Single Cell Analysis of Yeast Replicative Aging Using a New Generation of Microfluidic Device

PLoS ONE

Volumn 7, Issue 11, 2012, Pages

  Zhang, Yi ^a,b   Luo, Chunxiong ^a   Zou, Ke ^a,b   Xie, Zhengwei ^a   Brandman, Onn ^b   Ouyang, Qi ^a   Li, Hao ^b  

^a PEKING UNIVERSITY   (China)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELONGATION FACTOR 1ALPHA; ELONGATION FACTOR 2; MOLECULAR MARKER;

AGE DISTRIBUTION; ARTICLE; CELL AGING; CELL BUDDING; CELL LINEAGE; CELL SIZE; CELL SURVIVAL; CELLULAR STRESS RESPONSE; DAUGHTER CELL; FLUORESCENCE; GENE EXPRESSION REGULATION; INHERITANCE; MARKER GENE; MICROFLUIDICS; NONHUMAN; PROCESS DEVELOPMENT; PROTEIN EXPRESSION; SINGLE CELL ANALYSIS; TRANSLATION ELONGATION FACTOR 2 GENE; TRANSLATION REGULATION; YEAST CELL;

BIOLOGICAL MARKERS; CALIBRATION; CELL DIVISION; CHITIN; GENE EXPRESSION; GENE EXPRESSION REGULATION, FUNGAL; GENES, REPORTER; LUMINESCENT PROTEINS; MICROFLUIDIC ANALYTICAL TECHNIQUES; PEPTIDE ELONGATION FACTORS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SINGLE-CELL ANALYSIS; STAINING AND LABELING; STRESS, PHYSIOLOGICAL;

EID: 84869051816     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0048275     Document Type: Article

References (29)

1. Smith ED, Tsuchiya M, Fox LA, Dang Nick, Hu D, et al. (2008) Quantitative evidence for conserved longevity pathways between divergent eukaryotic speicies. Genome Res 18: 564-570.
2. Mortimer RK, Johnston JR, (1959) Life span of individual yeast cells. Nature 183: 1751-1752.
3. Koschwanez J, Holl M, Carlson R, McMurray M, Gottschling D, et al. (2005) Automated lifetime analysis of a single yeast cell. Automation Science and Engineering. IEEE International Conference on 13-18. doi:10.1109/COASE.2005.1506738.
4. Ryley J, Pereira-Smith OM, (2006) Microfluidics device for single cell gene expression analysis in Saccharomyces cerevisiae. Yeast 23: 1065-1073.
5. Zhengwei X, Yi Z, Ke Z, Onn B, Chunxiong L, et al. (2012) Molecular Phenotyping of Aging in Single Yeast Cells Using a Novel Microfluidic Device. Aging Cell 11: 599-606.
6. Ballou CE. (1982) Yeast cell wall and cell surface. In The Molecular Biology of the Yeast Saccharomyces. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
7. Schekman R, Novick P. (1982) The secretary process and yeast cell-surface assembly. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
8. Tod S, James C, Brian K, Francesca C, Leonard G, (1996) Loss of Transcriptional Silencing Causes Sterility in Old Mother Cells of S. Cerevisiae. Cell 84 (4): 633-642.
9. Sung SL, Ima AV, Daphne HH, Luke PL, Matthias H, (2012) Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform. PNAS 109 (13): 4916-4920.
10. Chunxiong L, Lingli J, Shibo L, Qi O, Hang J, et al. (2009) High-throughput microfluidic system for monitoring diffusion based monolayer yeast cell culture over long time periods. Biomed Microdevices 11: 981-986.
11. Steffen KK, Kennedy BK, Kaeberlein M, (2009) Measuring replicative lifespan in the budding yeast. J Vis Exp (28) pp. 1209.
12. Kennedy BK, Austriaco NR Jr, Guarente L, (1994) Daughter cells of saccharomyces cerevisiae from old mothers display a reduced life span. J Cell Biol 127 (6): 1985-1993.
13. Vanoni M, Vai M, Popolo L, Alberghina L, (1983) Structural heterogeneity populations of the budding yeast saccharomyces cerevisiae. J Bacteriol 156 (3): 1282-1291.
14. Powell CD, Quain DE, Smart KA, (2003) Chitin scar breaks in aged saccharomyces cerevisiae. Microbiology 149: 3129-3137.
15. Kaeberlein M, McVey M, Guarente L, (1999) The SIR2/3/4 complex and SIR2 alone promote longevity in saccharomyces cerevisiae by two different mechanisms. Gene Dev 13 (19): 2570-2580.
16. Defossez PA, Prusty R, Kaeberlein M, Lin S, Ferrigno P, et al. (1999) Elimination of replication block protein Fob1 extends the life span of yeast mother cells. Mol Cell 3 (4): 447-455.
17. Kaeberlein M, Kirkland TK, Fields S, Kennedy BK, (2005) Genes determining yeast replicative life span in a long-lived genetic background. Mech Ageing Dev 126: 491-504.
18. Shcheprova Z, Baldi S, Frei SB, Gonnet G, Barral Y, (2008) A mechanism for asymmetric segregation of age during yeast budding. Nature 454: 728-734.
19. Aguilaniu H, Gustafsson L, Rigoulet M, Nystrom T, (2003) Asymmetric inheritance of oxidatively damaged proteins during cytokinesis. Science 299: 1751-1753.
20. Lai CY, Jaruga E, Borghouts C, Jazwinski SM, (2008) A mutation in the ATP2gene abrogates the age asymmetry between mother and daughter cells of the yeast saccharomyces cerevisiae. Genetics 162: 73-87.
21. Boy Marcotte E, Perrot M, Bussereau F, Boucherie H, Jacquet M, (1998) Msn2p and Msn4p control a large number of genes induced at the diauxic transition which are repressed by cyclic AMP in saccharomyces cerevisiae. J Bacteriol 180: 1044-1052.
22. Garreau H, Hasan RN, Renault G, Estruch F, Boy Marcotte E, et al. (2000) Hyperphosphorylation of Msn2p and Msn4p in response to heat shock and the diauxic shift is inhibited by cAMP in saccharomyces cerevisiae. Microbiology 146: 2113-2120.
23. De Wever V, Reiter W, Ballarini A, Ammerer G, Brocard C, (2005) A dual role for PP1 in shaping the Msn2-dependent transcriptional response to glucose starvation. EMBO J 24: 4115-4123.
24. Gasch AP, Spellman PT, Kao CM, Carmel HO, Eisen BM, et al. (2000) Genomic expression programs in the response of yeast cells to environmental changes. Mol Biol Cell 11: 4241-4257.
25. Boy Marcotte E, Lagniel G, Perrot M, Bussereau F, Boudsocq A, et al. (1999) The heat shock response in yeast: differential regulations and contributions of the Msn2p/Msn4p and Hsf1p regulons. Mol. Microbiol 33: 274-283.
26. Klinger H, Rinnerthaler M, Lam YT, Laun P, Heeren G, et al. (2010) Quantitation of (a)symmetric inheritance of functional and of oxidatively damaged mitochondrial aconitase in the cell division of old yeast mother cells. Experimental Gerontology 45: 533-542.
27. Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425: 686-91.
28. Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, et al. (2007) Bright far-red fluorescent protein for whole-body imaging. Nat Methods 4 (9): 741-746.
29. Shchcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, et al. (2009) Far-red fluorescent tags for protein imaging in living tissues. Biochem J 418 (3): 567-574.