Culture of isolated human adipocytes and Isolated adipose tissue

Methods in Molecular Biology

Volumn 806, Issue , 2012, Pages 203-214

  Carswell, Kirstin A ^a   Lee, Mi Jeong ^b   Fried, Susan K ^b  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Adipocytes; Adipose tissue; Cell culture techniques

Indexed keywords

ADIPOCYTE; ADIPOSE TISSUE; ARTICLE; CELL SEPARATION; CYTOLOGY; HUMAN; METABOLISM; METHODOLOGY; PRIMARY CELL CULTURE; TISSUE CULTURE TECHNIQUE;

ADIPOCYTES; ADIPOSE TISSUE; CELL SEPARATION; HUMANS; PRIMARY CELL CULTURE; TISSUE CULTURE TECHNIQUES;

EID: 82355165006     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-367-7_14     Document Type: Article

References (48)

1. Arner P (1999) 8. The role of adipose tissue in lipoprotein metabolism. Atherosclerosis 146 Suppl. 1: S11-S12. (Pubitemid 29406129)
2. Trayhurn P, Wood IS (2004) Adipokines: inflammation and the pleiotropic role of white adipose tissue. Br J Nutr 92:347-55. (Pubitemid 40277057)
3. Coppack SW (2001) Pro-inflammatory cytokines and adipose tissue. Proc Nutr Soc 60: 349-56. (Pubitemid 32772450)
4. Klaus S (2004) Adipose tissue as a regulator of energy balance. Curr Drug Targets 5:241-50. (Pubitemid 38372310)
5. Rajala MW, Scherer PE (2003) Minireview: the adipocyte-at the crossroads of energy homeostasis, inflammation, and atherosclerosis. Endocrinology 144:3765-73. (Pubitemid 37088727)
6. Havel PJ (2004) Section IV: Lipid Modulators of islet function. Update of Adipocyte hormones. Regulation of energy balance and carbohydrate/ lipid metabolism. Diabetes 53 (Suppl. 1): S143-S151. (Pubitemid 38168707)
7. Trayhurn P (2007) Adipocyte biology. Obesity reviews 8 (Suppl.1): 41-44.
8. Zuk PA, Zhu M, Mizuno H et al. (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7: 211-228. (Pubitemid 32295155)
9. Guilak F, Lott KE, Awad HA et al. (2006) Clonal analysis of the differentiation potential of human adipose-derived adult stem cells. J Cell Physiol 206: 229-237. (Pubitemid 41740736)
10. Sgodda M, Aurich H, Kleist S, et al (2007) Hepatocyte differentiation of mesenchymal stem cells from rat peritoneal adipose tissue in vitro and in vivo. Exp Cell Res 313: 2875-2886. (Pubitemid 47135604)
11. Aurich H, Sgodda M, Kaltwasser P, et al (2009) Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo. Gut 58: 570-581.
12. Ruiz JC, Ludlow JW, Sherwood S, et al (2010) Differentiated human adipose-derived stem cells exhibit hepatogenic capability in vitro and in vivo. J Cell Phys 225: 429-436.
13. Zuk PA, Zhu M, Ashjian P, et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13: 4279-4295. (Pubitemid 35471185)
14. Weisberg SP, McCann D, Desai M, et al (2003) Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest 112: 1796-1808. (Pubitemid 38063703)
15. Fried SK, Moustaid-Moussa N (2001) Culture of adipose tissue and isolated adipocytes. In: Alihaud G (ed) Methods in Molecular Biology: Adipose Tissue Protocols, Volume 155. Humana Press.
16. Smith U, Jacobsson B (1973) Studies of human adipose tissue in culture. II. Effects of insulin and of medium glucose on lipolysis and cell size. Anat Record 176: 181-183.
17. Smith U (1971) Morphologic studies of human subcutaneous adipose tissue in vitro. Anat Rec 169: 97-104.
18. Robertson JP, Faulkner A, Vernon G (1982) Regulation of glycolysis and fatty acid synthesis from glucose in sheep adipose tissue. Biochem J 206: 577-586. (Pubitemid 13199530)
19. Tan BK, Chen J, Lahnert H, et al (2007) Raised serum, adipocyte, and adipose tissue retinolbinding protein overweight women with polycystic ovary syndrome: effects of gonadal adrenal steroids. J Clin Endocrinol Metab 92: 2764-2772. (Pubitemid 47037388)
20. Gerrits PM, Olson AL, Pessin JE (1993) Regulation of the GLUT4/muscle-fat glucose transporter mRNA in adipose tissue of insulindeficient diabetic rats. J Biol Chem 268: 640-644. (Pubitemid 23021370)
21. Appel B, Fried SK (1992) Effects of insulin and dexamethasone of lipoprotein lipase in human adipose tissue. Am J Physiol 262: E695-E699.
22. Wang Y, Fried SH, Petersen RN, et al (1999) Somatotropin regulates adipose tissue metabolism in neonatal swine. J Nutr 129: 139-145. (Pubitemid 29044772)
23. Fried SK, Russel CD, Grauso NL, et al (1993) Lipolprotein lipase regulation by insulin and glucocorticoid in subcutaneous and omental adipose tissues of obese women and men. J Clin Invest 92: 2191-2198. (Pubitemid 23333523)
