Exploiting the intron-splicing mechanism of insect cells to produce viral vectors harboring toxic genes for suicide gene therapy

Molecular Therapy Nucleic Acids

Volumn 1, Issue 11, 2012, Pages e57-

  Chen, Haifeng ^a  

^a Haifeng Chen RandD Virovek Inc   (United States)

Author keywords

Adeno associated virus (AAV); Baculovirus; Cancer; Gene therapy; Toxin

Indexed keywords

BACULOVIRUS VECTOR; BARNASE; CYTOTOXIC FACTOR; DIPHTHERIA TOXIN FRAGMENT A; PARVOVIRUS VECTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL KILLING; INSECT CELL; INTRON; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RNA SPLICING; SUICIDE GENE THERAPY;

EID: 84869152168     PISSN: None     EISSN: 21622531     Source Type: Journal    
DOI: 10.1038/mtna.2012.48     Document Type: Article

References (22)

1. Rodriguez, R and Simons, JW (1999). Urologic applications of gene therapy. Urology 54: 401-"406.
2. Yamaizumi, M, Mekada, E, Uchida, T and Okada, Y (1978). One molecule of diphtheria toxin fragment A introduced into a cell can kill the cell. Cell 15: 245-"250.
3. Denning, C and Pitts, JD (1997). Bystander effects of different enzyme-prodrug systems for cancer gene therapy depend on different pathways for intercellular transfer of toxic metabolites, a factor that will govern clinical choice of appropriate regimes. Hum Gene Ther 8: 1825-"1835.
4. Maxwell, IH, Maxwell, F and Glode, LM (1986). Regulated expression of a diphtheria toxin A-chain gene transfected into human cells: Possible strategy for inducing cancer cell suicide. Cancer Res 46: 4660-"4664.
5. Maxwell, F, Maxwell, IH and Glode, LM (1987). Cloning, sequence determination, and expression in transfected cells of the coding sequence for the tox 176 attenuated diphtheria toxin A chain. Mol Cell Biol 7: 1576-"1579.
6. Robinson, DF and Maxwell, IH (1995). Suppression of single and double nonsense mutations introduced into the diphtheria toxin A-chain gene: A potential binary system for toxin gene therapy. Hum Gene Ther 6: 137-"143.
7. Paulus, W, Baur, I, Oberer, DM, Breakefield, XO and Reeves, SA (1997). Regulated expression of the diphtheria toxin A gene in human glioma cells using prokaryotic transcriptional control elements. J Neurosurg 87: 89-"95.
8. Keyvani, K, Baur, I and Paulus, W (1999). Tetracycline-controlled expression but not toxicity of an attenuated diphtheria toxin mutant. Life Sci 64: 1719-"1724.
9. Wang, CY, Li, F, Yang, Y, Guo, HY, Wu, CX and Wang, S (2006). Recombinant baculovirus containing the diphtheria toxin A gene for malignant glioma therapy. Cancer Res 66: 5798-"5806.
10. Li, Y, McCadden, J, Ferrer, F, Kruszewski, M, Carducci, M, Simons, J et al. (2002). Prostate-specific expression of the diphtheria toxin A chain (DT-A): Studies of inducibility and specificity of expression of prostate-specific antigen promoter-driven DT-A adenoviralmediated gene transfer. Cancer Res 62: 2576-"2582.
11. Kohlschütter, J, Michelfelder, S and Trepel, M (2010). Novel cytotoxic vectors based on adeno-associated virus. Toxins (Basel) 2: 2754-"2768.
12. Weaver, RF (2002). Molecular Biology. McGraw-Hill: Boston.
13. Urabe, M, Ding, C and Kotin, RM (2002). Insect cells as a factory to produce adeno-associated virus type 2 vectors. Hum Gene Ther 13: 1935-"1943.
14. Jeang, KT, Holmgren-Konig, M and Khoury, G (1987). A baculovirus vector can express intron-containing genes. J Virol 61: 1761-"1764.
15. He, F, Ho, Y, Yu, L and Kwang, J (2008). WSSV ie1 promoter is more efficient than CMV promoter to express H5 hemagglutinin from influenza virus in baculovirus as a chicken vaccine. BMC Microbiol 8: 238.
16. High, KA and Aubourg, P (2011). rAAV human trial experience. Methods Mol Biol 807: 429-"457.
17. Zhu, ZB, Makhija, SK, Lu, B, Wang, M, Kaliberova, L, Liu, B et al. (2004). Transcriptional targeting of tumors with a novel tumor-specific survivin promoter. Cancer Gene Ther 11: 256-"262.
18. Zhu, ZB, Makhija, SK, Lu, B, Wang, M, Kaliberova, L, Liu, B et al. (2004). Transcriptional targeting of adenoviral vector through the CXCR4 tumor-specific promoter. Gene Ther 11: 645-"648.
19. Hallenbeck, PL, Chang, YN, Hay, C, Golightly, D, Stewart, D, Lin, J et al. (1999). A novel tumor-specific replication-restricted adenoviral vector for gene therapy of hepatocellular carcinoma. Hum Gene Ther 10: 1721-"1733.
20. Gu, J, Andreeff, M, Roth, JA and Fang, B (2002). hTERT promoter induces tumor-specific Bax gene expression and cell killing in syngenic mouse tumor model and prevents systemic toxicity. Gene Ther 9: 30-"37.
21. Chen, H (2008). Intron splicing-mediated expression of AAV Rep and Cap genes and production of AAV vectors in insect cells. Mol Ther 16: 924-"930.
22. Merten, O-W and Al-Rubeai, M. Viral Vectors for Gene Therapy: Methods and Protocols. Humana Press: New York.