Fibroblasts from human postmyocardial infarction scars acquire properties of cardiomyocytes after transduction with a recombinant myocardin gene

FASEB Journal

Volumn 21, Issue 12, 2007, Pages 3369-3379

  Van Tuyn, John ^a,b   Pijnappels, Daniël A ^a   De Vries, Antoine A F ^a   De Vries, Ingrid ^a   Van Der Velde van Dijke, Ietje ^a   Knaän Shanzer, Shoshan ^a   Van Der Laarse, Arnoud ^a   Schalij, Martin J ^a   Atsma, Douwe E ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b ROYAL NETHERLANDS ACADEMY OF ARTS AND SCIENCES   (Netherlands)

Author keywords

Conduction (block); Fibrosis; Gene transfer; Heart infarction; Myocytes

Indexed keywords

BIOLOGICAL MARKER; ION CHANNEL; LENTIVIRUS VECTOR; MYOCARDIN; TRANSCRIPTION FACTOR; NUCLEAR PROTEIN; TRANSACTIVATOR PROTEIN;

ACTION POTENTIAL; ADIPOGENESIS; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CONNEXIN 43 GENE; CONTROLLED STUDY; ELECTRIC CONDUCTIVITY; FIBROBLAST; GATA4 GENE; GENE; GENE EXPRESSION; GENE TRANSFER; HEART FUNCTION; HEART INFARCTION; HEART LEFT VENTRICLE; HEART MUSCLE CELL; HEART MUSCLE COMPLIANCE; HEART MUSCLE FIBROSIS; HEART MUSCLE INJURY; HEART PERFORMANCE; HEART VENTRICLE REMODELING; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VIVO STUDY; MYOCARDIN GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; RAT; RECOMBINANT GENE; SARCOMERE; SCAR FORMATION; SMOOTH MUSCLE; ANIMAL; BONE DEVELOPMENT; CELL CULTURE; CYTOLOGY; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSDUCTION; GENETICS; HEART MUSCLE; HEART MUSCLE CONDUCTION SYSTEM; HEART VENTRICLE; HELA CELL; LENTIVIRINAE; METABOLISM; PATHOLOGY; PHYSIOLOGY; PROMOTER REGION; SCAR;

LENTIVIRUS;

ADIPOGENESIS; ANIMALS; BIOLOGICAL MARKERS; CELL DIFFERENTIATION; CELLS, CULTURED; CICATRIX; ELECTRIC CONDUCTIVITY; FIBROBLASTS; GENE EXPRESSION REGULATION; GENETIC VECTORS; HEART CONDUCTION SYSTEM; HEART VENTRICLES; HELA CELLS; HUMANS; LENTIVIRUS; MYOCARDIAL INFARCTION; MYOCARDIUM; MYOCYTES, CARDIAC; NUCLEAR PROTEINS; OSTEOGENESIS; PHENOTYPE; PROMOTER REGIONS (GENETICS); RATS; TRANS-ACTIVATORS; TRANSCRIPTION FACTORS; TRANSDUCTION, GENETIC;

EID: 35248868915     PISSN: 08926638     EISSN: None     Source Type: Journal    
DOI: 10.1096/fj.07-8211com     Document Type: Article

References (43)