24. Despres JP, Moorjani S, Lupien PJ, et al (1990) Regional distribution of body fat, plasma lipoproteins, and cardiovascular disease. Arteriosclerosis 10: 497-511. (Pubitemid 20220572)
25. Despres JP, Lemieux I. (2006) Adbominal obesity and metabolic syndrome. Nature 444: 881-887.
26. Wahrenberg H, Lonnqvist F, Arner P (1989) Mechanisms underlying regional differences in lipolysis in human adipose tissue. J Clin Invest 84: 458-467. (Pubitemid 19196976)
27. Arner P (1998) Not all fat is alike.Lancet 351:1301-1302. (Pubitemid 28195905)
28. Wajchenberg BL, Giannella-Neto D, da Silva MER, et al (2002) Depot-specific hormonal characteristics of subcutaneous and visceral adipose tissue and their relation to the metabolic syndrome. Horm Metab Res 34: 616-621. (Pubitemid 36398695)
29. Frayn KN (2000) Visceral fat and insulin resistance - causative or correlative? Br J Nutr 83 (suppl. 1); S71-77. (Pubitemid 30319085)
30. Fruhbeck G (2008) Overview of adipose tissue and its role in obesity and metabolic disorders. In: Yang K (ed) Methods in Molecular Biology: Adipose Tissue Protocols. 2nd edn. Humana Press.
31. Havel PJ (2004) Update on adipocyte hormones: regulation of energy balance and carbohydrate/ lipid metabolism. Diabetes 53(Suppl1): S143-S151. (Pubitemid 38168707)
32. Leibel RL, Hirsch J (1987) Site- and sex-related differences in adrenoreceptor status of human adipose tissue. J Clin Endocrinol Metab 64: 1205-1210. (Pubitemid 17074780)
33. Duserre E, Moulin P, Vidal H (2000) Differences in mRNA expression of the proteins secreted by the adipocytes in human subcutaneous and visceral adipose tissue. Biochim Biophys Acta 1500: 88-96. (Pubitemid 29525402)
34. LaFontan M, Berlan M (2003) Do regional differences in adipocyte biology provide new pathophysiological insights? Trends in Pharm Sci 24: 276-283. (Pubitemid 36725002)
35. Albu JB, Curi M, Shur M, et al (1999) Systemic resistance to the antilipolytic effect of insulin in black and white women with visceral obesity Am J Physiol 277: E551-E560. (Pubitemid 29458217)
36. Montague CT, O'Rahilly S (2000) The perils of portliness: causes and consequences of visceral adiposity. Diabetes 49: 883-888. (Pubitemid 30349408)
37. Fried SK, Bunkin DA, Greenberg AS (1998) Omental and subcutaneous adipose tissues of obese subjects release Interleukin-6: depot difference and regulation by glucocorticoid. J Clin Endocrinol Metab 83: 847-850. (Pubitemid 28496456)
38. Engfeldt P, Arner P (1988) Lipolysis in human adipocytes, effects of cell size, age and of regional differences. Horm Metab Res Suppl 19: 26-29. (Pubitemid 19005617)
39. Rosenbaum M, Presta E, Hirsch J et al (1991) Regional differences in adrenoreceptor status of adipose tissue in adults and prepubertal children. J Clin Endocrinol Metab 73: 341-7. (Pubitemid 21921879)
40. Zierath JR, Livingston JN, Thorne A et al (1998) Regional difference in insulin inhibition of non-esterified fatty acid release from human adipocytes: relation to insulin receptor phosphorylation and intracellular signalling through the insulin receptor substrate-1 pathway. Diabetologia 41: 1343-1354. (Pubitemid 28488763)
41. Maeda, K, Okubo K, Shimomura I et al (1997) Analysis of an expression profile of genes in the human adipose tissue. Gene 190: 227-235. (Pubitemid 27220127)
42. Arner P, Hellstrom L, Wahrenberg H et al (1990) Beta-adrenoceptor expression in human fat cells from different regions. J Clin Invest 86: 1595-1600. (Pubitemid 20372609)
43. Cigolini M, Smith U (1979) Human adipose tissue in culture: VII. Studies on the insulinantagonistic effect of glucocorticoids. Metabolism 28: 502.
44. Zhou X, Li D, Yin J et al (2007) CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle. J Lipid Res 48: 1701-1709. (Pubitemid 47311987)
45. Fernandez M, Acuna MJ, Reyes M et al (2001) Proliferation and differentiation of human adipocyte precursor cells: differences between the preperitoneal and subcutaneous compartments. J Cell Biochem 111: 659-664.
46. Sjostrom, Bjorntorp P, Vrana J (1971) Microscopic fat cell size measurements on frozen- cut adipose tissue in comparison with automatic determinations of osmium-fixed fat cells. J Lipid Res 12: 521-530.
47. Di Giralomo M, Mendlinger S, Fertig JW (1971) A simple method to determine fat cell size and number in four mammalian species. Am J Physiol 221: 850-858.
48. Smith U (1972) Studies of human adipose tissue in culture. 1. Incorporation of glucose and release of glycerol. Ant Rec 172: 597-602.