Connexin43 silencing in myofibroblasts prevents arrhythmias in myocardial cultures: Role of maximal diastolic potential

Cardiovascular Research

Volumn 93, Issue 3, 2012, Pages 434-444

  Askar, Saïd F ^a   Bingen, Brian O ^a   Swildens, Jim ^a   Ypey, Dirk L ^a   Van Der Laarse, Arnoud ^a   Atsma, Douwe E ^a   Zeppenfeld, Katja ^a   Schalij, Martin J ^a   De Vries, Antoine A ^a   Pijnappels, Daniël A ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Arrhythmia; Connexins; Coupling; Mapping; Myofibroblasts

Indexed keywords

CONNEXIN 43; SHORT HAIRPIN RNA;

ACTION POTENTIAL; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; DEPOLARIZATION; DOWN REGULATION; GENE SILENCING; HEART ARRHYTHMIA; HEART MUSCLE CELL; HEART MUSCLE FIBER MEMBRANE POTENTIAL; HEART MUSCLE FIBROSIS; MYOFIBROBLAST; NERVE CONDUCTION; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REPOLARIZATION; TACHYCARDIA; VOLTAGE SENSITIVE DYE IMAGING;

ACTION POTENTIALS; ANIMALS; ANIMALS, NEWBORN; CONNEXIN 43; DIASTOLE; FIBROBLASTS; FIBROSIS; GENE THERAPY; HEART CONDUCTION SYSTEM; LENTIVIRUS; MYOCARDIUM; MYOCYTES, CARDIAC; PATCH-CLAMP TECHNIQUES; PRIMARY CELL CULTURE; RATS; RATS, WISTAR; RNA, SMALL INTERFERING; TACHYCARDIA;

EID: 84857591879     PISSN: 00086363     EISSN: 17553245     Source Type: Journal    
DOI: 10.1093/cvr/cvr351     Document Type: Article

References (24)

1. van der Burg AE, Bax JJ, Boersma E, Pauwels EK, van der Wall EE, Schalij MJ. Impact of viability, ischemia, scar tissue, and revascularization on outcome after aborted sudden death. Circulation 2003;108:1954-1959.
2. Arshad A, Mandava A, Kamath G, Musat D. Sudden cardiac death and the role of medical therapy. Prog Cardiovasc Dis 2008;50:420-438.
3. Manabe I, Shindo T, Nagai R. Gene expression in fibroblasts and fibrosis: involvement in cardiac hypertrophy. Circ Res 2002;91:1103-1113.
4. Sun Y, Kiani MF, Postlethwaite AE, Weber KT. Infarct scar as living tissue. Basic Res Cardiol 2002;97:343-347.
5. Miragoli M, Gaudesius G, Rohr S. Electrotonic modulation of cardiac impulse conduction by myofibroblasts. Circ Res 2006;98:801-810.
6. Miragoli M, Salvarani N, Rohr S. Myofibroblasts induce ectopic activity in cardiac tissue. Circ Res 2007;101:755-758.
7. Zlochiver S, Munoz V, Vikstrom KL, Taffet SM, Berenfeld O, Jalife J. Electrotonic myofibroblast-to-myocyte coupling increases propensity to reentrant arrhythmias in two-dimensional cardiac monolayers. Biophys J 2008;95:4469-4480.
8. Askar SF, Ramkisoensing AA, Schalij MJ, Bingen BO, Swildens J, van der Laarse A et al. Antiproliferative treatment of myofibroblasts prevents arrhythmias in vitro by limiting myofibroblast-induced depolarization. Cardiovasc Res 2011;90:295-304.
9. Pijnappels DA, Schalij MJ, van Tuyn J, Ypey DL, de Vries AA, van der Wall EE et al. Progressive increase in conduction velocity across human mesenchymal stem cells is mediated by enhanced electrical coupling. Cardiovasc Res 2006;72:282-291.
10. de Bakker JM, van Capelle FJ, Janse MJ, Tasseron S, Vermeulen JT, de Jonge N. et al. Slow conduction in the infarcted human heart. 'Zigzag' course of activation. Circulation 1993;88:915-926.
11. Spach MS, Dolber PC. Relating extracellular potentials and their derivatives to anisotropic propagation at a microscopic level in human cardiac muscle. Evidence for electrical uncoupling of side-to-side fiber connections with increasing age. Circ Res 1986;58:356-371.
12. Rook MB, Jongsma HJ, de Jonge B. Single channel currents of homo-and heterologous gap junctions between cardiac fibroblasts and myocytes. Pflugers Arch 1989;414:95-98.
13. Xie Y, Garfinkel A, Camelliti P, Kohl P, Weiss JN, Qu Z. Effects of fibroblast-myocyte coupling on cardiac conduction and vulnerability to reentry: a computational study. Heart Rhythm 2009;6:1641-1649.
14. McSpadden LC, Kirkton RD, Bursac N. Electrotonic loading of anisotropic cardiac monolayers by unexcitable cells depends on connexin type and expression level. Am J Physiol Cell Physiol 2009;297:C339-C351.
15. Pedrotty DM, Klinger RY, Badie N, Hinds S, Kardashian A, Bursac N. Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay. Am J Physiol Heart Circ Physiol 2008;295:H390-H400.
16. Vasquez C, Mohandas P, Louie KL, Benamer N, Bapat AC, Morley GE. Enhanced fibroblast-myocyte interactions in response to cardiac injury. Circ Res 2010;107:1011-1020.
17. Thompson SA, Copeland CR, Reich DH, Tung L. Mechanical coupling between myofibroblasts and cardiomyocytes slows electric conduction in fibrotic cell monolayers. Circulation 2011;123:2083-2093.
18. Hondeghem LM, Carlsson L, Duker G. Instability and triangulation of the action potential predict serious proarrhythmia, but action potential duration prolongation is antiarrhythmic. Circulation 2001;103:2004-2013.
19. Sato D, Xie LH, Sovari AA, Tran DX, Morita N, Xie F et al. Synchronization of chaotic early afterdepolarizations in the genesis of cardiac arrhythmias. Proc Natl Acad Sci U S A 2009;106:2983-2988.
20. Guo D, Zhao X, Wu Y, Liu T, Kowey PR, Yan GX. L-type calcium current reactivation contributes to arrhythmogenesis associated with action potential triangulation. J Cardiovasc Electrophysiol 2007;18:196-203.
21. Kleber AG, Rudy Y. Basic mechanisms of cardiac impulse propagation and associated arrhythmias. Physiol Rev 2004;84:431-488.
22. Kilborn MJ, Fedida D. A study of the developmental changes in outward currents of rat ventricular myocytes. J Physiol 1990;430:37-60.
23. Giles WR, Imaizumi Y. Comparison of potassium currents in rabbit atrial and ventricular cells. J Physiol 1988;405:123-145.
24. Rampaart LJ, Beekwilder JP, van Kempen GT, van den Berg RJ, Ypey DL. The local anesthetic butamben inhibits total and L-type barium currents in PC12 cells. Anesth Analg 2008;106:1778-1783.