Prospective evaluation of a novel multiplex real-time PCR assay for detection of fifteen respiratory pathogens-Duration of symptoms significantly affects detection rate

Journal of Clinical Virology

Volumn 47, Issue 3, 2010, Pages 263-267

  Brittain Long, Robin ^a   Westin, Johan ^a   Olofsson, Sigvard ^a   Lindh, Magnus ^a   Andersson, Lars Magnus ^a  

^a SAHLGRENSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Multiplex PCR; Respiratory tract infection; Respiratory viruses

Indexed keywords

ADULT; AGED; ARTICLE; BACTERIUM DETECTION; CHLAMYDOPHILA; COMMUNITY ACQUIRED PNEUMONIA; CONTROLLED STUDY; CORONAVIRUS; FEMALE; FOLLOW UP; HUMAN; INFLUENZA VIRUS A; INFLUENZA VIRUS B; MAJOR CLINICAL STUDY; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOPLASMA; NONHUMAN; NOSE SMEAR; PRIORITY JOURNAL; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY TRACT INFECTION; VIRUS DETECTION; VIRUS INFECTION; VIRUS LOAD;

ADULT; AGED; AGED, 80 AND OVER; BACTERIA; BACTERIAL INFECTIONS; COMMUNITY-ACQUIRED INFECTIONS; FEMALE; HUMANS; MALE; MIDDLE AGED; NASOPHARYNX; POLYMERASE CHAIN REACTION; PROSPECTIVE STUDIES; RESPIRATORY TRACT INFECTIONS; TIME FACTORS; VIRUS DISEASES; VIRUSES; YOUNG ADULT;

EID: 76449098508     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcv.2009.12.010     Document Type: Article

References (30)

