Human recombinant beta-secretase immobilized enzyme reactor for fast hits' selection and characterization from a virtual screening library

Journal of Pharmaceutical and Biomedical Analysis

Volumn 73, Issue , 2013, Pages 131-134

  De Simone, Angela ^a   Mancini, Francesca ^b   Cosconati, Sandro ^c   Marinelli, Luciana ^d   La Pietra, Valeria ^d   Novellino, Ettore ^d   Andrisano, Vincenza ^b  

^a ISTITUTO ITALIANO DI TECNOLOGIA   (Italy)

^b UNIVERSITY OF BOLOGNA   (Italy)

^c SECOND UNIVERSITY OF NAPLES   (Italy)

^d UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

HrBACE1 IMER; Inhibition mechanism; Inhibitor hits screening; LC fluorescent detection; Online enzyme activity

Indexed keywords

AMYLOID PRECURSOR PROTEIN; INDANONE DERIVATIVE; RECOMBINANT BETA SECRETASE 1; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ARTICLE; BIOREACTOR; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; ENZYME INHIBITION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR LIBRARY; MUTATION; PRIORITY JOURNAL; PROTEIN CLEAVAGE; SENSITIVITY ANALYSIS;

AMYLOID PRECURSOR PROTEIN SECRETASES; ASPARTIC ACID ENDOPEPTIDASES; BIOREACTORS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME INHIBITORS; ENZYMES, IMMOBILIZED; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; SENSITIVITY AND SPECIFICITY; SMALL MOLECULE LIBRARIES; SUBSTRATE SPECIFICITY;

EID: 84869500941     PISSN: 07317085     EISSN: 1873264X     Source Type: Journal    
DOI: 10.1016/j.jpba.2012.03.006     Document Type: Article

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