999-1013
4. Peberdy J.F. Mycolytic enzymes. In: Peberdy J.F., and Ferenczy L. (Eds). Fungal Protoplasts - Applications in Biochemistry and Genetics. Mycology Series 6 (1985) 31-44
5. Yabe I., Horiuchi K., Nakahara K., Hiyama T., Yamanaka T., Wang P.C., Toda K., Hirata A., Ohsumi Y., Hirata R., Anraku Y., and Kusaka I. Patch clamp studies on V-type ATPase of vacuolar membrane of haploid Saccharomyces cerevisiae - preparation and utilization of a giant cell containing a giant vacuole. J. Biol. Chem. 274 (1999) 34903-34910
6. +-pump heterologously expressed in giant yeast vacuoles. J. Biochem. 134 (2003) 615-623
7. Bertl A., and Slayman C.L. Complex modulation of cation channels in the tonoplast and plasma membrane of Saccharomyces cerevisiae: single-channel studies. J. Exp. Biol. 172 (1992) 271-287
8. - co-transporter in yeast: characterization by patch clamp. Mycologia 93 (2001) 626-633
9. Pilwat G., Richter H.-P., and Zimmermann U. Giant culture cells by electric field-induced fusion. FEBS Lett. 133 (1981) 169-174
10. Urano N., Kamimura M., and Washizu M. Physical parameters affecting electrofusion of yeasts - ζ-potential on the surface of yeast protoplasts and osmotic-pressure of the solution. J. Biotechnol. 18 (1991) 213-223
11. Zimmermann D., Terpitz U., Zhou A., Reuss R., Müller K., Sukhorukov V.L., Gessner P., Nagel G., Zimmermann U., and Bamberg E. Biophysical characterisation of electrofused giant HEK293-cells as a novel electrophysiological expression system. Biochem. Biophys. Res. Com. 348 (2006) 673-681
12. Fuhr G., Müller T., Schnelle T., Hagedorn R., Voigt A., Fiedler S., Arnold W.M., Zimmermann U., Wagner B., and Heuberger A. Radiofrequency microtools for particle and live cell manipulation. Naturwissenschaften 81 (1994) 528-535
13. Li Y., and Kaler K.V.I.S. Dielectrophoretic fluidic cell fractionation system. Anal. Chim. Acta 507 (2004) 151-161
14. Sukhorukov V.L., Reuss R., Zimmermann D., Held C., Müller K.J., Kiesel M., Geßner P., Steinbach A., Schenk W.A., Bamberg E., and Zimmermann U. Surviving high-intensity field pulses: strategies for improving robustness and performance of electrotransfection and electrofusion. J. Membr. Biol. 206 (2005) 187-201
15. Trevor K.T., Cover C., Ruiz Y.W., Akporiaye E.T., Hersh E.M., Landais D., Taylor R.R., King A.D., and Walters R.E. Generation of dendritic cell-tumor cell hybrids by electrofusion for clinical vaccine application. Cancer Immunol. Immunother. 53 (2004) 705-714
16. Zimmermann U., and Neil G.A. Electrofusion of cells: state of the art and future directions. In: Zimmermann U., and Neil G.A. (Eds). Electromanipulation of Cells (1996), CRC Press Inc., New York 173-257
17. Jones T.B. Basic theory of dielectrophoresis and electrorotation. IEEE Eng. Med. Biol. Mag. 22 (2000) 33-42
18. Schnettler R., and Zimmermann U. Influence of the composition of the fusion medium on the yield of electrofused yeast hybrids. FEMS Microbiol. Lett. 27 (1985) 195-198
19. Weber H., Förster W., Berg H., and Jacob H.-E. Parasexual hybridization of yeasts by electric field stimulated fusion of protoplasts. Curr. Genet. 4 (1981) 165-166
20. Urano N., Ueno R., and Kimura S. Electrofusion prompts regeneration of yeast protoplasts: effect of electric pulse and media on the regeneration efficiency. Electrochemistry 68 (2000) 898-900
21. Song L., Ahkong Q.F., Georgescauld D., and Lucy J.A. Membrane fusion without cytoplasmic fusion (hemi-fusion) in erythrocytes that are subjected to electrical breakdown. Biochim. Biophys. Acta 1065 (1991) 54-62
22. Nagel G., Szellas T., Huhn W., Kateriya S., Adeishvili N., Berthold P., Ollig D., Hegemann P., and Bamberg E. Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 13940-13945
23. Lee K.M., and DaSilva N.A. Evaluation of the Saccharomyces cerevisiae ADH2 promoter for protein synthesis. Yeast 22 (2005) 431-440
24. Price V.L., Taylor W.E., Clevenger W., Worthington M., and Young E.T. (1990). Expression of heterologous proteins in Saccharomyces cerevisiae using ADH2 promoter. Meth. Enzymol. 185 (1990) 308-318
25. Peberdy J.F. Fungal protoplasts: isolation, reversion, and fusion. Annu. Rev. Microbiol. 33 (1979) 21-39
26. Popov B.K., and Konstantinova R.D. Preparation and regeneration of yeast protoplasts: a review. Zentralbl. Mikrobiol. 141 (1986) 217-224
