Autophagosomes accumulate in differentiated and hypertrophic adipocytes in a p53-independent manner

Biochemical and Biophysical Research Communications

Volumn 427, Issue 4, 2012, Pages 758-763

  Mikami, Kentaro ^a   Okita, Naoyuki ^a   Tokunaga, Yuki ^a   Ichikawa, Tomoyo ^a   Okazaki, Tatsuya ^a   Takemoto, Kanako ^a   Nagai, Wataru ^a   Matsushima, Shingo ^a   Higami, Yoshikazu ^a  

^a TOKYO UNIVERSITY OF SCIENCE   (Japan)

Author keywords

Adipocyte; Autophagic flux; Autophagosome; Differentiation; Obesity; P53

Indexed keywords

ETOPOSIDE; PROTEIN P53; RAPAMYCIN; TRIACYLGLYCEROL;

ADIPOCYTE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; AUTOPHAGOSOME; AUTOPHAGY; CONTROLLED STUDY; IN VITRO STUDY; LIPID DIET; MALE; MOUSE; NONHUMAN; OBESITY; PRIORITY JOURNAL; PROTEIN EXPRESSION; WHITE ADIPOSE TISSUE;

3T3-L1 CELLS; ADIPOCYTES; ANIMALS; AUTOPHAGY; CELL DIFFERENTIATION; CELL ENLARGEMENT; FASTING; MICE; MICE, KNOCKOUT; TUMOR SUPPRESSOR PROTEIN P53;

MUS;

EID: 84868202422     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.09.134     Document Type: Article

References (27)

