Effect of freezing-thawing process and quercetin on human sperm survival and DNA integrity

Cryobiology

Volumn 65, Issue 3, 2012, Pages 326-331

  Zribi, Nassira ^a   Chakroun, Nozha Feki ^a   Ben Abdallah, Fatma ^a   Elleuch, Henda ^b   Sellami, Afifa ^a   Gargouri, Jalel ^b   Rebai, Tarek ^a   Fakhfakh, Faiza ^a   Keskes, Leila Ammar ^a  

^a UNIVERSITY OF SFAX   (Tunisia)

^b Sfax Regional Center of Blood Transfusion of El Ain Road ^*  (Tunisia)

Author keywords

Caspase 3; DNA integrity; Quercetin; Sperm cryopreservation

Indexed keywords

CASPASE 3; QUERCETIN;

APOPTOSIS; ARTICLE; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; CRYOPRESERVATION; DNA DETERMINATION; DNA FRAGMENTATION; ENZYME ACTIVATION; FREEZE THAWING; HUMAN; HUMAN CELL; MALE; MALE INFERTILITY; NICK END LABELING; OXIDATIVE STRESS; PRIORITY JOURNAL; SEMEN ANALYSIS; SPERM PRESERVATION; SPERMATOGENESIS; SPERMATOZOON MOTILITY;

ANTIOXIDANTS; CASPASE 3; CELL SURVIVAL; CRYOPRESERVATION; DNA; DNA FRAGMENTATION; HUMANS; IN SITU NICK-END LABELING; MALE; OXIDATIVE STRESS; QUERCETIN; SEMEN PRESERVATION; SPERMATOZOA;

EID: 84869165847     PISSN: 00112240     EISSN: 10902392     Source Type: Journal    
DOI: 10.1016/j.cryobiol.2012.09.003     Document Type: Article

References (33)