1. Frangogiannis, N. G. (2006) The mechanistic basis of infarct healing. Antioxid. Redox Signal. 8, 1907-1939
2. Sun, Y., Kiani, M. F., Postlethwaite, A. E., and Weber, K. T. (2002) Infarct scar as living tissue. Basic Res. Cardiol. 97, 343-347
3. Swynghedauw, B. (1999) Molecular mechanisms of myocardial remodeling. Physiol. Rev. 79, 215-262
4. Dimmeler, S., Zeiher, A. M., and Schneider, M. D. (2005) Unchain my heart: the scientific foundations of cardiac repair. J. Clin. Invest. 115, 572-583
5. De Muinck, E. D., Thompson, C., and Simons, M. (2005) Progress and prospects: cell based regenerative therapy for cardiovascular disease. Gene Ther. 13, 659-671
6. Chien, K. R. (2006) Lost and found: cardiac stem cell therapy revisited. J. Clin. Invest. 116, 1838-1840
7. Murry, C. E., Reinecke, H., and Pabon, L. M. (2006) Regeneration gaps. Observations on stem cells and cardiac repair. J. Am. Coll. Cardiol. 47, 1777-1785
8. Brown, R. D., Ambler, S. K., Mitchell, M. D., and Long, C. S. (2005) The cardiac fibroblast: therapeutic target in myocardial remodeling and failure. Annu. Rev. Pharmacol. Toxicol. 45, 657-687
9. Etzion, S., Barbash, I. M., Feinberg, M. S., Zarin, P., Miller, L., Guetta, E., Holbova, R., Kloner, R. A., Kedes, L. H., and Leor, J. (2002) Cellular cardiomyoplasty of cardiac fibroblasts by adenoviral delivery of MyoD ex vivo: an unlimited source of cells for myocardial repair. Circulation 106, I125-130
10. Kizana, E., Ginn, S. L., Allen, D. G., Ross, D. L., and Alexander, I. E. (2005) Fibroblasts can be genetically modified to produce excitable cells capable of electrical coupling. Circulation 111, 394-398
11. Murry, C. E., Kay, M. A., Bartosek, T., Hauschka, S. D., and Schwartz, S. M. (1996) Muscle differentiation during repair of myocardial necrosis in rats via gene transfer with MyoD. J. Clin. Invest. 98, 2209-2217
12. Hille, B. (2001) Ion Channels of Excitable Membranes, Sinauer Associates, Inc., Sunderland, Massachusetts
13. Dunwoodie, S. L. (2007) Combinatorial signaling in the heart orchestrates cardiac induction, lineage specification and chamber formation. Semin. Cell. Dev. Biol. 18, 54-66
14. Srivastava, D. (2006) Making or breaking the heart: from lineage determination to morphogenesis. Cell 126, 1037-1048
15. Fukushige, T., Brodigan, T. M., Schriefer, L. A., Waterston, R. H., and Krause, M. (2006) Defining the transcriptional redundancy of early bodywall muscle development in C. elegans: evidence for a unified theory of animal muscle development. Genes Dev. 20, 3395-3406
16. Wang, D.-Z., Chang, P. S., Wang, Z., Sutherland, L., Richardson, J. A., Small, E., Krieg, P. A., and Olson, E. N. (2001) Activation of cardiac gene expression by myocardin, a transcriptional cofactor for serum response factor. Cell 105, 851-862
17. Chen, J., Kitchen, C. M., Streb, J. W., and Miano, J. M. (2002) Myocardin: a component of a molecular switch for smooth muscle differentiation. J. Mol. Cell. Cardiol. 34, 1345-1356
18. Creemers, E. E., Sutherland, L. B., Oh, J., Barbosa, A. C., and Olson, E. N. (2006) Coactivation of MEF2 by the SAP domain proteins myocardin and MASTR. Mol. Cell. 23, 83-96
19. van Tuyn, J., Knaan-Shanzer, S., van de Watering, M. J., de Graaf, M., van der Laarse, A., Schalij, M. J., van der Wall, E. E., de Vries, A. A., and Atsma, D. E. (2005) Activation of cardiac and smooth muscle-specific genes in primary human cells after forced expression of human myocardin. Cardiovasc. Res. 67, 245-255
20. Van Tuyn, J., Atsma, D. E., Winter, E. M., van der Velde-van Dijke, I., Pijnappels, D. A., Bax, N. A., Knaan-Shanzer, S., Gittenberger-de Groot, A. C., Poelmann, R. E., et al. (2006) Epicardial cells of human adults can undergo an epithelial-tomesenchymal transition and obtain characteristics of smooth muscle cells in vitro. Stem Cells 25, 271-278
21. Pijnappels, D. A., Schalij, M. J., van Tuyn, J., Ypey, D. L., de Vries, A. A., van der Wall, E. E., van der Laarse, A., and Atsma, D. E. (2006) Progressive increase in conduction velocity across human mesenchymal stem cells is mediated by enhanced electrical coupling. Cardiovasc. Res. 72, 282-291
