Ultra-structural study of insulin granules in pancreatic β-cells of db/db mouse by scanning transmission electron microscopy tomography

Protein and Cell

Volumn 3, Issue 7, 2012, Pages 521-525

  Xue, Yanhong ^a   Zhao, Wei ^a   Du, Wen ^a   Zhang, Xiang ^a   Ji, Gang ^a   Ying, Wang ^a   Xu, Tao ^a,b  

^a INSTITUTE OF BIOPHYSICS   (China)

^b HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

3D reconstruction; diabetes; insulin; secretory granule; stem; tomography

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BODY WEIGHT; CELL COUNT; CELL ULTRASTRUCTURE; CONTROLLED STUDY; ELECTRON TOMOGRAPHY; HYPOGLYCEMIA; IMAGE RECONSTRUCTION; INSULIN GRANULE; INSULIN METABOLISM; INSULIN RELEASE; INSULIN RESISTANCE; MALE; MOUSE; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; PANCREAS ISLET BETA CELL; PANCREAS ISLET CELL FUNCTION; PRIORITY JOURNAL; SCANNING TRANSMISSION ELECTRON MICROSCOPY; SECRETORY GRANULE; THREE DIMENSIONAL IMAGING;

ANIMALS; DIABETES MELLITUS, TYPE 2; ELECTRON MICROSCOPE TOMOGRAPHY; INSULIN; INSULIN-SECRETING CELLS; MALE; MICE; MICE, INBRED C57BL; MICE, OBESE; SECRETORY VESICLES;

EID: 84870313255     PISSN: 1674800X     EISSN: 16748018     Source Type: Journal    
DOI: 10.1007/s13238-012-2937-1     Document Type: Article

References (19)

1. Barg, S., Eliasson, L., Renstrom, E., and Rorsman, P. (2002). A subset of 50 secretory granules in close contact with L-type Ca2+ channels accounts for first-phase insulin secretion in mouse beta-cells. Diabetes 51Suppl 1, S74-82.
2. Boquist, L., Hellman, B., Lernmark, A., and Taljedal, I. B. (1974). Influence of the mutation "diabetes" on insulin release and islet morphology in mice of different genetic backgrounds. J Cell Biol 62, 77-89.
3. Dean, P. M. (1973). Ultrastructural morphometry of the pancreatic-cell. Diabetologia 9, 115-119.
4. Diani, A. R., Peterson, T., Sawada, G. A., Wyse, B. M., Gilchrist, B. J., Hearron, A. E., and Chang, A. Y. (1984). Ciglitazone, a new hypoglycaemic agent. 4. Effect on pancreatic islets of C57BL/6J-ob/ob and C57BL/KsJ-db/db mice. Diabetologia 27, 225-234.
5. Leiter, E. H., Coleman, D. L., and Eppig, J. J. (1979). Endocrine pancreatic cells of postnatal "diabetes" (db) mice in cell culture. In Vitro 15, 507-521.
6. Nagamatsu, S., Nakamichi, Y., Yamamura, C., Matsushima, S., Watanabe, T., Ozawa, S., Furukawa, H., and Ishida, H. (1999). Decreased expression of t-SNARE, syntaxin 1, and SNAP-25 in pancreatic beta-cells is involved in impaired insulin secretion from diabetic GK rat islets: restoration of decreased t-SNARE proteins improves impaired insulin secretion. Diabetes 48, 2367-2373.
7. Nakamura, M., Kitamura, H., Konishi, S., Nishimura, M., Ono, J., Ina, K., Shimada, T., and Takaki, R. (1995). The endocrine pancreas of spontaneously diabetic db/db mice: microangiopathy as revealed by transmission electron microscopy. Diabetes Res Clin Pract 30, 89-100.
8. Olofsson, C. S., Gopel, S. O., Barg, S., Galvanovskis, J., Ma, X., Salehi, A., Rorsman, P., and Eliasson, L. (2002). Fast insulin secretion reflects exocytosis of docked granules in mouse pancreatic B-cells. Pflugers Arch 444, 43-51.
9. Ostenson, C. G., Gaisano, H., Sheu, L., Tibell, A., and Bartfai, T. (2006). Impaired gene and protein expression of exocytotic soluble N-ethylmaleimide attachment protein receptor complex proteins in pancreatic islets of type 2 diabetic patients. Diabetes 55, 435-440.
10. Ostenson, C. G., Khan, A., Abdel-Halim, S. M., Guenifi, A., Suzuki, K., Goto, Y., and Efendic, S. (1993). Abnormal insulin secretion and glucose metabolism in pancreatic islets from the spontaneously diabetic GK rat. Diabetologia 36, 3-8.
11. Porter, A. E., Gass, M., Muller, K., Skepper, J. N., Midgley, P. A., and Welland, M. (2007). Direct imaging of single-walled carbon nanotubes in cells. Nat Nanotechnol 2, 713-717.
12. Porter, A. E., Muller, K., Skepper, J., Midgley, P., and Welland, M. (2006). Uptake of C60 by human monocyte macrophages, its localization and implications for toxicity: studied by high resolution electron microscopy and electron tomography. Acta Biomater 2, 409-419.
13. Portha, B., Giroix, M. H., Serradas, P., Welsh, N., Hellerstrom, C., Sener, A., and Malaisse, W. J. (1988). Insulin production and glucose metabolism in isolated pancreatic islets of rats with NIDDM. Diabetes 37, 1226-1233.
14. Rorsman, P., Eliasson, L., Renstrom, E., Gromada, J., Barg, S., and Gopel, S. (2000). The Cell Physiology of Biphasic Insulin Secretion. News Physiol Sci 15, 72-77.
15. Rorsman, P., and Renstrom, E. (2003). Insulin granule dynamics in pancreatic beta cells. Diabetologia 46, 1029-1045.
16. Sougrat, R., Bartesaghi, A., Lifson, J. D., Bennett, A. E., Bess, J. W., Zabransky, D. J., and Subramaniam, S. (2007). Electron tomography of the contact between T cells and SIV/HIV-1: implications for viral entry. PLoS Pathog 3, e63.
17. Suckale, J., and Solimena, M. (2008). Pancreas islets in metabolic signaling-focus on the beta-cell. Front Biosci 13, 7156-7171.
18. Suckale, J., and Solimena, M. (2010). The insulin secretory granule as a signaling hub. Trends Endocrinol Metab 21, 599-609.
19. Yakushevska, A. E., Lebbink, M. N., Geerts, W. J., Spek, L., van Donselaar, E. G., Jansen, K. A., Humbel, B. M., Post, J. A., Verkleij, A. J., and Koster, A. J. (2007). STEM tomography in cell biology. J Struct Biol 159, 381-391.