Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and β-galactosidases

Analytical Biochemistry

Volumn 273, Issue 1, 1999, Pages 41-48

  Gee, Kyle R ^a   Sun, Wei Chuan ^a   Bhalgat, Mahesh K ^a   Upson, Rosalyn H ^a   Klaubert, Dieter H ^a   Latham, Katherine A ^a   Haugland, Richard P ^a  

^a MOLECULAR PROBES INC   (United States)

Author keywords

4 methyl umbelliferone; Enzyme assays; High thoughput screening; Phosphatase; galactosidase

Indexed keywords

ALKALINITY; FLUORESCENCE; SUBSTRATES;

4-METHYL-UMBELLIFERONE; ACID PHOSPHATASE ACTIVITIES; ALKALINE PHOSPHATASE ACTIVITY; ENZYME ASSAYS; FLUORINATED DERIVATIVES; GALACTOSIDASES; PHOSPHATASE SUBSTRATES; THOUGHPUT;

PHOSPHATASES;

ACID PHOSPHATASE; BETA GALACTOSIDASE; GLYCOSIDASE; HYMECROMONE; PHOSPHATASE; PHOSPHATE; PYRANOSIDE; UMBELLIFERONE DERIVATIVE;

ARTICLE; ASSAY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; FLUORESCENCE; PH; PRIORITY JOURNAL;

EID: 0033566587     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4202     Document Type: Article

References (18)

1. Robinson D. Biochem. Soc. Trans. 16:1988;11-16.
2. Fernley H. N., Walker P. G. Biochem. J. 97:1965;95-103.
3. Young D. C., Kingsley S. D., Ryan K. A., Dutko F. J. Anal. Biochem. 215:1993;24-30.
4. Manafi M., Kneifel W., Bascomb S. Microbiol. Rev. 55:1991;335-348.
5. Robinson D., Willcox P. Biochim. Biophys. Acta. 191:1969;183-186.
6. Rakhmanova V. A., MacDonald R. C. Anal. Biochem. 257:1998;234-237.
7. Labrousse H., Guesdon J.-L., Ragimbeau J., Avrameas S. J. Immunol. Methods. 48:1982;133-147.
8. Goodwin R. H., Kavanagh F. Arch. Biochem. Biophys. 27:1950;152-173.
9. Sun W.-C., Gee K. R., Haugland R. P. Bioorg. Med. Chem. Lett. 8:1998;3107-3110.
10. Tenu J. P., Viratelle O. M., Garnier J., Yon J. Eur. J. Biochem. 26:1971;363-370.
11. Avrameas S. J. Immunol. Methods. 150:1993;23-32.
12. Hornbeck P., Winston S. E., Fuller S. A. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. Short Protocols in Molecular Biology. 1992;11-5-11-17 Greene and Wiley, New York.
13. Huschtscha L. I., Lucibello F. C., Bodmer W. F. In Vitro Cell. Dev. Biol. 25:1989;105-108.
14. Vlieger A. M., Medenblik A. M. J. C., van Gijlswijk R. P. M., Tanke H. J., van der Ploeg M., Gratama J. W., Raap A. K. Anal. Biochem. 205:1992;1-7.
15. Price J., Turner D., Cepko C. Proc. Natl. Acad. Sci. USA. 84:1987;156-160.
16. Galjaard H., Reuser A. J. Dingle J. T., Dean R. T., Sly W. Lysosomes in Biology and Pathology. 1984;315-345 Elsevier Science, Amsterdam.
17. Annunziata P., Di Matteo G. Clin. Chim. Acta. 90:1978;101-106.
18. Luyten P. M., Hoogeveen A. T., Galjaard H. J. Histochem. Cytochem. 33:1985;965-968.