1. Bellau-Pujol S., Vabret A., Legrand L., Dina J., Gouarin S., Petitjean-Lecherbonnier J., et al. Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. J Virol Methods 126 1-2 (2005 Jun) 53-63
2. Tiveljung-Lindell A., Rotzen-Ostlund M., Gupta S., Ullstrand R., Grillner L., Zweygberg-Wirgart B., et al. Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses. J Med Virol 81 January (1) (2009) 167-175
3. Gunson R.N., Collins T.C., and Carman W.F. Practical experience of high throughput real time PCR in the routine diagnostic virology setting. J Clin Virol 35 April (4) (2006) 355-367
4. Garbino J., Gerbase M.W., Wunderli W., Kolarova L., Nicod L.P., Rochat T., et al. Respiratory viruses and severe lower respiratory tract complications in hospitalized patients. Chest 125 March (3) (2004) 1033-1039
5. Lee B.E., Robinson J.L., Khurana V., Pang X.L., Preiksaitis J.K., and Fox J.D. Enhanced identification of viral and atypical bacterial pathogens in lower respiratory tract samples with nucleic acid amplification tests. J Med Virol 78 May (5) (2006) 702-710
6. Oosterheert J.J., van Loon A.M., Schuurman R., Hoepelman A.I., Hak E., Thijsen S., et al. Impact of rapid detection of viral and atypical bacterial pathogens by real-time polymerase chain reaction for patients with lower respiratory tract infection. Clin Infect Dis 41 November (10) (2005) 1438-1444
7. Kaye M., Skidmore S., Osman H., Weinbren M., and Warren R. Surveillance of respiratory virus infections in adult hospital admissions using rapid methods. Epidemiol Infect 134 August (4) (2006) 792-798
8. Legoff J., Guerot E., Ndjoyi-Mbiguino A., Matta M., Si-Mohamed A., Gutmann L., et al. High prevalence of respiratory viral infections in patients hospitalized in an intensive care unit for acute respiratory infections as detected by nucleic acid-based assays. J Clin Microbiol 43 January (1) (2005) 455-457
9. Mahony J.B. Detection of respiratory viruses by molecular methods. Clin Microbiol Rev 21 October (4) (2008) 716-747
10. Renwick N., Schweiger B., Kapoor V., Liu Z., Villari J., Bullmann R., et al. A recently identified rhinovirus genotype is associated with severe respiratory-tract infection in children in Germany. J Infect Dis 196 December (12) (2007) 1754-1760
11. Louie J.K., Roy-Burman A., Guardia-Labar L., Boston E.J., Kiang D., Padilla T., et al. Rhinovirus associated with severe lower respiratory tract infections in children. Pediatr Infect Dis J 28 April (4) (2009) 337-339
12. Lam W.Y., Yeung A.C., Tang J.W., Ip M., Chan E.W., Hui M., et al. Rapid multiplex nested PCR for detection of respiratory viruses. J Clin Microbiol 45 November (11) (2007) 3631-3640
13. Wallace L.A., McAulay K.A., Douglas J.D., Elder A.G., Stott D.J., and Carman W.F. Influenza diagnosis: from dark isolation into the molecular light West of Scotland Respiratory Virus Study Group. J Infect 39 November (3) (1999) 221-226
14. Brittain-Long R., Nord S., Olofsson S., Westin J., Anderson L.M., and Lindh M. Multiplex real-time PCR for detection of respiratory tract infections. J Clin Virol 41 January (1) (2008) 53-56
15. Leekha S., Zitterkopf N.L., Espy M.J., Smith T.F., Thompson R.L., and Sampathkumar P. Duration of influenza A virus shedding in hospitalized patients and implications for infection control. Infect Cntrl Hosp Epidemiol 28 September (9) (2007) 1071-1076
16. Creer D.D., Dilworth J.P., Gillespie S.H., Johnston A.R., Johnston S.L., Ling C., et al. Aetiological role of viral and bacterial infections in acute adult lower respiratory tract infection (LRTI) in primary care. Thorax 61 January (1) (2006) 75-79
17. Mahony J., Chong S., Merante F., Yaghoubian S., Sinha T., Lisle C., et al. Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay. J Clin Microbiol 45 September (9) (2007) 2965-2970
18. Ruohola A., Waris M., Allander T., Ziegler T., Heikkinen T., and Ruuskanen O. Viral etiology of common cold in children, Finland. Emerg Infect Dis 15 February (2) (2009) 344-346
19. Freymuth F., Vabret A., Cuvillon-Nimal D., Simon S., Dina J., Legrand L., et al. Comparison of multiplex PCR assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness. J Med Virol 78 November (11) (2006) 1498-1504
20. Nokso-Koivisto J., Kinnari T.J., Lindahl P., Hovi T., and Pitkaranta A. Human picornavirus and coronavirus RNA in nasopharynx of children without concurrent respiratory symptoms. J Med Virol 66 March (3) (2002) 417-420
21. van Benten I., Koopman L., Niesters B., Hop W., van Middelkoop B., de Waal L., et al. Predominance of rhinovirus in the nose of symptomatic and asymptomatic infants. Pediatr Allergy Immunol 14 October (5) (2003) 363-370
22. Jartti T., Lehtinen P., Vuorinen T., Koskenvuo M., and Ruuskanen O. Persistence of rhinovirus and enterovirus RNA after acute respiratory illness in children. J Med Virol 72 April (4) (2004) 695-699
23. Templeton K.E., Scheltinga S.A., Beersma M.F., Kroes A.C., and Claas E.C. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza A and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4. J Clin Microbiol 42 April (4) (2004) 1564-1569
24. Ison M.G. Respiratory viral infections in transplant recipients. Antivir Ther 4 Pt. B (2007) 627-638
25. Peck A.J., Englund J.A., Kuypers J., Guthrie K.A., Corey L., Morrow R., et al. Respiratory virus infection among hematopoietic cell transplant recipients: evidence for asymptomatic parainfluenza virus infection. Blood 110 September (5) (2007) 1681-1688
26. Gerna G., Campanini G., Rognoni V., Marchi A., Rovida F., Piralla A., et al. Correlation of viral load as determined by real-time RT-PCR and clinical characteristics of respiratory syncytial virus lower respiratory tract infections in early infancy. J Clin Virol 41 January (1) (2008) 45-48
27. Campanini G., Percivalle E., Baldanti F., Rovida F., Bertaina A., Marchi A., et al. Human respiratory syncytial virus (hRSV) RNA quantification in nasopharyngeal secretions identifies the hRSV etiologic role in acute respiratory tract infections of hospitalized infants. J Clin Virol 39 June (2) (2007) 119-124
28. Chung J.Y., Han T.H., Kim S.W., and Hwang E.S. Respiratory picornavirus infections in Korean children with lower respiratory tract infections. Scand J Infect Dis 39 3 (2007) 250-254
29. Pierangeli A., Gentile M., Di Marco P., Pagnotti P., Scagnolari C., Trombetti S., et al. Detection and typing by molecular techniques of respiratory viruses in children hospitalized for acute respiratory infection in Rome, Italy. J Med Virol 79 April (4) (2007) 463-468
30. Calvo C., Garcia-Garcia M.L., Blanco C., Vazquez M.C., Frias M.E., Perez-Brena P., et al. Multiple simultaneous viral infections in infants with acute respiratory tract infections in Spain. J Clin Virol 42 July (3) (2008) 268-272