A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells

Cancer Letters

Volumn 328, Issue 2, 2013, Pages 362-368

  Takahashi, Masayuki ^a   Elbarbary, Reyad A ^a   Nakashima, Aiko ^a   Abe, Mayumi ^a   Watanabe, Norihiro ^b   Narita, Miwako ^b   Takahashi, Masuhiro ^b   Tamura, Masato ^c   Yoshida, Tetsuo ^d   Nashimoto, Masayuki ^a  

^a NIIGATA UNIVERSITY OF PHARMACY AND APPLIED LIFE SCIENCES   (Japan)

^b NIIGATA UNIVERSITY   (Japan)

^c HOKKAIDO UNIVERSITY   (Japan)

^d KYOWA HAKKO KOGYO CO LTD   (Japan)

Author keywords

Bcl 2; Leukemia; Small guide RNA; TRNase ZL; TRUE gene silencing

Indexed keywords

GUIDE RNA; MESSENGER RNA; NAKED RNA; PROTEIN BCL 2; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; CONTROLLED STUDY; DRUG BINDING SITE; DRUG TARGETING; FEMALE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LEUKEMIA CELL; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SURVIVAL TIME; TUMOR XENOGRAFT;

ANIMALS; APOPTOSIS; CELL LINE, TUMOR; CELL PROLIFERATION; FEMALE; GENE SILENCING; GENES, BCL-2; HL-60 CELLS; HUMANS; LEUKEMIA; MICE; MICE, NUDE; RNA, GUIDE; RNA, MESSENGER; TRANSPLANTATION, HETEROLOGOUS;

MUS;

EID: 84870382135     PISSN: 03043835     EISSN: 18727980     Source Type: Journal    
DOI: 10.1016/j.canlet.2012.10.016     Document Type: Article

References (24)

1. Scherer L., Rossi J.J. Therapeutic potential of RNA-mediated control of gene expression: options and designs. RNA and the Regulation of Gene Expression: A Hidden Layer of Complexity 2008, 201-226. Caister Academic Press, Norfolk, UK. K.V. Morris (Ed.).
2. Nashimoto M. Conversion of mammalian tRNA 3' processing endoribonuclease to four-base-recognizing RNA cutters. Nucleic Acids Res. 1995, 23:3642-3647.
3. Nashimoto M. Specific cleavage of target RNAs from HIV-1 with 5' half tRNA by mammalian tRNA 3' processing endoribonuclease. RNA 1996, 2:523-524.
4. Nashimoto M. Distribution of both lengths and 5' terminal nucleotides of mammalian pre-tRNA 3' trailers reflects properties of 3' processing endoribonuclease. Nucleic Acids Res. 1997, 25:1148-1154.
5. Nashimoto M., Geary S., Tamura M., Kasper R. RNA heptamers that directs RNA cleavage by mammalian tRNA 3' processing endoribonuclease. Nucleic Acids Res. 1998, 26:2565-2572.
6. Nashimoto M. Anomalous RNA substrates for mammalian tRNA 3' processing endoribonuclease. FEBS Lett. 2000, 472:179-186.
7. Takaku H., Minagawa A., Masamichi T., Nashimoto M. A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoriobonuclease. Nucleic Acids Res. 2003, 31:2272-2278.
8. L through 5'-half-tRNA. PLoS ONE 2009, 4:e5908.
9. L can downregulate gene expression through miRNA. FEBS Lett. 2009, 583:3241-3246.
10. Tamura M., Nashimoto C., Miyake N., Daikuhara Y., Ochi K., Nashimoto M. Intracellular mRNA cleavage by 3' tRNase under the direction of 2'-O-methyl RNA heptamers. Nucleic Acids Res. 2003, 31:4354-4360.
11. Habu Y., Miyano-Kurosaki N., Kitano M., Endo Y., Yukita M., Ohira S., Takaku H., Nashimoto M., Takaku H. Inhibition of HIV-1 gene expression by retroviral vector-mediated small-guide RNAs that direct specific RNA cleavage by tRNase ZL. Nucleic Acids Res. 2005, 33:235-243.
12. L under the direction of small guide RNA. Gene Therapy 2007, 14:78-85.
13. Elbarbary R.A., Takaku H., Tamura M., Nashimoto M. Inhibition of vascular endothelial growth factor expression by TRUE gene silencing. Biochem. and Biophys. Res. Commun. 2009, 379:924-927.
14. Sano T., Takahashi M., Nozaki T., Takahashi Y., Tamura M., Nashimoto M. Expanding the utility of heptamer-type sgRNA for TRUE gene silencing. Biochem. and Biophys. Res. Commun. 2011, 416:427-432.
15. Takahashi M., Elbarbary R.A., Abe M., Sato M., Yoshida T., Yamada Y., Tamura M., Nashimoto M. Elimination of specific miRNAs by naked 14-nt sgRNAs. PLoS ONE 2012, 7:e38496.
16. Takaku H., Minagawa A., Takagi M., Nashimoto M. The N-terminal half-domain of the long form of tRNase Z is required for the RNase 65 activity. Nucleic Acids Res. 2004, 32:4429-4438.
17. Takaku H., Minagawa A., Takagi M., Nashimoto M. A novel four-base-recognizing RNA cutter that can remove the single 3' terminal nucleotides from RNA molecules. Nucleic Acids Res. 2004, 32:e91.
18. Shibata H.S., Takaku H., Takagi M., Nashimoto M. The T loop structure is dispensable for substrate recognition by tRNase ZL. J. Biol. Chem. 2005, 280:22326-22334.
19. Appasani K. RNA Interference Technology: from Basic Science to Drug Development 2005, Cambridge University Press, Cambridge, UK.
20. Sawyers C. Targeted cancer therapy. Nature 2004, 432:294-297.
21. Kelly P.N., Strasser A. The role of Bcl-2 and its pro-survival relatives in tumourigenesis and cancer therapy. Cell Death Differ. 2011, 18:1414-1424.
22. Loke S.L., Stein C.A., Zhang X.H., Mori K., Nakanishi M., Subasinghe C., Cohen J.S., Neckers L.M. Characterization of oligonucleotide transport into living cells. Proc. Natl. Acad. Sci. USA 1989, 86:3474-3478.
23. Stein C.A., Hansen J.B., Lai J., Wu S., Voskresenskiy A., Høg A., Worm J., Hedtjärn M., Souleimanian N., Miller P., Soifer H.S., Castanotto D., Benimetskaya L., Ørum H., Koch T. Efficient gene silencing by delivery of locked nucleic acid antisense oligonucleotides, unassisted by transfection reagents. Nucleic Acids Res. 2010, 38:e3.
24. Koller E., Vincent T.M., Chappell A., De S., Manoharan M., Bennett C.F. Mechanisms of single-stranded phosphorothioate modified antisense oligonucleotide accumulation in hepatocytes. Nucleic Acids Res. 2011, 39:4795-4807.