Expression, purification, crystallization and preliminary X-ray diffraction analysis of the dihydroorotase domain of human CAD

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 68, Issue 11, 2012, Pages 1341-1345

  Lallous, Nada ^a   Grande García, Araceli ^a   Molina, Rafael ^a   Ramón Maiques, Santiago ^a  

^a Centro Nacional de Investigaciones Oncológicas   (Spain)

Author keywords

dihydroorotase domain; human CAD; pyrimidine biosynthesis

Indexed keywords

ASPARTATE CARBAMOYLTRANSFERASE; CAD TRIFUNCTIONAL ENZYME; CARBAMOYL PHOSPHATE SYNTHASE (GLUTAMINE HYDROLYSING); DIHYDROOROTASE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CRYSTALLIZATION; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; LIGHT; PROTEIN QUATERNARY STRUCTURE; RADIATION SCATTERING; X RAY CRYSTALLOGRAPHY;

ASPARTATE CARBAMOYLTRANSFERASE; CARBAMOYL-PHOSPHATE SYNTHASE (GLUTAMINE-HYDROLYZING); CATALYTIC DOMAIN; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DIHYDROOROTASE; ESCHERICHIA COLI; HUMANS; LIGHT; PROTEIN STRUCTURE, QUATERNARY; SCATTERING, RADIATION;

EUKARYOTA; PROKARYOTA;

EID: 84869033524     PISSN: None     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S1744309112038857     Document Type: Article

References (33)

1. Ahuja, A., Purcarea, C., Ebert, R., Sadecki, S., Guy, H. I. & Evans, D. R. (2004). J. Biol. Chem. 279, 53136-53144.
2. Aoki, T. & Weber, G. (1981). Science, 212, 463-465.
3. Christopherson, R. I., Lyons, S. D. & Wilson, P. K. (2002). Acc. Chem. Res. 35, 961-971.
4. Coleman, P. F., Suttle, D. P. & Stark, G. R. (1977). J. Biol. Chem. 252, 6379- 6385.
5. Davidson, J. N., Chen, K. C., Jamison, R. S., Musmanno, L. A. & Kern, C. B. (1993). Bioessays, 15, 157-164.
6. Evans, D. R. & Guy, H. I. (2004). J. Biol. Chem. 279, 33035-33038.
7. Fairbanks, L. D., Bofill, M., Ruckemann, K. & Simmonds, H. A. (1995). J. Biol. Chem. 270, 29682-29689.
8. Fields, C., Brichta, D., Shephardson, M., Farinha, M. & O'Donovan, G. (1999). Paths Pyrimidines, 7, 49-63.
9. Gero, A. M., Brown, G. V. & O'Sullivan,W. J. (1984). J. Parasitol. 70, 536-541.
10. Holm, L. & Sander, C. (1997). Proteins, 28, 72-82.
11. Jones, M. E. (1980). Annu. Rev. Biochem. 49, 253-279.
12. Kabsch, W. (2010). Acta Cryst. D66, 125-132.
13. Kelly, R. E., Mally, M. I. & Evans, D. R. (1986). J. Biol. Chem. 261, 6073-6083.
14. Kim, H., Kelly, R. E. & Evans, D. R. (1992). J. Biol. Chem. 267, 7177-7184.
15. Krungkrai, J., Cerami, A. & Henderson, G. B. (1990). Biochemistry, 29, 6270- 6275.
16. Lee, L., Kelly, R. E., Pastra-Landis, S. C. & Evans, D. R. (1985). Proc. Natl Acad. Sci. USA, 82, 6802-6806.
17. Lee, M., Chan, C. W., Graham, S. C., Christopherson, R. I., Guss, J. M. & Maher, M. J. (2007). J. Mol. Biol. 370, 812-825.
18. Lee, M., Chan, C. W., Guss, J. M., Christopherson, R. I. & Maher, M. J. (2005). J. Mol. Biol. 348, 523-533.
19. Lollier, M., Jaquet, L., Nedeva, T., Lacroute, F., Potier, S. & Souciet, J.-L. (1995). Curr. Genet. 28, 138-149.
20. Maher, M. J., Huang, D. T. C., Guss, J. M., Collyer, C. A. & Christopherson, R. I. (2003). Acta Cryst. D59, 381-384.
21. Martin, P. D., Purcarea, C., Zhang, P., Vaishnav, A., Sadecki, S., Guy-Evans, H. I., Evans, D. R. & Edwards, B. F. (2005). J. Mol. Biol. 348, 535-547.
22. Musmanno, L. A., Jamison, R. S., Barnett, R. S., Buford, E. & Davidson, J. N. (1992). Somat. Cell Mol. Genet. 18, 309-318.
23. Porter, T. N., Li, Y. & Raushel, F. M. (2004). Biochemistry, 43, 16285- 16292.
24. Sigoillot, F. D., Kotsis, D. H., Serre, V., Sigoillot, S. M., Evans, D. R. & Guy, H. I. (2005). J. Biol. Chem. 280, 25611-25620.
25. Sigoillot, F. D., Sigoillot, S. M. & Guy, H. I. (2004). Int. J. Cancer, 109, 491-498.
26. Simmer, J. P., Kelly, R. E., Rinker, A. G., Scully, J. L. & Evans, D. R. (1990). J. Biol. Chem. 265, 10395-10402.
27. Souciet, J.-L., Nagy, M., Le Gouar, M., Lacroute, F. & Potier, S. (1989). Gene, 79, 59-70.
28. Studier, F. W. (2005). Protein Expr. Purif. 41, 207-234.
29. Thoden, J. B., Phillips, G. N., Neal, T. M., Raushel, F. M. & Holden, H. M. (2001). Biochemistry, 40, 6989-6997.
30. Williams, N. K., Peide, Y., Seymour, K. K., Ralston, G. B. & Christopherson, R. I. (1993). Protein Eng. 6, 333-340.
31. Wyatt, P. J. (1993). Anal. Chim. Acta, 272, 1-40.
32. Zhang, P., Martin, P. D., Purcarea, C., Vaishnav, A., Brunzelle, J. S., Fernando, R., Guy-Evans, H. I., Evans, D. R. & Edwards, B. F. (2009). Biochemistry, 48, 766-778.
33. Zrenner, R., Stitt, M., Sonnewald, U. & Boldt, R. (2006). Annu. Rev. Plant Biol. 57, 805-836.