Riboflavin accumulation and characterization of cDNAs encoding lumazine synthase and riboflavin synthase in bitter melon (Momordica charantia)

Journal of Agricultural and Food Chemistry

Volumn 60, Issue 48, 2012, Pages 11980-11986

  Tuan, Pham Anh ^a   Kim, Jae Kwang ^b   Lee, Sanghyun ^c   Chae, Soo Cheon ^d   Park, Sang Un ^a  

^a Chungnam National University   (South Korea)

^b National Institute of Animal Science   (South Korea)

^c Chung Ang University   (South Korea)

^d Kongju National University   (South Korea)

Author keywords

characterization; cloning; lumazine synthase; Momordica charantia; riboflavin synthase; vitamin B2

Indexed keywords

AMINO ACID SEQUENCE; BIOSYNTHETIC PATHWAY; BITTER MELON; EXPRESSION ANALYSIS; EXPRESSION LEVELS; FLAVIN MONONUCLEOTIDES; FRUIT RIPENING; HPLC ANALYSIS; LAST STAGE; LUMAZINE SYNTHASE; METABOLIC ENZYMES; MOMORDICA CHARANTIA; N-TERMINAL EXTENSION; ORTHOLOGOUS GENES; PCR APPROACH; SEQUENCE IDENTITY; SYNTHASES; VEGETABLE CROPS; VITAMIN B;

AGRICULTURE; AMINO ACIDS; CHARACTERIZATION; ENCODING (SYMBOLS); ENZYMES; PLANTS (BOTANY);

CLONING;

6,7 DIMETHYL 8 RIBITYLLUMAZINE SYNTHASE; 6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE; COMPLEMENTARY DNA; MULTIENZYME COMPLEX; RIBOFLAVIN; RIBOFLAVIN SYNTHASE; VEGETABLE PROTEIN;

3' UNTRANSLATED REGION; AMINO ACID SEQUENCE; ARTICLE; ENZYMOLOGY; FRUIT; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOMORDICA CHARANTIA; PHYSIOLOGY; PLANT LEAF; PLANT ROOT; SEQUENCE HOMOLOGY;

3' UNTRANSLATED REGIONS; AMINO ACID SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; FRUIT; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; MOLECULAR SEQUENCE DATA; MOMORDICA CHARANTIA; MULTIENZYME COMPLEXES; PLANT LEAVES; PLANT PROTEINS; PLANT ROOTS; RIBOFLAVIN; RIBOFLAVIN SYNTHASE; SEQUENCE HOMOLOGY, AMINO ACID;

ANIMALIA; CUCUMIS; MOMORDICA CHARANTIA;

EID: 84870682776     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf3036963     Document Type: Article

References (34)

