Optimization of one-step duplex real-time RT-PCR for detection of influenza and respiratory syncytial virus in nasopharyngeal aspirates

Journal of Virological Methods

Volumn 186, Issue 1-2, 2012, Pages 189-192

  De Paris, Fernanda ^a,b   Beck, Caroline ^b   Machado, Alice Beatriz Mombach Pinheiro ^a   Paiva, Rodrigo Minuto ^a   da Silva Menezes, Denise ^a   de Souza Nunes, Luciana ^b   Kuchenbecker, Ricardo ^b   Barth, Afonso Luís ^a,b  

^a HOSPITAL DE CLÍNICAS DE PORTO ALEGRE   (Brazil)

^b FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

Author keywords

Influenza virus; Molecular diagnostics; Molecular epidemiology; One step real time PCR; Respiratory syncytial virus

Indexed keywords

HEMAGGLUTININ; MATRIX PROTEIN; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; HUMAN; IMMUNOFLUORESCENCE; INFLUENZA A; INFLUENZA B; INFLUENZA VIRUS; INFLUENZA VIRUS A; INFLUENZA VIRUS B; LIMIT OF DETECTION; MOLECULAR DIAGNOSIS; N GENE; NASOPHARYNGEAL ASPIRATION; NONHUMAN; ONE STEP DUPLEX REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; OUTCOME ASSESSMENT; PRIORITY JOURNAL; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RESPIRATORY SYNCYTIAL VIRUS INFECTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA EXTRACTION; SENSITIVITY ANALYSIS; VIRUS DETECTION; VIRUS GENE;

DNA PRIMERS; HUMANS; INFLUENZA, HUMAN; MULTIPLEX POLYMERASE CHAIN REACTION; NASOPHARYNX; ORTHOMYXOVIRIDAE; REAL-TIME POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; RESPIRATORY TRACT INFECTIONS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

HUMAN RESPIRATORY SYNCYTIAL VIRUS; ORTHOMYXOVIRIDAE; RESPIRATORY SYNCYTIAL VIRUS;

EID: 84869016256     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.07.008     Document Type: Article

References (15)

1. Brittain-Long R., Westin J., Olofsson S., Lindh M., Andersson L.M. Prospective evaluation of a novel multiplex real-time PCR assay for detection of fifteen respiratory pathogens-duration of symptoms significantly affects detection rate. Journal of Clinical Virology 2010, 47:263-267.
2. Caram L.B., Chen J., Taggart E.W., Hillyard D.R., She R., Polage C.R., Twersky J., Schmader K., Petti C.A., Woods C.W. Respiratory syncytial virus outbreak in a long-term care facility detected using reverse transcriptase polymerase chain reaction: an argument for real-time detection methods. Journal of the American Geriatrics Society 2009, 57:482-485.
3. Fairchok M.P., Martin E.T., Chambers S., Kuypers J., Behrens M., Braun L.E., Englund J.A. Epidemiology of viral respiratory tract infections in a prospective cohort of infants and toddlers attending daycare. Journal of Clinical Virology 2010, 49:16-20.
4. Falsey A.R., Hennessey P.A., Formica M.A., Cox C., Walsh E.E. Respiratory syncytial virus infection in elderly and high-risk adults. New England Journal of Medicine 2005, 352:1749-1759.
5. Gatherer D. The 2009 H1N1 influenza outbreak in its historical context. Journal of Clinical Virology 2009, 45:174-178.
6. Hall C.B., Weinberg G.A., Iwane M.K., Blumkin A.K., Edwards K.M., Staat M.A., Auinger P., Griffin M.R., Poehling K.A., Erdman D., Grijalva C.G., Zhu Y., Szilagyi P. The burden of respiratory syncytial virus infection in young children. New England Journal of Medicine 2009, 360:588-598.
7. Hu A., Colella M., Tam J.S., Rappaport R., Cheng S. Simultaneous detection, subgrouping, and quantitation of respiratory syncytial virus A and B by real-time PCR. Journal of Clinical Microbiology 2003, 41:149-154.
8. Kehl S.C., Kumar S. Utilization of nucleic acid amplification assays for the detection of respiratory viruses. Clinics in Laboratory Medicine 2009, 29:661-671.
9. Kuypers J., Wright N., Ferrenberg J., Huang M.L., Cent A., Corey L., Morrow R. Comparison of real-time PCR assays with fluorescent-antibody assays for diagnosis of respiratory virus infections in children. Journal of Clinical Microbiology 2006, 44:2382-2388.
10. Olofsson S., Brittain-Long R., Andersson L.M., Westin J., Lindh M. PCR for detection of respiratory viruses: seasonal variations of virus infections. Expert Review of Anti Infective Therapy 2011, 9:615-626.
11. Panning M., Eickmann M., Landt O., Monazahian M., Olschläger S., Baumgarte S., Reischl U., Wenzel J.J., Niller H.H., Günther S., Hollmann B., Huzly D., Drexler J.F., Helmer A., Becker S., Matz B., Eis-Hübinger A., Drosten C. Detection of influenza A (H1N1)v virus by real-time RT-PCR. Euro Surveillance 2009, 14:1-6.
12. Paranhos-Baccalà G., Komurian-Pradel F., Richard N., Vernet G., Lina B., Floret D. Mixed respiratory virus infections. Journal of Clinical Virology 2008, 43:407-410.
13. Richard N., Komurian-Pradel F., Javouhey E., Perret M., Rajoharison A., Bagnaud A., Billaud G., Vernet G., Lina B., Floret D., Paranhos-Baccalà G. The impact of dual viral infection in infants admitted to a pediatric intensive care unit associated with severe bronchiolitis. Pediatric Infectious Disease Journal 2008, 27:213-217.
14. Schweiger B., Zadow I., Heckler R., Timm H., Pauli G. Application of a fluorogenic PCR assay for typing and subtyping of influenza viruses in respiratory samples. Journal of Clinical Microbiology 2000, 38:1552-1558.
15. World Health Organization CDC Protocol of Real Time RT-PCR for Swine Influenza A (H1N1) 2009, WHO, Atlanta.