Liquid chromatography-tandem mass spectrometry analysis of urine specimens for K2 (JWH-018) metabolites

Journal of Analytical Toxicology

Volumn 35, Issue 7, 2011, Pages 487-495

  ElSohly, Mahmoud A ^a,b,c   Gul, Waseem ^a,b   ElSohly, Kareem M ^a   Murphy, Timothy P ^a   Madgula, Vamsi L M ^b   Khan, Shabana I ^b,c  

^a ElSohly Laboratories Incorporated   (United States)

^b UNIVERSITY OF MISSISSIPPI   (United States)

^c UNIVERSITY OF MISSISSIPPI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3 (1 NAPHTHOYL) 1 PENTYLINDOLE; 6 HYDROXY PENTANYL; CANNABIS; DRUG METABOLITE; JWH 073; N 5 HYDROXY PENTANYL; N PENTANOIC ACID; UNCLASSIFIED DRUG;

ARTICLE; CANNABIS ADDICTION; FORENSIC TOXICOLOGY; HUMAN; HUMAN CELL; IN VITRO STUDY; LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY; LIVER MICROSOME; MASS SPECTROMETRY; URINALYSIS;

CANNABIS SATIVA;

EID: 80052616750     PISSN: 01464760     EISSN: 19452403     Source Type: Journal    
DOI: 10.1093/anatox/35.7.487     Document Type: Article

References (14)

1. 9-THC-9-COOH. J. Anal. Toxicol. 21: 240-242 (1997).
2. S.C. Tsai, M.A. ElSohly, T. Dubrovsky, B. Twarowska, J. Towt, and S.J. Salamone. Determination of five abused drugs in nitrateadulterated urine by immunoassays and gas chromatography-mass spectrometry. J. Anal. Toxicol. 22(6): 474-480 (1998).
3. Mandatory Guidelines for Federal Workplace Drug Testing Programs published in the Federal Register on April 13, 2004 (69 FR 19644), effective November 1, 2004.
4. L.P. Raymon and H.C. Walls. Pharmacology of cannabinoids. In Marijuana and the Cannabinoids. Humana Press, New York, NY, 2007, pp 97-123.
5. 8-tetrahydrocannabinols: new selective ligands for the CB2 receptor. Bioorg. Med. Chem. 10: 4119-4129 (2002).
6. R. Lindigkeit, A. Boehme, I. Eiserloh, M. Luebbecke, M. Wiggermann, L. Ernst, and T. Beuerle. Spice: a never ending story? Forensic Sci. Int. 191: 58-63 (2009).
7. N. Uchiyama, R. Kikura-Hanajiri, J. Ogata, and Y. Goda. Chemical analysis of synthetic cannabinoids as designer drugs in herbal products. Forensic Sci. Int. 198: 31-38 (2010).
8. J. Teske, J.P. Weller, A. Fieguth, T. Rothämel, Y. Schulz, and H.D. Tröger. Sensitive and rapid quantification of the cannabinoid receptor agonist naphthalene-1-yl-(1-pentylindol-3-yl) methanone (JWH-018) in human serum by liquid chromatography-tandem mass spectrometry. J. Chromatogr. B 878(27): 2659-2663 (2010).
9. T. Sobolevsky, I. Prasolov, and G. Rodchenkov. Detection of JWH-018 metabolites in smoking mixture post-administration urine. Forensic Sci. Int. 200: 141-147 (2010).
10. A. Wintermeyer, I. Möller, M. Thevis, M. Jübner, J. Beike, M.A. Rothschild, and K. Bender. In vitro phase I metabolism of the synthetic cannabimimetic JWH-018. Anal. Bioanal. Chem. 398: 2141-2153 (2010).
11. Q. Zhang, P. Ma, R.B. Cole, and G. Wang. Identification of in vitro metabolites of JWH-015, an aminoalkylindole agonist for the peripheral cannabinoid receptor (CB2) by HPLC-MS/MS. Anal. Bioanal. Chem. 386: 1345-1355 (2006).
12. I. Möller, A. Wintermeyer, K. Bender, M. Jübner, A. Thomas, O. Krug, W. Schänzer, and M. Thevis. Screening for the synthetic cannabinoid JWH-018 and its major metabolites in human doping controls. Drug Test. Anal., in press. doi;10.1002/dta.158.
13. P.J. Kilford, R. Stringer, B. Sohal, J.B. Houston, and A. Galetin. Prediction of drug clearance by glucuronidation from in vitro data: use of combined cytochrome P450 and UDP-glucuronosyltransferase cofactors in alamethicin-activated human liver microsomes. Drug Metab. Dispos. 37(1): 82-89 (2009).
14. V.L. Madgula, B. Avula, R.S. Pawar, Y.J. Shukla, I.A. Khan, L.A. Walker, and S.I. Khan. Characterization of in vitro pharmacokinetic properties of hoodigogenin A from Hoodia gordonii. Planta Medica 76(1): 62-69 (2010).