1. Aherne S.A., O'Brien N.M. Mechanism of protection by the flavonoids, quercetin and rutin, against tert-butylhydroperoxide- and menadione-induced DNA single strand breaksin Caco-2 cells. Free. Radic. Biol. Med. 2000, 29:507-514.
2. Ben Abdallah F., Zribi N., Ammar-Keskes L. Antioxidative potential of Quercetin against hydrogen peroxide induced oxidative stress in spermatozoa in vitro. Andrologia 2011, 43:261-265.
3. Branco C.S., Garcez M.E., Pasqualotto F.F., Erdtman B., Salvador M. Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen. Cryobiology 2010, 60:235-237.
4. Brum A.M., Sabeur K., Ball B.A. Apoptotic-like changes in equine spermatozoa separated by density-gradient centrifugation or after cryopreservation. Theriogenology 2008, 69:1041-1055.
5. Bucak M.N., Tuncer P.B., Sari{dotless}özkan S., Başpi{dotless}nar N., Taşpi{dotless}nar M., Coyan K., Bilgili A., Akali{dotless}n P.P., Büyükleblebici S., Aydos S., Ilgaz S., Sunguroĝlu A., Oztuna D. Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: antioxidants protect DNA integrity against cryodamage. Cryobiology 2010, 61:248-253.
6. De Paula T.S., Bertolla R.P., Spaine D.M., Cunha M.A., Schor N., Cedenho A.P. Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia. Fertil. Steril. 2006, 86:597-600.
7. Di Santo M., Tarozzi N., Nadalini M., Borini A. Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART. Adv. Urol. 2012, Epub 2011.
8. Garcez M.E., dos Santos Branco C., Lara L.V., Pasqualotto F.F., Salvador M. Effects of resveratrol supplementation on cryopreservation medium of human semen. Fertil. Steril. 2010, 94:2118-2121.
9. Hammadeh M.E., Askari A.S., Georg T., Rosenbaum P., Schmidt W. Effect of freeze-thawing procedure on chromatin stability, morphological alteration and membrane integrity of human spermatozoa in fertile and subfertile men. Int. J. Androl. 1999, 22:155-162.
10. Isachenko V., Isachenko E., Katkov I.I., Montag M., Dessole S., Nawroth F., Van Der Ven H. Cryoprotectant-free cryopreservation of human spermatozoa by vitrification and freezing in vapour: effect on motility, DNA integrity, and fertilization ability. Biol. Reprod. 2004, 71:1167-1173.
11. Li Z.L., Lin Q.L., Liu R.J., Xie W.Y., Xiao W.F. Reducing oxidative DNA damage by adding antioxidants in human semen samples undergoing cryopreservation procedure. Zhonghua Yi Xue Za Zhi 2007, 87:3174-3177.
12. Martin G., Sabido O., Durand P., Levy R. Cryopreservation induces an apoptosis-like mechanism in bull sperm. Biol. Reprod. 2004, 71:28-37.
13. Mazzilli F., Rossi T., Sabatini L., Pulcinelli F.M., Rapone S., Dondero F., Gazzaniga P.P. Human sperm cryopreservation and reactive oxygen species (ROS) production. Acta Eur. Fertil. 1995, 26:145-148.
14. Meamar M., Zribi N., Cambi M., Tamburrino L., Marchiani S., Filimberti E., Fino M.G., Biggeri A., Menezo Y., Forti G., Baldi E., Muratori M. Sperm DNA fragmentation induced by cryopreservation: new insights and effect of a natural extract from Opuntia ficus-indica. Fertil. Steril. 2012, 98:326-333.
15. Muldrew K., Mc Gann L.E. Mechanisms of intracellular ice formation. Biophys. J. 1990, 57:525-532.
16. Paasch U., Grunewald S., Agarwal A., Glandera H.J. Activation pattern of caspases in human spermatozoa. Fertil. Steril. 2004, 81:802-809.
17. Paasch U., Sharma R.K., Gupta A.K., Grunewald S., Mascha E.J., Thomas A.J., Glander H.J., Agarwal A. Cryopreservation and thawing is associated with varying extend of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Biol. Reprod. 2004, 71:1828-1837.
18. Peris S.I., Morrier A., Dufour M., Bailey J.L. Cryopreservation of ram semen facilitates sperm DNA damage: relationship between sperm andrological parameters and the sperm chromatin structure assay. J. Androl. 2004, 25:224-233.
19. Peris S.I., Bilodeau J.F., Dufour M., Bailey J.L. Impact of cryopreservation and reactive oxygen species on DNA integrity, lipid peroxidation, and functional parameters in ram sperm. Mol. Reprod. Dev. 2007, 74:878-892.
20. Said T.M., Gaglani A., Agarwal A. Implication of apoptosis in sperm cryoinjury. Reprod. Biomed. Online 2010, 21:456-462.
21. Sariözkan S., Bucak M.N., Tuncer P.B., Ulutaş P.A., Bilgen A. The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation. Cryobiology 2009, 58:134-138.
22. Silva E.C., Cajueiro J.F., Silva S.V., Soares P.C., Guerra M.M. Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm. Theriogenology 2012, 77:1722-1726.
23. Sinclair S. Male infertility: nutritional and environmental considerations. Altern. Med. Rev. 2000, 5:28-38.
24. Solary E., Eymin B., Droin N., Haugg M. Proteases, proteolysis, and apoptosis. Cell Biol. Toxicol. 1998, 14:121-132.
25. Tatone C., Di Emidio G., Vento M., Ciriminna R., Artini P.G. Cryopreservation and oxidative stress in reproductive cells. Gynecol. Endocrinol. 2010, 26:563-567.
26. Taylor K., Roberts P., Saunders K., Burton P. Effect of antioxidant supplementation of cryopreservation medium on post-thaw integrity of human spermatozoa. Reprod. Biomed. Online 2009, 18:184-189.
27. Thomson L.K., Fleming S.D., Aitken R.J., De Iuliis G.N., Zieschang J.A., Clark A.M. Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Hum. Reprod. 2009, 24:2061-2070.
28. Thuwanut P., Chatdarong K., Johannisson A., Bergqvist A.S., Söderquist L., Axnér E. Cryopreservation of epididymal cat spermatozoa: effects of in vitro antioxidative enzymes supplementation and lipid peroxidation induction. Theriogenology 2010, 73:1076-1087.
29. Tremellen K. Oxidative stress and male infertility - a clinical perspective. Hum. Reprod. Update 2008, 14:243-258.
30. Wang A.W., Zhang H., Ikemoto I., Anderson D.J., Loughlin K.R. Reactive oxygen species generation by seminal cells during cryopreservation. Urology 1997, 49:921-925.
31. Watson P.F. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 2000, 60-61:481-492.
32. World Health Organization (WHO), Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, fourth ed., Cambridge, Cambridge University Press, 1999.
33. Zribi N., Feki Chakroun N., El Euch H., Gargouri J., Bahloul A., Ammar Keskes L. Effects of cryopreservation on human sperm deoxyribonucleic acid integrity. Fertil. Steril. 2010, 93:159-166.