Measuring replicative life span in the budding yeast

Journal of Visualized Experiments

Volumn , Issue 28, 2009, Pages

  Steffen, Kristan K ^a   Kennedy, Brian K ^a   Kaeberlein, Matt ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Aging; Developmental biology; Dietary restriction; Issue 28; Life span; Longevity; Saccharomyces cerevisiae; Yeast

Indexed keywords

EID: 80055114735     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1209     Document Type: Article

References (10)

1. Kaeberlein, M., Burtner, C.R. & Kennedy, B.K. Recent developments in yeast aging. PLoS Genet 3, e84 (2007).
2. Kaeberlein, M. in Handbook of models for human aging (ed. Conn, P.M.) 109-120 (Elsevier Press, Boston, 2006).
3. Smith, E.D. et al. Quantitative evidence for conserved longevity pathways between divergent eukaryotic species. Genome Res 18, 564-570 (2008).
4. Steinkraus, K.A., Kaeberlein, M. & Kennedy, B.K. Replicative aging in yeast: the means to the end. Annu Rev Cell Dev Biol 24, 29-54 (2008).
5. Murakami, C.J., Burtner, C.R., Kennedy, B.K. & Kaeberlein, M. A method for high-throughput quantitative analysis of yeast chronological life span. J Gerontol A Biol Sci Med Sci 63, 113-121 (2008).
6. Jiang, J.C., Jaruga, E., Repnevskaya, M.V. & Jazwinski, S.M. An intervention resembling caloric restriction prolongs life span and retards aging in yeast. Faseb J 14, 2135-2137 (2000).
7. Kaeberlein, M., Kirkland, K.T., Fields, S. & Kennedy, B.K. Sir2-independent life span extension by calorie restriction in yeast. PLoS Biol 2, E296 (2004).
8. Lin, S.J., Defossez, P.A. & Guarente, L. Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae. Science 289, 2126-2128. (2000).
9. Kaeberlein, M. & Kennedy, B.K. Large-scale identification in yeast of conserved ageing genes. Mech Ageing Dev 126, 17-21 (2005).
10. Kaeberlein, M. et al. Regulation of yeast replicative life span by TOR and Sch9 in response to nutrients. Science 310, 1193-1196 (2005).