22. Niwa, H., Yamamura, K., and Miyazaki, J. (1991) Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108, 193-199
23. Vellinga, J., Uil, T. G., de Vrij, J., Rabelink, M. J., Lindholm, L., and Hoeben, R. C. (2006) A system for efficient generation of adenovirus protein IX-producing helper cell lines. J. Gene Med. 8, 147-154
24. Donello, J. E., Loeb, J. E., and Hope, T. J. (1998) Woodchuck hepatitis virus contains a tripartite posttranscriptional regulatory element. J. Virol. 72, 5085-5092
25. Graham, F. L., and van der Eb, A. J. (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52, 456-467
26. Pfaffl, M. W., Horgan, G. W., and Dempfle, L. (2002) Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 30, e36
27. Parmacek, M. S., and Solaro, R. J. (2004) Biology of the troponin complex in cardiac myocytes. Prog. Cardiovasc. Dis. 47, 159-176
28. Baroldi, G., Silver, M. D., De Maria, R., Parodi, O., and Pellegrini, A. (1997) Lipomatous metaplasia in left ventricular scar. Can. J. Cardiol. 13, 65-71
29. Winer-Muram, H. T., Tann, M., Aisen, A. M., Ford, L., Jennings, S. G., and Bretz, R. (2004) Computed tomography demonstration of lipomatous metaplasia of the left ventricle following myocardial infarction. J. Comput. Assist. Tomogr. 28, 455-458
30. Camelliti, P., Green, C. R., and Kohl, P. (2006) Structural and functional coupling of cardiac myocytes and fibroblasts. Adv. Cardiol. 42, 132-149
31. Mitchell, J. A., Shynlova, O., Langille, B. L., and Lye, S. J. (2004) Mechanical stretch and progesterone differentially regulate activator protein-1 transcription factors in primary rat myometrial smooth muscle cells. Am. J. Physiol. 287, E439-E445
32. Reiser, P. J., Westfall, M. V., Schiaffino, S., and Solaro, R. J. (1994) Tension production and thin-filament protein isoforms in developing rat myocardium. Am. J. Physiol. 267, H1589-H1596
33. Huang, X. P., Pi, Y. Q., Lee, K. J., Henkel, A. S., Gregg, R. G., Powers, P. A., and Walker, J. W. (1999) Cardiac troponin I gene knockout: a mouse model of myocardial troponin I deficiency. Circ. Res. 84, 1-8
34. Takemura, H., Yasui, K., Opthof, T., Niwa, N., Horiba, M., Shimizu, A., Lee, J. K., Honjo, H., Kamiya, K., Ueda, Y., and Kodama, I. (2005) Subtype switching of L-type Ca2 channel from Cav1.3 to Cav1.2 in embryonic murine ventricle. Circ. J. 69, 1405-1411
35. Yasui, K., Liu, W., Opthof, T., Kada, K., Lee, J. K., Kamiya, K., and Kodama, I. (2001) I(f) current and spontaneous activity in mouse embryonic ventricular myocytes. Circ. Res. 88, 536-542
36. Gonzalez-Sanchez, A., and Bader, D. (1990) In vitro analysis of cardiac progenitor cell differentiation. Dev. Biol. 139, 197-209
37. Jain, M. K., Kashiki, S., Hsieh, C. M., Layne, M. D., Yet, S. F., Sibinga, N. E., Chin, M. T., Feinberg, M. W., Woo, I., Maas, R. L., et al. (1998) Embryonic expression suggests an important role for CRP2/SmLIM in the developing cardiovascular system. Circ. Res. 83, 980-985
38. Li, L., Miano, J. M., Cserjesi, P., and Olson, E. N. (1996) SM22 alpha, a marker of adult smooth muscle, is expressed in multiple myogenic lineages during embryogenesis. Circ. Res. 78, 188 -195
39. Samaha, F. F., Ip, H. S., Morrisey, E. E., Seltzer, J., Tang, Z., Solway, J., and Parmacek, M. S. (1996) Developmental pattern of expression and genomic organization of the calponin-h1 gene. A contractile smooth muscle cell marker. J. Biol. Chem. 271, 395-403
40. Wu, S. M., Fujiwara, Y., Cibulsky, S. M., Clapham, D. E., Lien, C. L., Schultheiss, T. M., and Orkin, S. H. (2006) Developmental origin of a bipotential myocardial and smooth muscle cell precursor in the mammalian heart. Cell 127, 1137-1150
41. Gaudesius, G., Miragoli, M., Thomas, S. P., and Rohr, S. (2003) Coupling of cardiac electrical activity over extended distances by fibroblasts of cardiac origin. Circ. Res. 93, 421-428
42. Miragoli, M., Gaudesius, G., and Rohr, S. (2006) Electrotonic modulation of cardiac impulse conduction by myofibroblasts. Circ. Res. 98, 801-810
43. Kizana, E., Ginn, S. L., Smyth, C. M., Boyd, A., Thomas, S. P., Allen, D. G., Ross, D. L., and Alexander, I. E. (2006) Fibroblasts modulate cardiomyocyte excitability: implications for cardiac gene therapy. Gene Ther. 13, 1611-1615