1. Gregory, J. F. I. Nutritional properties and significance of vitamin glycosides Annu. Rev. Nutr. 1998, 18, 277-296
2. Gastaldi, G.; Laforenza, U.; Gasirola, D.; Ferrari, G.; Tosco, M.; Rindi, G. Energy depletion differently affects membrane transport nd intracellular metabolism of riboflavin taken up by isolated rat enterocytes J. Nutr. 1999, 129, 406-409
3. Massey, V. The chemical and biological versatility of riboflavin Biochem. Soc. Trans. 2000, 28, 283-296
4. de Souza, A. C.; Kodach, L.; Gadelha, F. R.; Bos, C. L.; Cavagis, A. D.; Aoyama, H.; Peppelenbosch, M. P.; Ferreira, C. V. A promising action of riboflavin as a mediator of leukaemia cell death Apoptosis 2006, 11, 1761-1771
5. Powers, H. J. Riboflavin (vitamin B-2) and health Am. J. Clin. Nutr. 2003, 77, 1352-1360
6. Dong, H.; Beer, S. V. Riboflavin induces disease resistance in plants by activating a novel signal transduction pathway Phytopathology 2000, 90, 801-811
7. Taheri, P.; Höfte, M. Riboflavin induces resistance in rice against Rhizoctonia sheath diseases by activating signal transduction pathways leading to upregulation of rice cationic peroxidase and formation of lignin as a structural barrier Commun. Agric. Appl. Biol. Sci. 2006, 71, 255-258
8. Hu, Y.-S.; Zhang, L.; Di, P.; Chen, W.-S. Cloning and induction of phenylalanine ammonia-lyase gene from Salvia miltiorrhiza and its effect on hydrophilic phenolic acids levels Chinese J. Nat. Med. 2009, 7 (6) 449-457
9. Wu, T.; Guo, A.; Zhao, Y.; Wang, X.; Wang, Y.; Zhao, D.; Li, X.; Ren, H.; Dong, H. Ectopic expression of the rice lumazine synthase gene contributes to defense responses in transgenic tobacco Phytopathology 2010, 100, 573-581
10. Fischer, M.; Bacher, A. Biosynthesis of vitamin B2: Structure and mechanism of riboflavin synthase Arch. Biochem. Biophys. 2008, 474, 252-265
11. Jordan, D. B.; Bacot, K. O.; Carlson, T. J.; Kessel, M.; Viitanen, P. V. Plant riboflavin biosynthesis. Cloning, chloroplast localization, expression, purification, and partial characterization of spinach lumazine synthase J. Biol. Chem. 1999, 274, 22114-22121
12. Schramek, N.; Haase, I.; Fischer, M.; Bacher, A. Biosynthesis of riboflavin. Single turnover kinetic analysis of 6,7-dimethyl-8-ribityllumazine synthase J. Am. Chem. Soc. 2003, 125, 4460-4466
13. Illarionov, B.; Haase, I.; Bacher, A.; Fischer, M.; Schramek, N. Presteady state kinetic analysis of riboflavin synthase J. Biol. Chem. 2003, 278, 47700-47706
14. Stahmann, K. P.; Revuelta, J. L.; Seulberger, H. Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production Appl. Microbiol. Biotechnol. 2000, 53, 509-516
15. Fischer, M.; Bacher, A. Biosynthesis of vitamin B2 in plants Physiol. Plantarum 2006, 126, 304-318
16. Platel, K.; Srinivasan, K. Plant foods in the management of diabetes mellitus: vegetables as potential hypoglycemic agents Nahrung 1997, 41, 68-74
17. Zhang, M.; Hettiarachchy, N. S.; Horax, R.; Chen, P.; Over, K. F. Effect of maturity stages and drying methods on the retention of selected nutrients and phytochemicals in bitter melon (Momordica charantia) leaf J. Food Sci. 2009, 74, 441-448
18. Raman, A.; Lau, C. Anti-diabetic properties and phytochemistry of Momordica charantia L (Cucurbitaceae) Phytomedicine 1996, 2, 349-362
19. Grover, J. K.; Yadav, S. P. Pharmacological actions and potential uses of Momordica charantia: a review J. Ethnopharmacol. 2004, 93, 123-132
20. Gurbuz, I.; Akyuz, C.; Yesilada, E.; Bilge, S. Anti-ulcerogenic effect of Momordica charantia L. fruits on various ulcer models in rats J. Ethnopharmacol. 2000, 71, 77-82
21. Nerurkar, P.; Ray, R. Bitter melon: antagonist to cancer Pharm. Res. 2010, 27, 1049-1053
22. Esteve, M. J.; Farré, R.; Frígola, A.; García- Cantabella, J. M. Simultaneous determination of thiamin and riboflavin in mushrooms by liquid chromatography J. Agric. Food Chem. 2001, 49, 1450-1454
23. Fischer, M.; Romisch, W.; Saller, S.; Illarionov, B.; Richter, G.; Rohdich, F.; Eisenreich, W.; Bacher, A. Evolution of vitamin B2 biosynthesis: structural and functional similarity between pyrimidine deaminases of eubacterial and plant origin J. Biol. Chem. 2004, 279, 36299-36308
24. Gerhardt, S.; Schott, A. K.; Kairies, N.; Cushman, M.; Illarionov, B.; Eisenreich, W.; Bacher, A.; Huber, R.; Steinbacher, S.; Fischer, M. Studies on the reaction mechanism of riboflavin synthase: X-ray crystal structure of a complex with 6-carboxyethyl-7-oxo-8-ribityllumazine Structure 2002, 10, 1371-1381
25. Fischer, M.; Haase, I.; Feicht, R.; Schramek, N.; Köhler, P.; Schieberle, P.; Bacher, A. Evolution of vitamin B2 biosynthesis: riboflavin synthase of Arabidopsis thaliana and its inhibition by riboflavin Biol. Chem. 2005, 386, 417-428
26. Roje, S. Vitamin B biosynthesis in plants Phytochemistry 2007, 68, 1904-1921
27. Robinson, N. J.; Procter, C. M.; Connolly, E. L.; Guerinot, M. L. A ferric-chelate reductase for iron uptake from soils Nature 1999, 397, 694-697
28. Rodríguez-Celma, J.; Lattanzio, G.; Grusak, M. A.; Abadía, A.; Abadía, J.; López-Millán, A. F. Root responses of Medicago truncatula plants grown in two different iron deficiency conditions: changes in root protein profile and riboflavin biosynthesis J. Proteome Res. 2011, 10, 2590-2601
29. Mori, T.; Sakurai, E. Riboflavin affects anthocyanin synthesis in nitrogen culture using strawberry suspended cells J. Food Sci. 1996, 61, 698-702
30. Tuan, P. A.; Kim, J. K.; Park, N. I.; Lee, S. Y.; Park, S. U. Carotenoid content and expression of phytoene synthase and phytoene desaturase genes in bitter melon (Momordica charantia) Food Chem. 2011, 126, 1686-1692
31. Botella-Pavía, P.; Rodríguez-Concepción, M. Carotenoid biotechnology in plants for nutritionally improved foods Physiol. Plant. 2006, 126, 369-381
32. Kritchevsky, S. B. β-Carotene, carotenoids and the prevention of coronary heart disease J. Nutr. 1999, 129 (1) 5-8
33. Perkins, J. B.; Pero, J. G.; Sloma, A. Riboflavin overproducing strains of bacteria. Eur. Patent Appl. EP0405370, 1991.
34. Heefner, D. L.; Weaver, C. A.; Yarus, M. J.; Burdzinski, L. A. Method for producing riboflavin with Candida famata. U.S. Patent 5164303, 1992.