Back to basics: An evaluation of NaOH and alternative rapid DNA extraction protocols for DNA barcoding, genotyping, and disease diagnostics from fungal and oomycete samples

Molecular Ecology Resources

Volumn 13, Issue 1, 2013, Pages 66-74

  Osmundson, Todd W ^a   Eyre, Catherine A ^a   Hayden, Katherine M ^a   Dhillon, Jaskirn ^a   Garbelotto, Matteo M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Microsatellite genotyping; Moorea Biocode Project; Phytophthora ramorum; Plant pathology; RDNA internal transcribed spacer; Sudden Oak Death

Indexed keywords

CETRIMIDE; CHELEX 100; CHLOROFORM; DNA; PHENOL; PROTEINASE K; RESIN; SODIUM HYDROXIDE;

ARTICLE; CHEMISTRY; COMPARATIVE STUDY; DNA BARCODING; EVALUATION; FAGACEAE; FUNGUS; GENETICS; GENOTYPE; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; METHODOLOGY; MICROBIOLOGY; NUCLEIC ACID AMPLIFICATION; PHYTOPHTHORA; PLANT LEAF; POLYNESIA;

CETRIMONIUM COMPOUNDS; CHLOROFORM; DNA; DNA BARCODING, TAXONOMIC; ENDOPEPTIDASE K; FAGACEAE; FUNGI; GENOTYPE; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PHENOL; PHYTOPHTHORA; PLANT LEAVES; POLYNESIA; RESINS, SYNTHETIC; SODIUM HYDROXIDE; SPECIMEN HANDLING;

FUNGI; LITHOCARPUS; OOMYCETES; PHYTOPHTHORA RAMORUM;

EID: 84870924172     PISSN: 1755098X     EISSN: 17550998     Source Type: Journal    
DOI: 10.1111/1755-0998.12031     Document Type: Article

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