Isoform separation and binding site determination of mono-PEGylated lysozyme with pH gradient chromatography

Journal of Chromatography A

Volumn 1268, Issue , 2012, Pages 102-108

  Maiser, Benjamin ^a   Kröner, Frieder ^a   Dismer, Florian ^a   Brenner Weiß, Gerald ^b   Hubbuch, Jürgen ^a  

^a KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

^b KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

Author keywords

Isoform separation; PH gradient chromatography; Protein PEGylation

Indexed keywords

BIOPHARMACEUTICALS; COVALENT ATTACHMENT; ELUTION ORDER; ELUTION PH; ION EXCHANGE CHROMATOGRAPHY; ISO-ELECTRIC POINTS; ISOFORMS; MALDI-MS; N-TERMINALS; PEGYLATED PROTEINS; PEGYLATION; PH GRADIENTS; PROTEIN PEGYLATION; SALT GRADIENT; STRATEGY USE; TRYPTIC DIGESTION;

ALDEHYDES; AMINO ACIDS; CHROMATOGRAPHIC ANALYSIS; ENZYMES; IONIC STRENGTH; MOTION PICTURE EXPERTS GROUP STANDARDS; ORGANIC COMPOUNDS; PH; PROTEINS; SEPARATION;

CHROMATOGRAPHY;

ISOENZYME; LYSOZYME;

AMINO TERMINAL SEQUENCE; ANALYTIC METHOD; ARTICLE; BINDING SITE; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ISOLATION; ION EXCHANGE CHROMATOGRAPHY; ISOELECTRIC POINT; MASS SPECTROMETRY; PH GRADIENT CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN MODIFICATION; SEPARATION TECHNIQUE;

BINDING SITES; CHROMATOGRAPHY, ION EXCHANGE; ISOENZYMES; MURAMIDASE; POLYETHYLENE GLYCOLS; PROTON-MOTIVE FORCE; SOLUBILITY;

EID: 84869866228     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2012.10.047     Document Type: Article

References (30)

1. Abuchowski A., Vanes T., Palczuk N.C., Davis F.F. J. Biol. Chem. 1977, 252:3578.
2. Nodake Y., Yamasaki N. Biosci. Biotechnol. Biochem. 2000, 64:767.
3. Lee B.K., Kwon J.S., Kim H.J., Yamamoto S., Lee E.K. Bioconjug. Chem. 2007, 18:1728.
4. Rajan R.S., Li T., Aras M., Sloey C., Sutherland W., Arai H., Briddell R., Kinstler O., Lueras A.M.K., Zhang Y., et al. Protein Sci. 2006, 15:1063.
5. Veronese F.M., Pasut G. Drug Discov. Today 2005, 10:1451.
6. Harris J.M., Martin N.E., Modi M. Clin. Pharmacokinet. 2001, 40:539.
7. Gaberc-Porekar V., Zore I., Podobnik B., Menart V. Curr. Opin. Drug Discov. Dev. 2008, 11:242.
8. Kinstler O., Molineux G., Treuheit M., Ladd D., Gegg C. Adv. Drug Deliv. Rev. 2002, 54:477.
9. Kinstler O.B., Brems D.N., Lauren S.L., Paige A.G., Hamburger J.B., Treuheit M.J. Pharm. Res. 1996, 13:996.
10. Abe M., Akbarzaderaleh P., Hamachi M., Yoshimoto N., Yamamoto S. Biotechnol. J. 2010, 5:477.
11. PEGylated Protein Drugs: Basic Science and Clinical Applications 2009, 205. Birkhäuser Verlag. G. Pasut, F.M. Veronese (Eds.).
12. Veronese F.M., Pasut G. Drug Discov. Today 2008, 5:e57.
13. Monkarsh S.P., Ma Y.M., Aglione A., Bailon P., Ciolek D., DeBarbieri B., Graves M.C., Hollfelder K., Michel H., Palleroni A., et al. Anal. Biochem. 1997, 247:434.
14. Hansen S.K., Maiser B., Hubbuch J. J. Chromatogr. A 2012, 1257:41.
15. Moosmann A., Blath J., Lindner R., Müller E., Böttinger H. Bioconjug. Chem. 2011, 22:1545.
16. Foser S., Schacher A., Weyer K.A., Brugger D., Dietel E., Marti S., Schreitmüller T. Protein Expr. Purif. 2003, 30:78.
17. Fee C.J., Van Alstine J.A. Chem. Eng. Sci. 2006, 61:924.
18. Yamamoto S., Fujii S., Yoshimoto N., Akbarzadehlaleh P. J. Biotechnol. 2007, 132:196.
19. PEGylated Protein Drugs: Basic Science and Clinical Applications 2009, 113. Birkhäuser Verlag. C.J. Fee, F.M. Veronese (Eds.).
20. Ng P.K., He J., Snyder M. J. Chromatogr. A 2009, 1216:1372.
21. Lee H., Park T.G. J. Pharm. Sci. 2003, 92:97.
22. Kinstler O.B., Brems D.N., Lauren S.L., Paige A.G., Hamburger J.B., Treuheit M.J. Pharm. Res. 1996, 13:996.
23. Michael J.R., Harris J.M. J. Pharm. Sci. 1998, 87:1440.
24. Bashford D., Gerwert K. J. Mol. Biol. 1992, 224:473.
25. Bashford D. An Object-oriented Programming Suite for Electrostatic Effects in Biological Molecules. An Experience Report on the MEAD Project 1997, Springer, pp. 233-240.
26. Fee C.J., Van Alstine J.M. Bioconjug. Chem. 2004, 15:1304.
27. Moosmann A., Christel J., Böttinger H., Müller E. J. Chromatogr. A 2010, 1217:209.
28. Ahamed T., Nfor B.K., Verhaert P., van Dedem G., van der Wielen L., Eppink M., van de Sandt E., Ottens M. J. Chromatogr. A 2007, 1164:181.
29. Miteva M.A., Tuffery P., Villoutreix B.O. Nucleic Acids Res. 2005, 33:W372.
30. Daly S.M., Przybycien T.M., Tilton R.D. Langmuir 2005, 21:1328.