Addition of glutamate to serum-free culture promotes recovery of electrical activity in adult hippocampal neurons in vitro

Journal of Neuroscience Methods

Volumn 190, Issue 2, 2010, Pages 155-163

  Edwards, Darin ^a   Das, Mainak ^a   Molnar, Peter ^a   Hickman, James J ^a  

^a NanoScience Technology Center   (United States)

Author keywords

Adult; Culture; Electrophysiology; Hippocampal; Neuron

Indexed keywords

GLUTAMIC ACID; CULTURE MEDIUM; GLASS;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL SURVIVAL; CONTROLLED STUDY; CULTURE MEDIUM; ELECTROPHYSIOLOGY; EXCITOTOXICITY; HIPPOCAMPUS; NERVE CELL CULTURE; NERVE REGENERATION; NEUROPHYSIOLOGY; NONHUMAN; PRIORITY JOURNAL; RAT; TRAUMATIC BRAIN INJURY; AGING; ANIMAL; CULTURE TECHNIQUE; EXTRACELLULAR SPACE; IMMUNOHISTOCHEMISTRY; MEMBRANE POTENTIAL; METABOLISM; NERVE CELL; PATCH CLAMP TECHNIQUE; PHYSIOLOGY; PROCEDURES; SPRAGUE DAWLEY RAT; TIME;

AGING; ANIMALS; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CULTURE MEDIA, SERUM-FREE; EXTRACELLULAR SPACE; GLASS; GLUTAMIC ACID; HIPPOCAMPUS; IMMUNOHISTOCHEMISTRY; MEMBRANE POTENTIALS; NERVE REGENERATION; NEURONS; PATCH-CLAMP TECHNIQUES; RATS; RATS, SPRAGUE-DAWLEY; TIME FACTORS;

EID: 77954218953     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2010.04.030     Document Type: Article

References (38)

