Mitigation of isolation-associated adipocyte interleukin-6 secretion following rapid dissociation of adipose tissue

Journal of Lipid Research

Volumn 53, Issue 12, 2012, Pages 2797-2805

  Thompson, Airlia C S ^a   Nuñez, Martha ^a   Davidson, Ryan ^a   Horm, Teresa ^a   Schnittker, Karina ^a   Hart, Madeline V ^a   Suarez, Allen M ^a   Tsao, Tsu Shuen ^a  

^a The University of Arizona   (United States)

Author keywords

Collagenase and insulin sensitivity; Digestion; Inflammation; Obesity

Indexed keywords

COLLAGENASE; INSULIN; INTERLEUKIN 6; MITOGEN ACTIVATED PROTEIN KINASE P38;

ADIPOCYTE; ADIPOSE TISSUE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL ISOLATION; CELL LYSATE; DISSOCIATION; ENZYME LINKED IMMUNOSORBENT ASSAY; INFLAMMATION; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RAT; WESTERN BLOTTING;

ADIPOCYTES; ADIPOSE TISSUE; ANIMALS; ARTIFACTS; CELL SEPARATION; INSULIN; INTERLEUKIN-6; MALE; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHORYLATION; RATS; RATS, SPRAGUE-DAWLEY;

EID: 84869070925     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D031286     Document Type: Article

References (33)

