Organotypic hippocampal slice culture from the adult mouse brain: A versatile tool for translational neuropsychopharmacology

Progress in Neuro-Psychopharmacology and Biological Psychiatry

Volumn 41, Issue , 2013, Pages 36-43

  Kim, Hyunjeong ^a   Kim, Eosu ^a   Park, Minsun ^a   Lee, Eun ^a   Namkoong, Kee ^a  

^a Yonsei University College of Medicine   (South Korea)

Author keywords

Adult brain; Hippocampus; Mice; Organotypic slice culture; Serum free

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ASTROCYTE; BRAIN SLICE; BRAIN TISSUE; CELL VIABILITY; CLINICAL OBSERVATION; CULTURE MEDIUM; EX VIVO STUDY; HIPPOCAMPUS; IN VIVO STUDY; MALE; METABOLIC SYNDROME X; MOUSE; NERVE CELL; NERVOUS SYSTEM DEVELOPMENT; NERVOUS TISSUE; NEUROSCIENCE; NONHUMAN; PRESYNAPTIC NERVE; PSYCHOPHARMACOLOGY;

AGE FACTORS; ANIMALS; BRAIN; CULTURE MEDIA, SERUM-FREE; HIPPOCAMPUS; MALE; MICE; MICE, INBRED ICR; ORGAN CULTURE TECHNIQUES; PSYCHOPHARMACOLOGY; TRANSLATIONAL MEDICAL RESEARCH;

EID: 84870975084     PISSN: 02785846     EISSN: 18784216     Source Type: Journal    
DOI: 10.1016/j.pnpbp.2012.11.004     Document Type: Article

References (39)

