Biochemical characterization of a new recombinant TNF receptor-hyFc fusion protein expressed in CHO cells

Protein Expression and Purification

Volumn 87, Issue 1, 2013, Pages 17-26

  Lee, Jung Hwan ^a,d   Yeo, Jiwoo ^a   Park, Hyoung Seo ^a   Sung, Gong ^a   Lee, Sung Hee ^a   Yang, Se Hwan ^b   Sung, Young Chul ^b,c   Kang, Ju Hee ^d   Park, Chang Shin ^d  

^a Dong A Pharmaceutical Company   (South Korea)

^b POSTECH Biotech Center   (South Korea)

^c Pohang University of Science and Technology (POSTECH)   (South Korea)

^d Inha University School of Medicine   (South Korea)

Author keywords

Fc fusion protein; Hybrid Fc; N Glycan; TNF blocker; TNF ; TNFR hyFc

Indexed keywords

HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G; MONOSACCHARIDE; PEPTIDE FRAGMENT; TNFR FC FUSION PROTEIN; TNFR-FC FUSION PROTEIN; TUMOR NECROSIS FACTOR ALPHA; TUMOR NECROSIS FACTOR RECEPTOR;

ANIMAL; ARTICLE; BIOSYNTHESIS; CARBOHYDRATE ANALYSIS; CELL DEATH; CHEMISTRY; CHO CELL; CONFORMATION; DRUG ANTAGONISM; DRUG EFFECT; GENETICS; GLYCOSYLATION; HAMSTER; MOLECULAR GENETICS; MOLECULAR WEIGHT; MOUSE; PEPTIDE MAPPING; PHYSIOLOGY; PROTEIN PROCESSING; PROTEIN STABILITY; SEQUENCE ANALYSIS;

ANIMALS; CARBOHYDRATE CONFORMATION; CARBOHYDRATE SEQUENCE; CELL DEATH; CHO CELLS; CRICETINAE; GLYCOSYLATION; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; MICE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MONOSACCHARIDES; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STABILITY; RECEPTORS, TUMOR NECROSIS FACTOR; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, PROTEIN; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84868243646     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.09.001     Document Type: Article

References (36)

