High yield expression of serine/threonine protein phosphatase type 5, and a fluorescent assay suitable for use in the detection of catalytic inhibitors

Assay and Drug Development Technologies

Volumn 5, Issue 5, 2007, Pages

  Ni, Li ^a   Swingle, Mark S ^a   Bourgeois, Austin C ^a   Honkanen, Richard E ^a  

^a UNIVERSITY OF SOUTH ALABAMA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHOSERINE; PHOSPHOTHREONINE; SERINE PHOSPHATASE TYPE 5; THREONINE PHOSPHATASE TYPE 5; UNCLASSIFIED DRUG; 4 METHYLUMBELLIFERYL PHOSPHATE; 4 NITROPHENYLPHOSPHATASE; 4-METHYLUMBELLIFERYL PHOSPHATE; DRUG DERIVATIVE; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; ENZYME INHIBITOR; FLUORESCENT DYE; HYMECROMONE; NUCLEAR PROTEIN; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN PHOSPHATASE 5;

ARTICLE; CATALYSIS; ENZYME ASSAY; FLUORESCENCE ANALYSIS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TITRIMETRY; BIOSYNTHESIS; CHEMISTRY; DRUG ANTAGONISM; ESCHERICHIA COLI; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; SPECTROFLUOROMETRY;

4-NITROPHENYLPHOSPHATASE; CATALYSIS; ENZYME INHIBITORS; ESCHERICHIA COLI; FLUORESCENT DYES; HUMANS; HYMECROMONE; INDICATORS AND REAGENTS; NUCLEAR PROTEINS; PHOSPHOPROTEIN PHOSPHATASES; SPECTROMETRY, FLUORESCENCE;

EID: 35349019372     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2007.079     Document Type: Article

References (27)