1. Chapleau C.A., Carlo M.E., Larimore J.L., Pozzo-Miller L. The actions of BDNF on dendritic spine density and morphology in organotypic slice cultures depend on the presence of serum in culture media. J Neurosci Methods 2008, 169:182-190.
2. Danzer S.C. Postnatal and adult neurogenesis in the development of human disease. Neuroscientist 2008, 14:446-458.
3. Das M., Bhargava N., Gregory C., Riedel L., Molnar P., Hickman J.J. Adult rat spinal cord culture on an organosilane surface in a novel serum-free medium. In Vitro Cell Dev Biol Anim 2005, 41:343-348.
4. del Rio J.A., Heimrich B., Soriano E., Schwegler H., Frotscher M. Proliferation and differentiation of glial fibrillary acidic protein-immunoreactive glial cells in organotypic slice cultures of rat hippocampus. Neuroscience 1991, 43:335-347.
5. Dudek F.E., Hellier J.L., Williams P.A., Ferraro D.J., Staley K.J. The course of cellular alterations associated with the development of spontaneous seizures after status epilepticus. Prog Brain Res 2002, 135:53-65.
6. Edwards D., Das M., Molnar P., Hickman J.J. Addition of glutamate to serum-free culture promotes recovery of electrical activity in adult hippocampal neurons in vitro. J Neurosci Methods 2010, 190:155-163.
7. Evans M.S., Collings M.A., Brewer G.J. Electrophysiology of embryonic, adult and aged rat hippocampal neurons in serum-free culture. J Neurosci Methods 1998, 79:37-46.
8. Fabian-Fine R., Volknandt W., Fine A., Stewart M.G. Age-dependent pre- and postsynaptic distribution of AMPA receptors at synapses in CA3 stratum radiatum of hippocampal slice cultures compared with intact brain. Eur J Neurosci 2000, 12:3687-3700.
9. Finley M., Fairman D., Liu D., Li P., Wood A., Cho S. Functional validation of adult hippocampal organotypic cultures as an in vitro model of brain injury. Brain Res 2004, 1001:125-132.
10. Fusco S., Ripoli C., Podda M.V., Ranieri S.C., Leone L., Toietta G., et al. A role for neuronal cAMP responsive-element binding (CREB)-1 in brain responses to calorie restriction. Proc Natl Acad Sci U S A 2012, 109:621-626.
11. Gahwiler B.H., Capogna M., Debanne D., McKinney R.A., Thompson S.M. Organotypic slice cultures: a technique has come of age. Trends Neurosci 1997, 20:471-477.
12. Gleeson J.G., Lin P.T., Flanagan L.A., Walsh C.A. Doublecortin is a microtubule-associated protein and is expressed widely by migrating neurons. Neuron 1999, 23:257-271.
13. Ide C.F., Scripter J.L., Coltman B.W., Dotson R.S., Snyder D.C., Jelaso A. Cellular and molecular correlates to plasticity during recovery from injury in the developing mammalian brain. Prog Brain Res 1996, 108:365-377.
14. Jessberger S., Gage F.H. Stem-cell-associated structural and functional plasticity in the aging hippocampus. Psychol Aging 2008, 23:684-691.
15. Kleinberger-Doron N., Schramm M. Culture of mature hippocampus slices for 4days in a newly developed medium: preservation of transmitter release and leucine incorporation into protein. Brain Res 1990, 533:239-247.
16. Knable M.B., Barci B.M., Webster M.J., Meador-Woodruff J., Torrey E.F. Molecular abnormalities of the hippocampus in severe psychiatric illness: postmortem findings from the Stanley Neuropathology Consortium. Mol Psychiatry 2004, 9(609-20):544.
17. Kovacs R., Papageorgiou I., Heinemann U. Slice cultures as a model to study neurovascular coupling and blood brain barrier in vitro. Cardiovasc Psychiatry Neurol 2011, 2011:646958.
18. Kuhn H.G., Dickinson-Anson H., Gage F.H. Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation. J Neurosci 1996, 16:2027-2033.
19. Kume S., Uzu T., Horiike K., Chin-Kanasaki M., Isshiki K., Araki S., et al. Calorie restriction enhances cell adaptation to hypoxia through Sirt1-dependent mitochondrial autophagy in mouse aged kidney. J Clin Invest 2010, 120:1043-1055.
20. Legradi A., Varszegi S., Szigeti C., Gulya K. Adult rat hippocampal slices as in vitro models for neurodegeneration: studies on cell viability and apoptotic processes. Brain Res Bull 2011, 84:39-44.
21. Li D., Field P.M., Starega U., Li Y., Raisman G. Entorhinal axons project to dentate gyrus in organotypic slice co-culture. Neuroscience 1993, 52:799-813.
22. Martinez R., Eraso D., Geffin R., McCarthy M. A two-culture method for exposure of human brain organotypic slice cultures to replicating human immunodeficiency virus type 1. J Neurosci Methods 2011, 200:74-79.
23. Mielke J.G., Comas T., Woulfe J., Monette R., Chakravarthy B., Mealing G.A. Cytoskeletal, synaptic, and nuclear protein changes associated with rat interface organotypic hippocampal slice culture development. Brain Res Dev Brain Res 2005, 160:275-286.
24. Muller D., Buchs P.A., Stoppini L. Time course of synaptic development in hippocampal organotypic cultures. Brain Res Dev Brain Res 1993, 71:93-100.
25. Nakayama G.R., Caton M.C., Nova M.P., Parandoosh Z. Assessment of the Alamar Blue assay for cellular growth and viability in vitro. J Immunol Methods 1997, 204:205-208.
26. Pena F. Organotypic cultures as tool to test long-term effects of chemicals on the nervous system. Curr Med Chem 2010, 17:987-1001.
27. Rao M.S., Hattiangady B., Abdel-Rahman A., Stanley D.P., Shetty A.K. Newly born cells in the ageing dentate gyrus display normal migration, survival and neuronal fate choice but endure retarded early maturation. Eur J Neurosci 2005, 21:464-476.
28. Riddle D.R., Lichtenwalner R.J. Neurogenesis in the adult and aging brain 2007.
29. Sadgrove M.P., Laskowski A., Gray W.P. Examination of granule layer cell count, cell density, and single-pulse BrdU incorporation in rat organotypic hippocampal slice cultures with respect to culture medium, septotemporal position, and time in vitro. J Comp Neurol 2006, 497:397-415.
30. Schrag M., Sharma S., Brown-Borg H., Ghribi O. Hippocampus of Ames dwarf mice is resistant to beta-amyloid-induced tau hyperphosphorylation and changes in apoptosis-regulatory protein levels. Hippocampus 2008, 18:239-244.
31. Schuff N., Woerner N., Boreta L., Kornfield T., Shaw L.M., Trojanowski J.Q., et al. MRI of hippocampal volume loss in early Alzheimer's disease in relation to ApoE genotype and biomarkers. Brain 2009, 132:1067-1077.
32. Staal J.A., Alexander S.R., Liu Y., Dickson T.D., Vickers J.C. Characterization of cortical neuronal and glial alterations during culture of organotypic whole brain slices from neonatal and mature mice. PLoS One 2011, 6:e22040.
33. Stoppini L., Buchs P.A., Muller D. A simple method for organotypic cultures of nervous tissue. J Neurosci Methods 1991, 37:173-182.
34. Su T., Paradiso B., Long Y.S., Liao W.P., Simonato M. Evaluation of cell damage in organotypic hippocampal slice culture from adult mouse: a potential model system to study neuroprotection. Brain Res 2011, 1385:68-76.
35. Sundstrom L., Morrison B., Bradley M., Pringle A. Organotypic cultures as tools for functional screening in the CNS. Drug Discov Today 2005, 10:993-1000.
36. Taylor L., Griffith W.H. Age-related decline in cholinergic synaptic transmission in hippocampus. Neurobiol Aging 1993, 14:509-515.
37. Ullrich C., Daschil N., Humpel C. Organotypic vibrosections: novel whole sagittal brain cultures. J Neurosci Methods 2011, 201:131-141.
38. Varghese K., Das M., Bhargava N., Stancescu M., Molnar P., Kindy M.S., et al. Regeneration and characterization of adult mouse hippocampal neurons in a defined in vitro system. J Neurosci Methods 2009, 177:51-59.
39. Xiang Z., Hrabetova S., Moskowitz S.I., Casaccia-Bonnefil P., Young S.R., Nimmrich V.C., et al. Long-term maintenance of mature hippocampal slices in vitro. J Neurosci Methods 2000, 98:145-154.