1. D. Tracey, L. Klareskog, E.H. Sasso, J.G. Salfeld, and P.P. Tak Tumor necrosis factor antagonist mechanisms of action: a comprehensive review Pharmacol. Ther. 117 2008 244 279
2. B.J. Scallon, H. Trinh, M. Nedelman, F.M. Brennan, M. Feldmann, and J. Ghrayeb Functional comparisons of different tumor necrosis factor receptor/IgG fusion protein Cytokine 7 1995 759 770
3. G. Cunnane, M. Doran, and B. Bresnihan Infections and biological therapy in rheumatoid arthritis Best Pract. Res. Clin. Rheumatol. 17 2003 345 363
4. I. Nestorov Clinical pharmacokinetics of TNF antagonist: how do they differ? Semin. Arthritis Rheum. 34 2004 12 18
5. B. Goffe, and J.C. Cather Etanercept: an overview J. Am. Acad. Dermatol. 49 2003 s105 s111
6. K. Schriebl, E. Trummer, C. Lattenmayer, R. Weik, R. Kunert, D. Muller, H. Katinger, and K. Vorauer-Uhl Biochemical characterization of rhEpo-Fc fusion protein expressed in CHO cells Protein Expr. Purif. 49 2006 265 275 (Pubitemid 44416228)
7. M. Zhang, D. Yu, C. Yang, Q. Xia, W. Li, and B. Liu The pharmacology study of a new recombinant human VEGF-Fc fusion protein on experimental choroidal neonascularization Pharm. Res. 26 2009 204 210
8. N. Soltani, M. Kimar, Y. Glinka, G.J. Prud'homme, and Q. Wang In vivo expression of GLP-1/IgG-Fc fusion protein enhances beta-cell mass and protects against streptozotocin-induced diabetes Gene Ther. 14 2007 981 988 (Pubitemid 46865509)
9. S.M. Chamow, and A. Ashkenazi Immunoadhesions: principles and applications Trends Biotechnol. 14 1996 52 60
10. X. Mariette Emerging biological therapies in rheumatoid arthritis Joint Bone Spine 71 2004 470 474 (Pubitemid 39626712)
11. J.M. Weinberg An overview of infliximab, etanercept, efalizumab, and alefacept as biologic therapy for psoriasis Clin. Ther. 25 2003 2487 2505 (Pubitemid 37373927)
12. E.E. Idusogie, P.Y. Wong, L.G. Presta, H. Gazzano-Santoro, and K. Totpal Engineered antibodies with increased activity to recruit complement J. Immunol. 166 2001 2571 2575 (Pubitemid 32173494)
13. H. Schroeder, and L. Cavacini Structure and function of immunoglobulins J. Allergy Clin. Immunol. 125 2010 41 52
14. X.Y. Liu, L.M. Pop, and E.S. Vitetta Engineering therapeutic monoclonal antibodies Immunol. Rev. 222 2008 9 27 (Pubitemid 351430363)
15. L.G. Presta Selection, design, and engineering of therapeutic antibodies J. Allergy Clin. Immunol. 116 2005 731 736 (Pubitemid 41400532)
16. J. Schuurman, G.J. Perdk, A.D. Gorter, and R.C. Aalberse The inter-heavy chain disulfide bonds of IgG4 are in equilibrium with intra-chain disulfide binds Mol. Immunol. 38 2001 1 8 (Pubitemid 32718637)
17. Z. An, G. Forrest, R. Moore, M. Cukan, P. Haytko, L. Huang, S. Vitelli, J.Z. Zhao, P. Lu, J. Hua, C.R. Gibson, B.R. Harvey, D. Montgomery, D. Zaller, F. Wang, and W. Strohl IgG2m4, an engineered antibody isotope with reduced Fc fuction mAbs 1 2009 572 579
18. R.P. Rother, S.A. Mojcik, R.A. Brodsky, and L. Bell Discovery and development of the complement inhibitor eculizumab for the treatment of paradoxymal nocturnal hemoglobinuria Nat. Biotechnol. 25 2007 1256 1264 (Pubitemid 350076509)
19. S.J. Im, S.I. Yang, S.H. Yang, D.H. Choi, S.Y. Choi, H.S. Kim, D.S. Jang, K.S. Jin, Y.K. Chung, S.H. Kim, S.H. Paik, Y.C. Park, M.K. Chung, Y.B. Kim, K.H. Han, K.Y. Choi, and Y.C. Sung Natural form of noncytolytic flexible human Fc as a long-acting carrier of agonistic ligand, erythropoietin PLoS One 6 2011 E24574
20. D.J. Capon, N. Kaneko, T. Yoshimori, T. Shimada, F.M. Wurm, P.K. Hwang, X. Tong, S.A. Adams, G. Simmons, T.A. Sato, and K. Tanaka Flexible antibodies with nonprotein hinge Proc. Jpn. Acad. Ser. B: Phys. Biol. Sci. 87 2011 603 616
21. K.D. Speicher, N. Gorman, and D.W. Speicher N-terminal sequence analysis of proteins and peptide Curr. Protoc. Protein Sci. 57 2009 11.10.1 11.10.31
22. S.A. Cohen, and D.P. Michaud Synthesis of a fluorescent derivatizing reagent, 6- aminoquinolyl-N-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high performance liquid chromatography Anal. Biochem. 211 1993 279 287 (Pubitemid 23207730)
23. I. Molnar-Perl Tryptophan analysis in peptides and proteins, mainly by liquid chromatography J. Chromatogr. A 763 1997 1 10 (Pubitemid 27149968)
24. C.C. Ip, V. Manam, R. Hepler, and J.P. Hennessey Carbohydrate composition analysis of bacterial polysaccharides: optimized acid hydrolysis condition for HPAEC-PAD analysis Anal. Biochem. 201 1992 343 349
25. S. Birken, Y. Maydelman, M.A. Gawinowicz, A. Pound, Y. Liu, and A.S. Hartree Isolation and characterization of human pituitary chorionic gonadotropin Endocrionology 137 1996 1402 1411 (Pubitemid 26095259)
26. D.I. Papac, J.B. Briggs, E.T. Chin, and A.J. Jones A high-throughput microscale method to release N-linked oligosaccharides from glycoproteins for matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis Glycobiology 8 1998 445 454 (Pubitemid 28282624)
27. A. Gervais, Y.A. Hammel, S. Pelloux, P. Lepage, G. Baer, N. Carte, O. Sorokine, J.M. Strub, R. Koerner, E. Leize, and A.V. Dorsselaer Glycosylation of human recombinant gonadotrophins: characterization and batch-to-batch consistency Glycobiology 13 2003 179 189 (Pubitemid 36511753)
28. M. Wuhrer, A.M. Deelder, and C.H. Hokke Protein glycosylation analysis by liquid chromatography-mass spectrometry J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 825 2005 124 133 (Pubitemid 41423595)
29. J. Van Beeumen, G. Van Driessche, F. Huitema, J.A. Duine, and G.W. Canters N-terminal heterogeneity of methylamine dehydrogenase from Thiobacillus versutus FEBS Lett. 333 1993 188 192 (Pubitemid 23306966)
30. M. Fountoulakis, and H. Lahm Hydrolysis and amino acid composition analysis of proteins J. Chromatogr. A. 826 1998 109 134 (Pubitemid 28543135)
31. P.C. Taylor Pharmacology of TNF blockade in rheumatoid arthritis and other chronic inflammatory diseases Curr. Opin. Pharmacol. 10 2010 308 315
32. Z. Kaymakcalan, P. Sakorafas, S. Bose, S. Scesney, L. Xiong, D.K. Hanzatian, J. Salfeld, and E.H. Sasso Comparisons of affinities, avidities, and complement activation of adalimumab, infliximab, and etanercept in binding to soluble and membrane tumor necrosis factor Clin. Immunol. 131 2009 308 316
33. V. Ghetie, and E.S. Ward FcRn: the MHC class I-related receptor that is more than an IgG transporter Immunol. Today 18 1997 592 598 (Pubitemid 28012280)
34. K.H. Roux, L. Strelets, O.H. Brekke, and T.E. Michaelesn Comparision of the ability of human IgG3 hinge mutants, IgM, IgE, and IgA2, to form small immune complexes: a role for flexibility and geometry J. Immunol. 161 1998 4083 4090 (Pubitemid 28468987)
35. G.A. Loset, K.H. Roux, P. Zhu, T.E. Michaelsen, and I. Standlie Differential segmental flexibility and reach dictate the antigen binding mode of chimeric igD and IgM: implications for the function of the B cell receptor J. Immunol. 172 2004 2925 2934 (Pubitemid 38263679)
36. K. Chen, and A. Cerutti The function and regulation of immunoglobulin D Curr. Opin. Immunol. 23 2011 345 352