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Tiveljung-Lindell A, Rotzen-Ostlund M, Gupta S et al. Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses. J. Med. Virol. 81(1), 167-175 (2009). Presentation of a large molecular diagnostic platform with the capacity to replace direct immunofluorescence in all clinically relevant situations.
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Sadeghi CD, Aebi C, Gorgievski-Hrisoho M, Muhlemann K, Barbani MT. Twelve years' detection of respiratory viruses by immunofluorescence in hospitalised children: Impact of the introduction of a new respiratory picornavirus assay. BMC Infect. Dis. 11, 41 (2011).
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Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay
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Mahony J, Chong S, Merante F et al. Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay. J. Clin. Microbiol. 45(9), 2965-2970 (2007). (Pubitemid 47417341)
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Important study comparing the performance of direct fluorescent antibody with molecular techniques for respiratory syncytial virus and human metapneumovirus
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Jokela P, Piiparinen H, Luiro K, Lappalainen M. Detection of human metapneumovirus and respiratory syncytial virus by duplex real-time RT-PCR assay in comparison with direct fluorescent assay. Clin. Microbiol. Infect. 16(10), 1568-1573 (2010). Important study comparing the performance of direct fluorescent antibody with molecular techniques for respiratory syncytial virus and human metapneumovirus.
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Templeton KE, Scheltinga SA, Beersma MF, Kroes AC, Claas EC. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4. J. Clin. Microbiol. 42(4), 1564-1569 (2004). (Pubitemid 38500797)
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Antonishyn NA, Levett PN. Molecular diagnostic assays for detection of viral respiratory pathogens in institutional outbreaks. Mol. Diagn. Ther. 14(5), 283-293 (2010). Excellent review primarily discussing diagnostic preparedness regarding emerging respiratory viruses, focusing on the versatility of molecular tests in this context.
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