1. Levine B., Kroemer G. Autophagy in the pathogenesis of disease. Cell 2008, 132:27-42.
2. Tanida I., Minematsu-Ikeguchi N., Ueno T., Kominami E. Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 2005, 1:84-91.
3. Mizushima N., Klionsky D.J. Protein turnover via autophagy: implications for metabolism. Annu. Rev. Nutr. 2007, 27:19-40.
4. Mizushima N., Yoshimori T., Levine B. Methods in mammalian autophagy research. Cell 2010, 140:313-326.
5. Vogelstein B., Lane D., Levine A.J. Surfing the p53 network. Nature 2000, 408:307-310.
6. Matoba S., Kang J.G., Patino W.D., Wragg A., Boehm M., Gavrilova O., Hurley P.J., Bunz F., Hwang P.M. p53 regulates mitochondrial respiration. Science 2006, 312:1650-1653.
7. Green D.R., Chipuk J.E. P53 and metabolism: inside the TIGAR. Cell 2006, 126:30-32.
8. Feng Z., Zhang H., Levine A.J., Jin S. The coordinate regulation of the p53 and mTOR pathways in cells. Proc. Natl. Acad. Sci. USA 2005, 7:8204-8209.
9. Maiuri M.C., Malik S.A., Morselli E., Kepp O., Criollo A., Mouchel P., Carnuccio R., Kroemer G. Stimulation of autophagy by the p53 target gene Sestrin2. Cell Cycle 2009, 8:1571-1576.
10. Crighton D., Wilkinson S., O'Prey J., Syed N., Smith P., Harrison P.R., Gasco M., Garrone O., Crook T., Ryan K.M. DRAM, a p53-induced modulator of autophagy, is critical for apoptosis. Cell 2006, 126:121-134.
11. Novikoff A.B., Novikoff P.M., Rosen O.M., Rubin C.S. Organelle relationships in cultured 3T3-L1 preadipocytes. J. Cell Biol. 1980, 87:180-196.
12. Baerga R., Zhang Y., Chen P., Goldman S., Jin S. Targeted deletion of autophagy-related 5 (atg5) impairs adipogenesis in a cellular model and in mice. Autophagy 2009, 5:1118-1130.
13. Singh R., Xiang Y., Wang Y., Baikati K., Cuervo A.M., Luu Y.K., Tang Y., Pessin J.E., Schwartz G.J., Czaja M.J. Autophagy regulates adipose mass and differentiation in mice. J. Clin. Invest. 2009, 119:3329-3339.
14. Zhanga Y., Goldmana S., Baergaa R., Zhaoa Y., Komatsub M., Jina S. Adipose-specific deletion of autophagy-related gene 7 (atg7) in mice reveals a role in adipogenesis. Proc. Natl. Acad. Sci. USA 2009, 106:19860-19865.
15. Goldman S., Zhang Y., Jin S. Autophagy and adipogenesis Implications in obesity and type II diabetes. Autophagy 2010, 6:179-181.
16. Singh R., Kaushik S., Wang Y., Xiang Y., Novak I., Komatsu M., Tanaka K., Cuervo A.M., Czaja M.J. Autophagy regulates lipid metabolism. Nature 2009, 458:1131-1135.
17. Matsushima S., Okita N., Oku M., Nagai W., Kobayashi M., Higami Y. An Mdm2 antagonist, Nutlin-3a, induces p53-dependent and proteasome-mediated poly(ADP-ribose) polymerase1 degradation in mouse fibroblasts. Biochem. Biophys. Res. Commun. 2011, 407:557-561.
18. Kamura T., Hara T., Matsumoto M., Ishida N., Okumura F., Hatakeyama S., Yoshida M., Nakayama K., Nakayama K.I. Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27Kip1 at G1 phase. Nat. Cell Biol. 2004, 6:1229-1235.
19. Dirac A.M., Bernards R. Reversal of senescence in mouse fibroblasts through lentiviral suppression of p53. J. Biol. Chem. 2003, 278:11731-11734.
20. Morita S., Kojima T., Kitamura T. Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther. 2000, 7:1063-1066.
21. Nishino N., Tamori Y., Tateya S., Kawaguchi T., Shibakusa T., Mizunoya W., Inoue K., Kitazawa R., Kitazawa S., Matsuki Y., Hiramatsu R., Masubuchi S., Omachi A., Kimura K., Saito M., Amo T., Ohta S., Yamaguchi T., Osumi T., Cheng J., Fujimoto T., Nakao H., Nakao K., Aiba A., Okamura H., Fushiki T., Kasuga M. FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets. J. Clin. Invest. 2008, 118:2808-2821.
22. Jung C.H., Ro S., Cao J., Otto N.M., Kim D. mTOR regulation of autophagy. FEBS Lett. 2010, 584:1287-1295.
23. Scherz-Shouval R., Weidberg H., Gonen C., Wilder S., Elazar Z., Oren M. p53-Dependent regulation of autophagy protein LC3 supports cancer cell survival under prolonged starvation. Proc. Natl. Acad. Sci. USA 2010, 107:18511-18516.
24. Yahagi N., Shimano H., Matsuzaka T., Najima Y., Sekiya M., Nakagawa Y., Ide T., Tomita S., Okazaki H., Tamura Y., Iizuka Y., Ohashi K., Gotoda T., Nagai R., Kimura S., Ishibashi S., Osuga J., Yamada N. p53 activation in adipocytes of obese mice. J. Biol. Chem. 2003, 278:25395-25400.
25. Furukawa S., Fujita T., Shimabukuro M., Iwaki M., Yamada Y., Nakajima Y., Nakayama O., Makisima M., Matsuda M., Sgimomura I. Increased oxidative stress in obesity and its impact on metabolic syndrome. J. Clin. Invest. 2004, 114:1752-1761.
26. Lieberman A.P., Puertollano R., Raben N., Slaugenhaupt S., Walkley S.U., Ballabio A. Autophagy in lysosomal storage disorders. Autophagy 2012, 8:719-730.
27. Yoshizaki T., Kusunoki C., Kondo M., Yasuda M., Kume S., Morino K., Sekine O., Ugi S., Uzu T., Nishio Y., Kashiwagi A., Maegawa H. Autophagy regulates inflammation in adipocytes. Biochem. Biophys. Res. Commun. 2012, 417:352-357.