Brief report: Misinterpretation of coculture differentiation experiments by unintended labeling of cardiomyocytes through secondary transduction: Delusions and solutions

Stem Cells

Volumn 30, Issue 12, 2012, Pages 2830-2834

  Ramkisoensing, Arti A ^a   De Vries, Antoine A F ^a   Schalij, Martin J ^a   Atsma, Douwe E ^a   Pijnappels, Daniël A ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Cardiomyocyte; Differentiation; Lentiviral vector; Neutralizing activity; Stem cell

Indexed keywords

ALPHA ACTININ; ENHANCED GREEN FLUORESCENT PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; LAMIN A; LAMIN C; LENTIVIRUS VECTOR;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL LABELING; COCULTURE; CONTROLLED STUDY; GENE; GENETIC TRANSDUCTION; GFP GENE; HEART MUSCLE CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; NEWBORN; NONHUMAN; PSEUDOTYPING; RAT; STEM CELL; VESICULAR STOMATITIS VIRUS; VIRAL GENE DELIVERY SYSTEM;

ANIMALS; CELL DIFFERENTIATION; COCULTURE TECHNIQUES; GENETIC VECTORS; HUMANS; LENTIVIRUS; MYOCYTES, CARDIAC; RABBITS; RATS; TRANSDUCTION, GENETIC;

ORYCTOLAGUS CUNICULUS; RATTUS; VESICULAR STOMATITIS VIRUS;

EID: 84870387629     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1002/stem.1236     Document Type: Article

References (20)

1. Pijnappels DA, Schalij MJ, Atsma DE. Response to the letter by Rose et al. Circ Res 2009;104:e8.
2. Rose RA, Keating A, Backx PH. Do mesenchymal stromal cells trans-differentiate into functional cardiomyocytes? Circ Res 2008;103:e120.
3. Koninckx R, Hensen K, Daniels A et al. Human bone marrow stem cells co-cultured with neonatal rat cardiomyocytes display limited cardiomyogenic plasticity. Cytotherapy 2009;11:778-792.
4. Rose RA, Jiang H, Wang X et al. Bone marrow-derived mesenchymal stromal cells express cardiac-specific markers, retain the stromal phenotype, and do not become functional cardiomyocytes in vitro. Stem Cells 2008;26:2884-2892.
5. Kodama H, Inoue T, Watanabe R et al. Cardiomyogenic potential of mesenchymal progenitors derived from human circulating CD14+ monocytes. Stem Cells Dev 2005;14:676-686.
6. Nishiyama N, Miyoshi S, Hida N et al. The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro. Stem Cells 2007;25:2017-2024.
7. Numasawa Y, Kimura T, Miyoshi S et al. Treatment of human mesenchymal stem cells with angiotensin receptor blocker improved efficiency of cardiomyogenic transdifferentiation and improved cardiac function via angiogenesis. Stem Cells 2011;29:1405-1414.
8. Orlandi A, Pagani F, Avitabile D et al. Functional properties of cells obtained from human cord blood CD34+ stem cells and mouse cardiac myocytes in coculture. Am J Physiol Heart Circ Physiol 2008;294:H1541-H1549.
9. Pijnappels DA, Schalij MJ, Ramkisoensing AA et al. Forced alignment of mesenchymal stem cells undergoing cardiomyogenic differentiation affects functional integration with cardiomyocyte cultures. Circ Res 2008;103:167-176.
10. Shinmura D, Togashi I, Miyoshi S et al. Pretreatment of human mesenchymal stem cells with pioglitazone improved efficiency of cardiomyogenic transdifferentiation and cardiac function. Stem Cells 2011;29:357-366.
11. Tsuji H, Miyoshi S, Ikegami Y et al. Xenografted human amniotic membrane-derived mesenchymal stem cells are immunologically tolerated and transdifferentiated into cardiomyocytes. Circ Res 2010;106:1613-1623.
12. Pan YW, Scarlett JM, Luoh TT et al. Prolonged adherence of human immunodeficiency virus-derived vector particles to hematopoietic target cells leads to secondary transduction in vitro and in vivo. J Virol 2007;81:639-649.
13. Beebe DP, Cooper NR. Neutralization of vesicular stomatitis virus (VSV) by human complement requires a natural IgM antibody present in human serum. J Immunol 1981;126:1562-1568.
14. National Institutes of Health. Guide for the Care and Use of Laboratory Animals, 8th ed. National Academies Press, 2011.
15. Seppen J, Rijnberg M, Cooreman MP et al. Lentiviral vectors for efficient transduction of isolated primary quiescent hepatocytes. J Hepatol 2002;36:459-465.
16. van Tuyn J, Pijnappels DA, de Vries AA et al. Fibroblasts from human postmyocardial infarction scars acquire properties of cardiomyocytes after transduction with a recombinant myocardin gene. FASEB J 2007;21:3369-3379.
17. Ramkisoensing AA, Pijnappels DA, Askar SF et al. Human embryonic and fetal mesenchymal stem cells differentiate toward three different cardiac lineages in contrast to their adult counterparts. PLoS One 2011;6:e24164.
18. Ramkisoensing AA, Pijnappels DA, Swildens J et al. Gap junctional coupling with cardiomyocytes is necessary but not sufficient for cardiomyogenic differentiation of cocultured human mesenchymal stem cells. Stem Cells 2012;30:1236-1245.
19. Askar SF, Bingen BO, Swildens J et al. Connexin43 silencing in myofibroblasts prevents arrhythmias in myocardial cultures: Role of maximal diastolic potential. Cardiovasc Res 2012;93:434-444.
20. Burghoff S, Ding Z, Godecke S et al. Horizontal gene transfer from human endothelial cells to rat cardiomyocytes after intracoronary transplantation. Cardiovasc Res 2008;77:534-543.