Toxicity of bovicin HC5 against mammalian cell lines and the role of cholesterol in bacteriocin activity

Microbiology (United Kingdom)

Volumn 158, Issue 11, 2012, Pages 2851-2858

  Paiva, Aline Dias ^a   de Oliveira, Michelle Dias ^a   de Paula, Sérgio Oliveira ^a   Baracat Pereira, Maria Cristina ^a   Breukink, Eefjan ^b   Mantovani, Hilário Cuquetto ^a  

^a FEDERAL UNIVERSITY OF VIÇOSA   (Brazil)

^b UTRECHT UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; BACTERIOCIN; BOVICIN HC5; CARBOXYFLUORESCEIN; CHOLESTEROL; DIOLEOYLPHOSPHATIDYLCHOLINE; HEMOGLOBIN; LANTIBIOTIC; NISIN; UNCLASSIFIED DRUG;

ARTICLE; CELL LINE; CELL STRAIN HEPG2; CELL STRAIN MCF 7; CONTROLLED STUDY; DRUG CYTOTOXICITY; EUKARYOTIC CELL; HEMOLYSIS; HUMAN; HUMAN CELL; IC 50; IN VITRO STUDY; MAMMAL CELL; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; VERO CELL;

ANIMALS; BACTERIOCINS; CELL LINE; CELL SURVIVAL; CERCOPITHECUS AETHIOPS; CHOLESTEROL; EUKARYOTIC CELLS; HEMOLYTIC AGENTS; HUMANS; VERO CELLS;

EUKARYOTA; MAMMALIA;

EID: 84868326115     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.062190-0     Document Type: Article

References (35)

