High-throughput bioanalytical method for analysis of synthetic cannabinoid metabolites in urine using salting-out sample preparation and LC-MS/MS

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 909, Issue , 2012, Pages 42-50

  Yanes, Enrique G ^a   Lovett, Dennis P ^a  

^a Lackland Air Force Base ^*  (United States)

Author keywords

Enzymatic hydrolysis; Salting out assisted liquid liquid extraction; Spice; Synthetic cannabinoids; UPLC; Urine

Indexed keywords

ANALYTES; ANALYTICAL METHOD; BIOANALYTICAL METHODS; CANNABINOIDS; FORENSIC LABORATORY; GLUCURONIDES; HIGH-THROUGHPUT; ISOCRATIC CONDITIONS; LC-MS/MS; LIQUID-LIQUID EXTRACTION; PLANT MATTER; PROCESSING TIME; ROOM TEMPERATURE; RUNTIMES; SALTING-OUT; SAMPLE PREPARATION; SAMPLE PREPARATION PROCEDURE; SOLID-PHASE; SYNTHETIC CANNABINOID; UPLC; URINARY METABOLITES; URINE; VOLATILE SOLVENTS;

BIOMOLECULES; BODY FLUIDS; ENZYMATIC HYDROLYSIS; GRADIENT METHODS; SPICE;

METABOLITES;

3 (1 NAPHTHOYL) 1 PENTYLINDOLE; CANNABINOID; DRUG METABOLITE; GLUCURONIDE; JWH 073; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION (PARAMETERS); HIGH THROUGHPUT SCREENING; HYDROLYSIS; LIQUID LIQUID EXTRACTION; PRIORITY JOURNAL; REACTION TIME; ROOM TEMPERATURE; SALTING OUT ASSISTED LIQUID LIQUID EXTRACTION; TANDEM MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; URINALYSIS; URINE LEVEL; VALIDATION STUDY;

CANNABINOIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; HYDROLYSIS; INDOLES; LIQUID-LIQUID EXTRACTION; NAPHTHALENES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; STREET DRUGS; TANDEM MASS SPECTROMETRY;

EID: 84868254407     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2012.10.013     Document Type: Article

References (22)

1. Mustata C., Torrens M., Pardo R., Perez C. Adicciones 2009, 21:181.
2. European Monitoring Centre for Drugs and Drug Addiction (EMCDDA), Thematic Paper - Understanding the 'Spice' Phenomenon, Luxembourg, 2009, p. 3.
3. Atwood B.K., Huffman J.W., Straiker A., Mackie K. Br. J. Pharmacol. 2010, 1.
4. Sacco L.N., Finklea K.M. Synthetic Drugs: Overview and Issues for Congress 2011, Congressional Research Service (CRS) Report for Congress.
5. Sobolevsky T., Prasolov I., Rodchenkov G. Forensic Sci. Int. 2010, 200:141.
6. Grigoryev A., Savchuk S., Melnik A., Moskaleva N., Dzhurko J., Ershov M., Nosyrev A., Vedenin A., Izotov B., Zabirova I., Rozhanets V. J. Chromatogr. B 2011, 879:1126.
7. Möller I., Wintermeyer A., Bender K., Jübner M., Thomas A., Krug O., Schänzer W., Thevis M. Drug Test. Anal. 2011, 3:609.
8. Moran C.L., Huyen Le V-H., Chimalakonda K.C., Smedley A.L., Lackey F.D., Owen S.N., Kennedy P.D., Endres G.W., Ciske F.L., Kramer J.B., Kornilov A.M., Bratton L.D., Dobrowolski P.J., Wessinger W.D., Fantegrossi W.E., Prather P.L., James L.P., Radominska-Pandya A., Moran J.H. Anal. Chem. 2011, 83:4228.
9. ElSohly M.A., Gul W.G., ElSohly K.M., Murphy T.P., Madgula V.L.M., Khan S.I. J. Anal. Toxicol. 2011, 35:487.
10. Chimalakonda K.C., Moran C.L., Kennedy P.D., Endres G.W., Uzieblo A., Dobrowolski P.J., Fifer E.K., Lapoint J., Nelson L.S., Hoffman R.S., James L.P., Radominska-Pandya A., Moran J.H., Solid-Phase Anal. Chem. 2011, 83:6381.
11. A. Rigdon, P. Kennedy, T. Kahler, LC/MS/MS analysis of metabolites of synthetic cannabinoids JWH-018 and JWH-073 in urine, Restek Corp. Technical Bulletin #CFAN1383.
12. Matuszewski B.K., Constanzer M.L., Chavez-Eng C.M. Anal. Chem. 2003, 75:3019.
13. J. Hughes, P. Moorman, Confirmation by triple quadrupole LC/MS/MS for HHS-compliant workplace urine drug testing, Website: (accessed 24.09.12). http://www.biochemicaldiagnostics.com/pdffiles/hhscompliantworkpacedrugtesting.pdf.
14. Van Eeckhaut A., Lanckmans K., Sarre S., Smolders I., Michotte Y. J. Chromatogr. B 2009, 877:2198.
15. Zhang J., Wu H., Kim E., El-Shourbagy T.A. Biomed. Chromatogr. 2009, 23:419.
16. Zhang J., Rodila R., Gage E., Hautman M., Fan L., King L.L., Wu H., El-Shourbagy T.A. Anal. Chim. Acta 2010, 661:167.
17. R.E. Majors, Salting-out liquid-liquid extraction (SALLE), Website: (accessed 24.09.12). http://www.chromatographyonline.com/lcgc/article/articleDetail.jsp%3Fid=613590.
18. Wu H., Zhang J., Norem K., El-Shourbagy T.A. J. Pharm. Biomed. Anal. 2008, 48:1243.
19. Zhao F.-J., Tang H., Zhang Q.-H., Yang J., Davey A.K., Wang J. J. Chromatogr. B 2012, 881-882:119.
20. Rustum A.M. J. Chromatogr. B Biomed. Sci. Appl. 1989, 489:345.
21. Rustum A.M. J. Chromatogr. B Biomed. Sci. Appl. 1989, 489:365.
22. B.K. Logan, S.L. Kacinko, M.M. McMullin, A. Xu, R.A. Middleberg, Technical bulletin: identification of primary JWH-018 and JWH-073 metabolites in human urine, 2011.