1. Gentile S, Darden T, Erxleben C, Romeo C, Russo A, Martin N, et al.: Rac GTPase signaling through the PP5 protein phosphatase. Proc Natl Acad Sci USA 2006;103:5202-5206.
2. Fu Z, Larson KA, Chitta RK, Parker SA, Turk BE, Lawrence MW, et al.: Identification of yin-yang regulators and a phosphorylation consensus for male germ cell-associated kinase (MAK)-related kinase. Mol Cell Biol 2006;26:8639-8654.
3. Partch CL, Shields KF, Thompson CL, Selby CP, Sancar A: Posttranslational regulation of the mammalian circadian clock by cryptochrome and protein phosphatase 5. Proc Natl Acad Sci U S A 2006;103:10467-10472.
4. Zhang J, Bao S, Furumai R, Kucera KS, Ali A, Dean NM, et al.: Protein phosphatase 5 is required for ATR-mediated checkpoint activation. Mol Cell Biol 2005;25:9910-9919.
5. Zhou G, Golden T, Aragon IV, Honkanen RE: Ser/Thr protein phosphatase 5 inactivates hypoxia-induced activation of an apoptosis signal-regulating kinase 1/MKK-4/JNK signaling cascade. J Biol Chem 2004;279:46595-46605.
6. von Kriegsheim A, Pitt A, Grindlay GJ, Kolch W, Dhillon AS: Regulation of the Raf-MEK-ERK pathway by protein phosphatase 5. Nat Cell Biol 2006;8:1011-1016.
7. Urban G, Golden T, Aragon IV, Cowsert L, Cooper SR, Dean NM, et al.: Identification of a functional link for the p53 tumor suppressor protein in dexamethasone-induced growth suppression. J Biol Chem 2003;278:9747-9753.
8. Honkanen RE: Cantharidin, another natural toxin that inhibits the activity of serine/threonine protein phosphatases types 1 and 2A. FEBS Lett 1993;330:283-286.
9. Honkanen RE, Zwiller J, Moore RE, Daily SL, Khatra BS, Dukelow M, et al.: Characterization of microcystin-LR, a potent inhibitor of type 1 and type 2A protein phosphatases. J Biol Chem 1990;265:19401-19404.
10. Walsh AH, Cheng A, Honkanen RE: Fostriecin, an antitumor antibiotic with inhibitory activity against serine/threonine protein phosphatases types 1 (PP1) and 2A (PP2A), is highly selective for PP2A. FEBS Lett 1997;416:230-234.
11. Huang X, Swingle MR, Honkanen RE: Photoreceptor serine/threonine protein phosphatase type 7: cloning, expression, and functional analysis. Methods Enzymol 2000;315:579-593.
12. Honkanen RE, Dukelow M, Zwiller J, Moore RE, Khatra BS, Boynton AL: Cyanobacterial nodularin is a potent inhibitor of type 1 and type 2A protein phosphatases. Mol Pharmacol 1991;40:577-583.
13. Zhang JH, Chung TD, Oldenburg KR: A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999;4:67-73.
14. Wechsler T, Chen BP, Harper R, Morotomi-Yano K, Huang BC, Meek K, et al.: DNA-PKcs function regulated specifically by protein phosphatase 5. Proc Natl Acad Sci USA 2004;101:1247-1252.
15. Kurose H: Galpha12 and Galpha13 as key regulatory mediator in signal transduction. Life Sci 2003;74:155-161.
16. Chinkers M: Protein phosphatase 5 in signal transduction. Trends Endocrinol Metab 2001;12:28-32.
17. Morita K, Saitoh M, Tobiume K, Matsuura H, Enomoto S, Nishitoh H, et al.: Negative feedback regulation of ASK1 by protein phosphatase 5 (PP5) in response to oxidative stress. EMBO J 2001;20:6028-6036.
18. Silverstein AM, Galigniana MD, Chen MS, Owens-Grillo JK, Chinkers M, Pratt WB: Protein phosphatase 5 is a major component of glucocorticoid receptor.hsp90 complexes with properties of an FK506-binding immunophilin. J Biol Chem 1997;272:16224-16230.
19. Swingle MR, Honkanen RE, Ciszak EM: Structural basis for the catalytic activity of human serine/threonine protein phosphatase-5. J Biol Chem 2004;279:33992-33999.
20. Yang J, Roe SM, Cliff MJ, Williams MA, Ladbury JE, Cohen PT, et al.: Molecular basis for TPR domain-mediated regulation of protein phosphatase 5. EMBO J 2005;24:1-10.
21. Cliff MJ, Williams MA, Brooke-Smith J, Barford D, Ladbury JE: Molecular recognition via coupled folding and binding in a TPR domain. J Mol Biol 2005;346:717-732.
22. Chen MS, Silverstein AM, Pratt WB, Chinkers M: The tetratricopeptide repeat domain of protein phosphatase 5 mediates binding to glucocorticoid receptor heterocomplexes and acts as a dominant negative mutant. J Biol Chem 1996;271:32315-32320.
23. Honkanen RE, Golden T: Regulators of serine/threonine protein phosphatases at the dawn of a clinical era? Curr Med Chem 2002;9:2055-2075.
24. Gee KR, Sun WC, Bhalgat MK, Upson RH, Klaubert DH, Latham KA, et al.: Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases. Anal Biochem 1999;273:41-48.
25. Egloff MP, Cohen PT, Reinemer P, Barford D: Crystal structure of the catalytic subunit of human protein phosphatase 1 and its complex with tungstate. J Mol Biol 1995;254:942-959.
26. Kita A, Matsunaga S, Takai A, Kataiwa H, Wakimoto T, Fusetani N, et al.: Crystal structure of the complex between calyculin A and the catalytic subunit of protein phosphatase 1. Structure 2002;10:715-724.
27. Xing Y, Xu Y, Chen Y, Jeffrey PD, Chao Y, Lin Z, et al.: Structure of protein phosphatase 2A core enzyme bound to tumor-inducing toxins. Cell 2006;127:341-353.