1. Begde, D., Bundale, S., Mashitha, P., Rudra, J., Nashikkar, N. & Upadhyay, A. (2011). Immunomodulatory efficacy of nisin-a bacterial lantibiotic peptide. J Pept Sci 17, 438-444.
2. Benachir, T., Monette, M., Grenier, J. & Lafleur, M. (1997). Melittininduced leakage from phosphatidylcholine vesicles is modulated by cholesterol: a property used for membrane targeting. Eur Biophys J Biophys Lett 25, 201-210.
3. Berridge, V. M. & Tan, S. A. (1993). Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondria electron transport in MTT reduction. Arch Biochem Biophys 303, 474-482.
4. Brender, J. R., McHenry, A. J. & Ramamoorthy, A. (2012). Does cholesterol play a role in the bacterial selectivity of antimicrobial peptides? Front Immunol 3, 195.
5. Breukink, E., Wiedemann, I., van Kraaij, C., Kuipers, O. P., Sahl, H. & de Kruijff, B. (1999). Use of the cell wall precursor lipid II by a poreforming peptide antibiotic. Science 286, 2361-2364.
6. Breukink, E., van Heusden, H. E., Vollmerhaus, P. J., Swiezewska, E., Brunner, L., Walker, S., Heck, A. J. R. & de Kruijff, B. (2003). Lipid II is an intrinsic component of the pore induced by nisin in bacterial membranes. J Biol Chem 278, 19898-19903.
7. Cleveland, J., Montville, T. J., Nes, I. F. & Chikindas, M. L. (2001). Bacteriocins: safe, natural antimicrobials for food preservation. Int J Food Microbiol 71, 1-20.
8. Fotakis, G. & Timbrell, J. A. (2006). In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 160, 171-177.
9. Hetz, C., Bono, M. R., Barros, L. F. & Lagos, R. (2002). Microcin E492, a channel-forming bacteriocin from Klebsiella pneumoniae, induces apoptosis in some human cell lines. Proc Natl Acad Sci U S A 99, 2696-2701.
10. Hope, M. J., Bally, M. B., Webb, G. & Cullis, P. R. (1985). Production of large unilamellar vesicles by a rapid extrusion procedure-characterization of size distribution, trapped volume and ability to maintain a membrane-potential. Biochim Biophys Acta 812, 55-65.
11. Joerger, R. D. (2003). Alternatives to antibiotics: bacteriocins, antimicrobial peptides and bacteriophages. Poult Sci 82, 640-647.
12. Lewus, C. B. & Montville, T. J. (1991). Detection of bacteriocins produced by lactic acid bacteria. J Microbiol Methods 13, 145-150.
13. Lima, J. R., Ribon, A. O., Russell, J. B. & Mantovani, H. C. (2009). Bovicin HC5 inhibits wasteful amino acid degradation by mixed ruminal bacteria in vitro. FEMS Microbiol Lett 292, 78-84.
14. Maher, S. & McClean, S. (2006). Investigation of the cytotoxicity of eukaryotic and prokaryotic antimicrobial peptides in intestinal epithelial cells in vitro. Biochem Pharmacol 71, 1289-1298.
15. Mantovani, H. C. & Russell, J. B. (2003). Inhibition of Listeria monocytogenes by bovicin HC5, a bacteriocin produced by Streptococcus bovis HC5. Int J Food Microbiol 89, 77-83.
16. Mantovani, H. C., Hu, H., Worobo, R. W. & Russell, J. B. (2002). Bovicin HC5, a bacteriocin from Streptococcus bovis HC5. Microbiology 148, 3347-3352.
17. Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65, 55-63.
18. Mouritsen, O. G. & Zuckermann, M. J. (2004). What's so special about cholesterol? Lipids 39, 1101-1113.
19. Paiva, A. D., Breukink, E. & Mantovani, H. C. (2011). Role of lipid II and membrane thickness in the mechanism of action of the lantibiotic bovicin HC5. Antimicrob Agents Chemother 55, 5284-5293.
20. Paiva, A. D., Irving, N., Breukink, E. & Mantovani, H. C. (2012). Interaction with lipid II induces conformational changes in bovicin HC5 structure. Antimicrob Agents Chemother 56, 4586-4593.
21. Prenner, E. J., Lewis, R. N., Jelokhani-Niaraki, M., Hodges, R. S. & McElhaney, R. N. (2001). Cholesterol attenuates the interaction of the antimicrobial peptide gramicidin S with phospholipid bilayer membranes. Biochim Biophys Acta 1510, 83-92.
22. Raghuraman, H. & Chattopadhyay, A. (2004). Interaction of melittin with membrane cholesterol: a fluorescence approach. Biophys J 87, 2419-2432.
23. Raghuraman, H. & Chattopadhyay, A. (2005). Cholesterol inhibits the lytic activity of melittin in erythrocytes. Chem Phys Lipids 134, 183-189.
24. Raghuraman, H. & Chattopadhyay, A. (2007). Orientation and dynamics of melittin in membranes of varying composition utilizing NBD fluorescence. Biophys J 92, 1271-1283.
25. Reddy, K. V. R., Yedery, R. D. & Aranha, C. (2004). Antimicrobial peptides: premises and promises. Int J Antimicrob Agents 24, 536-547.
26. Rouser, G., Fleischer, S. & Yamamoto, A. (1970). Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids 5, 494-496.
27. Shin, S. Y., Yang, S.-T., Park, E. J., Eom, S. H., Song, W. K., Kim, J. I., Lee, S.-H., Lee, M. K., Lee, D. G. & other authors (2001). Antibacterial, antitumor and hemolytic activities of alpha-helical antibiotic peptide, P18 and its analogs. J Pept Res 58, 504-514.
28. Tagg, J. R., Dajani, A. S. & Wannamaker, L. W. (1976). Bacteriocins of gram-positive bacteria. Bacteriol Rev 40, 722-756.
29. Thomson, C. J., Power, E., Ruebsamen-Waigmann, H. & Labischinski, H. (2004). Antibacterial research and development in the 21st Century-an industry perspective of the challenges. Curr Opin Microbiol 7, 445-450.
30. Todorov, S. D., Wachsman, M. B., Knoetze, H., Meincken, M. & Dicks, L. M. T. (2005). An antibacterial and antiviral peptide produced by Enterococcus mundtii ST4V isolated from soya beans. Int J Antimicrob Agents 25, 508-513.
31. Vaucher, R. A., Teixeira, M. L. & Brandelli, A. (2010). Investigation of the cytotoxicity of antimicrobial peptide P40 on eukaryotic cells. Curr Microbiol 60, 1-5.
32. Verly, R. M., Rodrigues, M. A., Daghastanli, K. R., Denadai, A. M., Cuccovia, I. M., Bloch, C., Jr, Frézard, F., Santoro, M. M., Piló-Veloso, D. & Bemquerer, M. P. (2008). Effect of cholesterol on the interaction of the amphibian antimicrobial peptide DD K with liposomes. Peptides 29, 15-24.
33. Wessman, P., Strömstedt, A. A., Malmsten, M. & Edwards, K. (2008). Melittin-lipid bilayer interactions and the role of cholesterol. Biophys J 95, 4324-4336.
34. Wu, G. Q., Wu, H. B., Fan, X. B., Zhao, R., Li, X. F., Wang, S. L., Ma, Y. H., Shen, Z. L. & Xi, T. (2010). Selective toxicity of antimicrobial peptide S-thanatin on bacteria. Peptides 31, 1669-1673.
35. Zachowski, A. (1993). Phospholipids in animal eukaryotic membranes: transverse asymmetry and movement. Biochem J 294, 1-14.