1. Kahn, S. E., R. L. Hull, and K. M. Utzschneider. 2006. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature. 444: 840-846.
2. Allende-Vigo, M. Z. 2010. Pathophysiologic mechanisms linking adipose tissue and cardiometabolic risk. Endocr. Pract. 16: 692-698.
3. Sun, K., C. M. Kusminski, and P. E. Scherer. 2011. Adipose tissue remodeling and obesity. J. Clin. Invest. 121: 2094-2101.
4. Anderson, E. K., D. A. Gutierrez, and A. H. Hasty. 2010. Adipose tissue recruitment of leukocytes. Curr. Opin. Lipidol. 21: 172-177.
5. Rodbell, M. 1964. Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J. Biol. Chem. 239: 375-380.
6. Foley, J. E., R. Foley, and J. Gliemann. 1980. Rate-limiting steps of 2-deoxyglucose uptake in rat adipocytes. Biochim. Biophys. Acta. 599: 689-698.
7. Gliemann, J., W. D. Rees, and J. A. Foley. 1984. The fate of labelled glucose molecules in the rat adipocyte. Dependence on glucose concentration. Biochim. Biophys. Acta. 804: 68-76.
8. Honnor, R. C., G. S. Dhillon, and C. Londos. 1985. cAMP-dependent protein kinase and lipolysis in rat adipocytes. II. Definition of steady-state relationship with lipolytic and antilipolytic modulators. J. Biol. Chem. 260: 15130-15138.
9. Joost, H. G., T. M. Weber, and S. W. Cushman. 1988. Qualitative and quantitative comparison of glucose transport activity and glucose transporter concentration in plasma membranes from basal and insulin-stimulated rat adipose cells. Biochem. J. 249: 155-161.
10. Malide, D., G. Ramm, S. W. Cushman, and J. W. Slot. 2000. Immunoelectron microscopic evidence that GLUT4 translocation explains the stimulation of glucose transport in isolated rat white adipose cells. J. Cell Sci. 113: 4203-4210.
11. Phillips, S. A., J. Kung, T. P. Ciaraldi, C. Choe, L. Christiansen, S. Mudaliar, and R. R. Henry. 2009. Selective regulation of cellular and secreted multimeric adiponectin by antidiabetic therapies in humans. Am. J. Physiol. Endocrinol. Metab. 297: E767-E773.
12. Qiao, L., B. Kinney, J. Schaack, and J. Shao. 2011. Adiponectin inhibits lipolysis in mouse adipocytes. Diabetes. 60: 1519-1527.
13. Fantuzzi, G. 2005. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 115: 911-919.
14. Ruan, H., M. J. Zarnowski, S. W. Cushman, and H. F. Lodish. 2003. Standard isolation of primary adipose cells from mouse epididymal fat pads induces inflammatory mediators and down-regulates adipocyte genes. J. Biol. Chem. 278: 47585-47593.
15. Ruan, H., N. Hacohen, T. R. Golub, L. Van Parijs, and H. F. Lodish. 2002. Tumor necrosis factor-alpha suppresses adipocyte-specific genes and activates expression of preadipocyte genes in 3T3-L1 adipocytes: nuclear factor-kappaB activation by TNF-alpha is obligatory. Diabetes. 51: 1319-1336.
16. Rotter, V., I. Nagaev, and U. Smith. 2003. Interleukin-6 (IL-6) induces insulin resistance in 3T3-L1 adipocytes and is, like IL-8 and tumor necrosis factor-alpha, overexpressed in human fat cells from insulin-resistant subjects. J. Biol. Chem. 278: 45777-45784.
17. Uno, T., J. He, I. Usui, Y. Kanatani, A. Bukhari, S. Fujisaka, Y. Yamazaki, H. Suzuki, M. Iwata, M. Ishiki, et al. 2008. Long-term interleukin-1alpha treatment inhibits insulin signaling via IL-6 production and SOCS3 expression in 3T3-L1 adipocytes. Horm. Metab. Res. 40: 8-12.
18. Serrano-Marco, L., R. Rodríguez-Calvo, I. El Kochairi, X. Palomer, L. Michalik, W. Wahli, and M. Vázquez-Carrera. 2011. Activation of peroxisome proliferator-activated receptor-β/-δ (PPAR-β/- δ) ameliorates insulin signaling and reduces SOCS3 levels by inhibiting STAT3 in interleukin-6-stimulated adipocytes. Diabetes. 60: 1990-1999.
19. Xie, L., M. T. Ortega, S. Mora, and S. K. Chapes. 2010. Interactive changes between macrophages and adipocytes. Clin. Vaccine Immunol. 17: 651-659.
20. Bastard, J-P., M. Maachi, J. T. Van Nhieu, C. Jardel, E. Bruckert, A. Grimaldi, J-J. Robert, J. Capeau, and B. Hainque. 2002. Adipose tissue IL-6 content correlates with resistance to insulin activation of glucose uptake both in vivo and in vitro. J. Clin. Endocrinol. Metab. 87: 2084-2089.
21. Zampetaki, A., Z. Zhang, Y. Hu, and Q. Xu. 2005. Biomechanical stress induces IL-6 expression in smooth muscle cells via Ras/Rac1-p38 MAPK-NF-kappaB signaling pathways. Am. J. Physiol. Heart Circ. Physiol. 288: H2946-H2954.
22. Jager, J., T. Grémeaux, M. Cormont, Y. Le Marchand-Brustel, and J-F. Tanti. 2007. Interleukin-1beta-induced insulin resistance in adipocytes through down-regulation of insulin receptor substrate-1 expression. Endocrinology. 148: 241-251.
23. Lagathu, C., L. Yvan-Charvet, J-P. Bastard, M. Maachi, A. Quignard-Boulangé, J. Capeau, and M. Caron. 2006. Long-term treatment with interleukin-1beta induces insulin resistance in murine and human adipocytes. Diabetologia. 49: 2162-2173.
24. Ruan, H., P. D. G. Miles, C. M. Ladd, K. Ross, T. R. Golub, J. M. Olefsky, and H. F. Lodish. 2002. Profiling gene transcription in vivo reveals adipose tissue as an immediate target of tumor necrosis factor-alpha: implications for insulin resistance. Diabetes. 51: 3176-3188.
25. Lagathu, C., J-P. Bastard, M. Auclair, M. Maachi, J. Capeau, and M. Caron. 2003. Chronic interleukin-6 (IL-6) treatment increased IL-6 secretion and induced insulin resistance in adipocyte: prevention by rosiglitazone. Biochem. Biophys. Res. Commun. 311: 372-379.
26. Fain, J. N., A. K. Madan, M. L. Hiler, P. Cheema, and S. W. Bahouth. 2004. Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans. Endocrinology. 145: 2273-2282.
27. Wang, B., I. S. Wood, and P. Trayhurn. 2007. Dysregulation of the expression and secretion of inflammation-related adipokines by hypoxia in human adipocytes. Pflugers Arch. 455: 479-492.
28. Kono, T., K. Suzuki, L. E. Dansey, F. W. Robinson, and T. L. Blevins. 1981. Energy-dependent and protein synthesis-independent recycling of the insulin-sensitive glucose transport mechanism in fat cells. J. Biol. Chem. 256: 6400-6407.
29. Weber, T. M., H. G. Joost, I. A. Simpson, and S. W. Cushman. 1988. Methods for assessment of glucose transport activity and the number of gluscose transporters in isolated rat adipose cells and membrane fractions. In Insulin Receptors: Clinical assessment, Biological Responses, and Comparison to the IGF-I Receptor. C. R. Kahn and L. C. Harrison, editors. A. R. Liss, NY. 171-187.
30. Wang, Y., J. Han, Y. Zang, and W. Guo. 2002. Effects of vanadate on leptin production from isolated rat adipocytes. Biol. Trace Elem. Res. 85: 171-182.
31. Blüher, M., M-E. Patti, S. Gesta, B. B. Kahn, and C. R. Kahn. 2004. Intrinsic heterogeneity in adipose tissue of fat-specific insulin receptor knock-out mice is associated with differences in patterns of gene expression. J. Biol. Chem. 279: 31891-31901.
32. Skurk, T., C. Alberti-Huber, C. Herder, and H. Hauner. 2007. Relationship between adipocyte size and adipokine expression and secretion. J. Clin. Endocrinol. Metab. 92: 1023-1033.
33. Carswell, K. A., M-J. Lee, and S. K. Fried. 2012. Culture of isolated human adipocytes and isolated adipose tissue. Methods Mol. Biol. 806: 203-214.