1. Auditore J.V., Wade L., Olson E.J. Occurrence of N-acetyl-l-glutamic acid in the human brain. J Neurochem 1966, 13(11):1149-1155.
2. Balazs R Trophic effect of glutamate. Curr Top Med Chem 2006, 6(10):961-968.
3. Barone F.C., White R.F., Spera P.A., Ellison J., Currie R.W., Wang X., et al. Ischemic preconditioning and brain tolerance: temporal histological and functional outcomes, protein synthesis requirement, and interleukin-1 receptor antagonist and early gene expression. Stroke 1998, 29(9):1937-1951.
4. Bousse L. Whole cell biosensors. Sens Actuators B-Chem 1996, 34(1-3):270-275.
5. Brewer G.J Isolation and culture of adult rat hippocampal neurons. J Neurosci Methods 1997, 71(2):143-155.
6. Brewer G.J. Age-related toxicity to lactate, glutamate, and beta-amyloid in cultured adult neurons. Neurobiol Aging 1998, 19(6):561-568.
7. Brewer G.J., Lim A., Capps N.G., Torricelli J.R. Age-related calcium changes, oxyradical damage, caspase activation and nuclear condensation in hippocampal neurons in response to glutamate and beta-amyloid. Exp Gerontol 2005, 40(5):426-437.
8. Brewer G.J., Torricelli J.R., Evege E.K., Price P.J. Optimized survival of hippocampal-neurons in B27-supplemented neurobasal(Tm), a new serum-free medium combination. J Neurosci Res 1993, 35(5):567-576.
9. Coceres A., Banker G., Steward O., Binder L., Payne M. MAP2 is localized to the dendrites of hippocampal neurons which develop in culture. Brain Res 1984, 315:314-318.
10. Dalva M.B., Takasu M.A., Lin M.Z., Shamah S.M., Hu L., Gale N.W., et al. EphB receptors interact with NMDA receptors and regulate excitatory synapse formation. Cell 2000, 103(6):945-956.
11. Daoudal G, Hanada Y., Debanne D. Bidirectional plasticity of excitatory postsynaptic potential (EPSP)-spike coupling in CA1 hippocampal pyramidal neurons. PNAS 2002, 99(22):14512-14517.
12. Das M., Bhargava N., Bhalkikar A., Kang J.-F., Hickman J.J. Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture. Exp Neurol 2008, 209:171-180.
13. Das M., Bhargava N., Gregory C., Riedel L., Molnar P., Hickman J.J. Adult rat spinal cord culture on an organosilane surface in a novel serum-free medium. In Vitro Cell Dev Biol-Anim 2005, 41(10):343-348.
14. Das M., Molnar P., Devaraj H., Poeta M., Hickman J. Electrophysiological and morphological characterization of rat embryonic motoneurons in a defined system. Biotechnol Prog 2003, 19:1756-1761.
15. Evans M.S., Collings M.A., Brewer G.J. Electrophysiology of embryonic, adult and aged rat hippocampal neurons in serum-free culture. J Neurosci Methods 1998, 79(1):37-46.
16. Ginsberg S.D Glutamatergic neurotransmission expression profiling in the mouse hippocampus after perforant-path transection. Am J Geriatr Psychiatry 2005, 13(12):1052-1061.
17. Harms H., Wells M.C., van der Meer J.R. Whole-cell living biosensors-are they ready for environmental application. Appl Microbiol Biotechnol 2006, 70(3):273-280.
18. Heiduschka P., Thanos S. Implantable bioelectronic interfaces for lost nerve functions. Prog Neurobiol 1998, 55(5):433-461.
19. Hickman J.J., Bhatia S.K., Quong J.N., Shoen P., Stenger D.A., Pike C.J., et al. Rational pattern design for in-vitro cellular networks using surface photochemistry. J Vac Sci Technol A-Vac Surf Films 1994, 12(3):607-616.
20. Husi H, Ward M.A., Choudhary J.S., Blackstock W.P., Grant S.G. Proteomic analysis of NMDA receptor-adhesion protein signaling complexes. Nat Neurosci 2000, 3(7):661-669.
21. Kornhauser J., Cowan C., Shaywitz A., Dolmetsch R., Griffith E., Hu L., et al. CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 2002, 34:221-233.
22. Mattson M.P. Neurotransmitters in the regulation of neuronal cytoarchitecture. Brain Res 1988, 472(2):179-212.
23. Mattson M.P., Dou P., Kater S.B. Outgrowth-regulating actions of glutamate in isolated hippocampal pyramidal neurons. J Neurosci 1988, 8(6):2087-2100.
24. Nakagawa S., Kim J., Lee R., Chen J., Fujioka T., Malberg J., et al. Localization of phosphorylated cAMP response element-binding protein in immature neurons of adult hippocampus. J Neurosci 2002, 22:9868-9876.
25. Ravenscroft M.S., Bateman K.E., Shaffer K.M., Schessler H.M., Jung D.R., Schneider T.W., et al. Developmental neurobiology implications from fabrication and analysis of hippocampal neuronal networks on patterned silane-modified surfaces. J Am Chem Soc 1998, 120(47):12169-12177.
26. Schaffner A.E., Barker J.L., Stenger D.A., Hickman J.J. Investigation of the factors necessary for growth of hippocampal neurons in a defined system. J Neurosci Methods 1995, 62(1-2):111-119.
27. Simpson M.L., Sayler G.S., Fleming J.T., Applegate B. Whole-cell biocomputing. Trends Biotechnol 2001, 19(8):317-323.
28. Sjostrom P.J., Rancz E.A., Roth A., Hausser M. Dendritic excitability and synaptic plasticity. Physiol Rev 2008, 88(2):769-840.
29. Spargo B.J., Testoff M.A., Nielsen T.B., Stenger D.A., Hickman J.J., Rudolph A.S. Spatially controlled adhesion, spreading, and differentiation of endothelial cells on self-assembled molecular monolayers. Proc Natl Acad Sci USA 1994, 91(23):11070-11074.
30. Stemple D.L., Anderson D.J. Isolation of a stem cell for neurons and glia from the mammalian neural crest. Cell 1992, 71(6):973-985.
31. Stenger D.A., Hickman J.J., Bateman K.E., Ravenscroft M.S., Ma W., Pancrazio J.J., et al. Microlithographic determination of axonal/dendritic polarity in cultured hippocampal neurons. J Neurosci Methods 1998, 82(2):167-173.
32. Stenger D.A., Pike C.J., Hickman J.J., Cotman C.W. Surface determinants of neuronal survival and growth on self-assembled monolayers in culture. Brain Res 1993, 630(1-2):136-147.
33. Tominaga-Yoshino K., Urakubo T., Okada M., Matsuda H., Ogura A. Repetitive induction of late-phase LTP produces long-lasting synaptic enhancement accompanied by synaptogenesis in cultured hippocampal slices. Hippocampus 2008, 18(3):281-293.
34. Verderio C., Coco S., Pravettoni E., Bacci A., Matteoli M. Synaptogenesis in hippocampal cultures. Cell Mol Life Sci 1999, 55:1448-1462.
35. Walmsley B., Berntson A., Leao R.N., Fyffe R.E.W. Activity-dependent regulation of synaptic strength and neuronal excitability in central auditory pathways. J Physiol 2006, 572(2):313-321.
36. West A, Chen W., Dalva M., Dolmetsch R., Kornhauser J., Shaywitz A., et al. Calcium regulation of neuronal gene expression. PNAS 2001, 98:11024-11031.
37. Zhang W., Linden D.J. The other side of the engram: experience-driven changes in neuronal intrinsic excitability. Nat Rev Neurosci 2003, 4(11):885-900.
38. Zhu D., Wu X., Strauss K.I., Lipsky R.H., Qureshi Z., Terhakopian A., et al. N-Methyl-d-aspartate and TrkB receptors protect neurons against glutamate excitotoxicity through an extracellular signal-regulated kinase pathway. J Neurosci Res 2005, 80(1):104-113.