27. Fahnestock S.R., and Bedzyk L.A. Production of synthetic spider dragline silk protein in Pichia pastoris. Appl. Microbiol. Biotechnol. 47 (1997) 33-39
28. Strugatsky D., Gottschalk K.E., Goldshleger R., Bibi E., and Karlish S.J. Expression of Na+,K+-ATPase in Pichia pastoris: analysis of wild type and D369N mutant proteins by Fe2+-catalyzed oxidative cleavage and molecular modeling. J. Biol. Chem. 278 (2003) 46064-46073
29. Andreasen A.A., and Stier T.J.B. Anaerobic nutrition of Saccharomyces cerevisiae. 1. Ergosterol requirement for growth in a defined medium. J. Cell. Comp. Physiol. 41 (1953) 23-36
30. Hossack J.A., and Rose A.H. Fragility of plasma membranes of Saccharomyces cerevisiae enriched with different sterols. J. Bacteriol. 127 (1976) 67-75
31. Rodriguez R.J., Low C., Bottema C.D.K., and Parks L.W. Multiple functions for sterols in Saccharomyces cerevisiae. Biochim. Biophys. Acta 837 (1985) 336-343
32. Yamaguchi H., Hiratani T., Baba M., and Osumi M. Effect of aculeacin A on reverting protoplasts of Candida albicans. Microbiol. Immunol. 31 (1987) 625-638
33. Svoboda A., Slaninová I., and Holubářová A. Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae. Acta Biol. Hung. 52 (2001) 325-333
34. Meikle A.J., Reed R.H., and Gadd G.M. Osmotic adjustment and the accumulation of organic solutes in whole cells and protoplasts of Saccharomyces cerevisiae. J. Gen. Microbiol. 134 (1988) 3049-3060
35. Tkacz J.S., Cybulska E.B., and Lampen J.O. Specific staining of wall mannan in yeast cells with fluorescein-conjugated concanavalin A. J. Bacteriol. 105 (1971) 1-5
36. Darken M.A. Absorption and transport of fluorescent brighteners by microorganisms. Appl. Microbiol. 10 (1962) 387-393
37. Harrington B.J., and Raper K.B. Use of a fluorescent brightener to demonstrate cellulose in the cellular slime molds. Appl. Microbiol. 16 (1968) 106-113
38. Schmitt J.J., and Zimmermann U. Enhanced hybridoma production by electrofusion in strongly hypo-osmolar solutions. Biochem. Biophys. Acta 983 (1989) 42-50
39. Klöck G., and Zimmermann U. Facilitated electrofusion of vacuolated & evacuolated oat mesophyll protoplasts in hypo-osmolar media after alignment with an alternating field of modulated strength. Biochem. Biophys. Acta 1025 (1990) 87-93
40. Soveral G., Veiga A., Loureiro-Dias M.C., Tanghe A., van Dijck P., and Moura T.F. Water channels are important for osmotic adjustments of yeast cells at low temperature. Mircobiol. 152 (2006) 1515-1521
41. Pohl P. Combined transport of water and ions through membrane channels. Biol. Chem. 385 (2004) 921-926
42. Ferenczy L. Protoplast fusion in yeasts. In: Peberdy J.F., and Ferenczy L. (Eds). Fungal Protoplasts - Applications in Biochemistry and Genetics. Mycology Series 6 (1985) 279-306
43. van Solingen P., and van der Plaat J.B. Fusion of yeast spheroplasts. J. Bacteriol. 130 (1977) 946-947
44. Weber H., and Berg H. Electrofusion of yeasts protoplasts. In: Nickoloff J.A. (Ed). Electroporation Protocols for Microorganisms. Meth. Mol. Biol. 47 (1995) 93-104
45. Garrill A., and Davies J.M. Patch clamping fungal membranes - a new perspective on ion-transport. Mycol. Res. 98 (1994) 257-263
46. Horisberger M., Rosset J., and Bauer H. Localization of mannan at the surface of yeast protoplasts by scanning electron-microscopy. Arch. Microbiol. 109 (1976) 9-14
47. Nečas O. Cell wall synthesis in yeast protoplasts. Bacteriol. Rev. 35 (1971) 149-170
48. Nečas O., Svoboda A., and Pivničková E. Plasma membrane particles in yeast protoplasts. Acta Histochem. S23 (1981) 151-155
49. Pringle A.T., Forsdyke J., and Rose A.H. Scanning electron microscope study of Saccharomyces cerevisiae spheroplast formation. J. Bacteriol. 140 (1979) 289-293
50. Streiblová E. Surface structure of yeast protoplasts. J. Bacteriol. 95 (1968) 700-707
51. Hölzel R. Electrorotation of single yeast cells at frequencies between 100 Hz and 1.6 GHz. Biophys. J. 73 (1997) 1103-1109
52. Rae J.L., and Levis R.A. Single-cell electroporation. Eur. J. Physiol. 443 (2002) 644-670
53. 31P NMR analysis of membrane phospholipid organisation in viable, reversibly electropermeabilized Chinese hamster ovary cells. Biochemistry 27 (1988) 1222-1228
54. Archer S., Tong-Tong L., Evans A.T., Britland S.T., and Morgan H. Cell reactions to dielectrophoretic manipulation. Biochem. Biophys. Res. Comm. 257 (1999) 687-698