In-gel phosphatase assay using non-denaturing two-dimensional electrophoresis

Journal of Biochemistry

Volumn 152, Issue 6, 2012, Pages 557-563

  Baba, Hiromi ^a   Masuda, Yukihiro ^a   Sueyoshi, Noriyuki ^a   Kameshita, Isamu ^a  

^a KAGAWA UNIVERSITY   (Japan)

Author keywords

fluorogenic substrate; in gel assay; isoelectric focusing; phosphatase assay; two dimensional electrophoresis

Indexed keywords

MERCAPTOETHANOL; PHOSPHATASE; PROTEIN; UREA;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; ISOELECTRIC FOCUSING; LIQUID; NONHUMAN; PH; PHYSICAL PHASE; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT; TWO DIMENSIONAL ELECTROPHORESIS;

ANIMALS; BRAIN; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; EMBRYO, NONMAMMALIAN; ENZYME ASSAYS; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; HYMECROMONE; ISOELECTRIC FOCUSING; MALE; PHOSPHORIC MONOESTER HYDROLASES; PROTEIN DENATURATION; RATS; RATS, WISTAR; TISSUE EXTRACTS; UREA; ZEBRAFISH; ZEBRAFISH PROTEINS;

EID: 84870879648     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvs099     Document Type: Article

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