FREQ-Seq: A Rapid, Cost-Effective, Sequencing-Based Method to Determine Allele Frequencies Directly from Mixed Populations

PLoS ONE

Volumn 7, Issue 10, 2012, Pages

  Chubiz, Lon M ^a   Lee, Ming Chun ^a,c   Delaney, Nigel F ^a   Marx, Christopher J ^a,b  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

^c UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

METHANOL; OLIGONUCLEOTIDE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; FREQ SEQ; GENE AMPLIFICATION; GENE DELETION; GENE FREQUENCY; GENE INSERTION; GENE LIBRARY; GENE LOCUS; GENETIC ENGINEERING; METHYLOBACTERIUM EXTORQUENS; MICROBIAL POPULATION DYNAMICS; NONHUMAN; POLYMERASE CHAIN REACTION; POPULATION ABUNDANCE; QUANTITATIVE ANALYSIS; SEQUENCE DATABASE; SINGLE NUCLEOTIDE POLYMORPHISM; TRANSPOSON;

ALLELES; CALIBRATION; DNA TRANSPOSABLE ELEMENTS; EVOLUTION, MOLECULAR; GENE FREQUENCY; GENE LIBRARY; GENES, BACTERIAL; GENOTYPE; GENOTYPING TECHNIQUES; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; INDEL MUTATION; METHYLOBACTERIUM EXTORQUENS; MODELS, GENETIC; PLASMIDS; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; SEQUENCE ANALYSIS, DNA;

METHYLOBACTERIUM;

EID: 84868271227     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0047959     Document Type: Article

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